REVIEW

Biological Function and Application of Schizochytrium in Livestock Production

  • GONG Jiatai ,
  • HU Ruizhi ,
  • YANG Xizi ,
  • WU Shusong
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  • Hunan Co-Innovation Center of Safety Animal Production, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China

Received date: 2021-08-15

  Online published: 2022-03-14

Abstract

Schizochytrium is a single-celled marine fungus that can produce protein, fat and other nutrients under different fermentation techniques, and thus can be used as a feed source. In addition, Schizochytrium can produce active substances such as docosahexaenoic acid (DHA), squalene, and astaxanthin, especially high level of DHA. Supplementation of Schizochytrium powder to animal feed can improve immunity and the ability against oxidative stress and inflammation. In this paper, the main nutritional components, fermentation technology, biological functions of main active substances of Schizochytrium and its application in livestock production were reviewed.

Cite this article

GONG Jiatai , HU Ruizhi , YANG Xizi , WU Shusong . Biological Function and Application of Schizochytrium in Livestock Production[J]. Chinese Journal of Animal Nutrition, 2022 , 34(3) : 1476 -1485 . DOI: 10.3969/j.issn.1006-267x.2022.03.012

References

[1] YAGUCHI T,TANAKA S,YOKOCHI T,et al.Production of high yields of docosahexaenoic acid by Schizochytrium sp. strain SR21[J].Journal of the American Oil Chemists' Society,1997,74(11):1431-1434.  
[2] RICHARD D,KEFI K,BARBE U,et al.Polyunsaturated fatty acids as antioxidants[J].Pharmacological Research,2008,57(6):451-455.  
[3] OHTSUKA Y,OKADA K,YAMAKAWA Y,et al.ω-3 fatty acids attenuate mucosal inflammation in premature rat pups[J].Journal of Pediatric Surgery,2011,46(3):489-495.  
[4] 朱路英,张学成,王淑芳,等.一种海洋真菌——裂殖壶菌的营养成分分析[J].食品科学,2009,30(24):272-275. ZHU L Y,ZHANG X C,WANG S F,et al.Analysis of nutritional components of a marine fungus:Schizochytrium limanium[J].Food Science,2009,30(24):272-275.(in Chinese)
[5] 史超.裂殖壶菌DHA油脂的发酵生产及油脂组成分析[D].硕士学位论文.大连:大连工业大学,2018. SHI C.Fermentation production and component analysis of DHA-rich lipid by Schizochytrium sp[D].Master's Thesis.Dalian:Dalian University of Technology,2018.(in Chinese)
[6] JIANG Y,FAN K W,WONG R T Y,et al.Fatty acid composition and squalene content of the marine microalga Schizochytrium mangrovei[J].Journal of Agricultural and Food Chemistry,2004,52(5):1196-1200.  
[7] AKI T,HACHIDA K,YOSHINAGA M,et al.Thraustochytrid as a potential source of carotenoids[J].Journal of the American Oil Chemists' Society,2003,80(8):789.
[8] 宋泽,彭雍博,宋悦凡,等.裂殖壶菌营养成分及其多糖特征分析[J].大连海洋大学学报,2019,34(2):247-251. SONG Z,PENG Y B,SONG Y F,et al.Analysis of nutrient composition and polysaccharide characteristics of fungus Schizochytrium sp[J].Journal of Dalian Fisheries University,2019,34(2):247-251.(in Chinese)
[9] 曹欢,王培培,吴建东,等.裂壶藻(Schizochytrium limacinum)多糖的提取分离及其结构特性[J].中国海洋药物,2011,30(6):1-5. CAO H,WANG P P,WU J D,et al.Extraction,isolation and structural characterization of polysaccharides from Schizochytrium limacinum[J].Chinese Journal of Marine Drugs,2011,30(6):1-5.(in Chinese)
[10] ŠAJBIDOR J.Effect of some environmental factors on the content and composition of microbial membrane lipids[J].Critical Reviews in Biotechnology,1997,17(2):87-103.  
[11] CHIHIB N E,TIERNY Y,MARY P,et al.Adaptational changes in cellular fatty acid branching and unsaturation of Aeromonas species as a response to growth temperature and salinity[J].International Journal of Food Microbiology,2005,102(1):113-119.  
[12] SONG X J,ZHANG X C,KUANG C H,et al.Optimization of fermentation parameters for the biomass and DHA production of Schizochytrium limacinum OUC88 using response surface methodology[J].Process Biochemistry,2007,42(10):1391-1397.  
[13] 龚定芳.高产DHA裂殖壶菌的诱变选育及转录组分析[D].硕士学位论文.无锡:江南大学,2019. GONG D F.Mutation breeding and transcriptome analysis of Schizochytrium with high-yield DHA production[D].Master's Thesis.Wuxi:Jiangnan University,2019.(in Chinese)
[14] 付杰.高产DHA裂壶藻突变株的选育及生物学特性的研究[D].硕士学位论文.武汉:武汉轻工大学,2017. FU J.Research on the breeding and biological characteristics of Schizochytrium sp.for DHA production[D].Master's Thesis.Wuhan:Wuhan Polytechnic University,2017.(in Chinese)
[15] QU L,REN L J,SUN G N,et al.Batch,fed-batch and repeated fed-batch fermentation processes of the marine thraustochytrid Schizochytrium sp. for producing docosahexaenoic acid[J].Bioprocess and Biosystems Engineering,2013,36(12):1905-1912.  
[16] 王申强,罗玮,姜易彤,等.外源添加剂促进裂殖壶菌合成DHA[J].生物加工过程,2013,11(5):21-25. WANG S Q,LUO W,JIANG Y T,et al.Enhancing DHA synthesis in Schizochytrium limacinum by exogenous additives[J].Chinese Journal of Bioprocess Engineering,2013,11(5):21-25.(in Chinese)
[17] 张良,罗杰,樊涛,等.不同消泡剂对裂殖壶菌生长的影响[J].发酵科技通讯,2020,49(3):175-180. ZHANG L,LUO J,FAN T,et al.Effects of different defoaming agents on the growth of Schizochytrium[J].Bulletin of Fermentation Science and Technology,2020,49(3):175-180.(in Chinese)
[18] SONG X J,ZANG X N,ZHANG X C.Production of high docosahexaenoic acid by Schizochytrium sp. using low-cost raw materials from food industry[J].Journal of Oleo Science,2015,64(2):197-204.  
[19] YU X J,YU Z Q,LIU Y L,et al.Utilization of high-fructose corn syrup for biomass production containing high levels of docosahexaenoic acid by a newly isolated Aurantiochytrium sp. YLH70[J].Applied Biochemistry and Biotechnology,2015,177(6):1229-1240.  
[20] LIANG Y N,SARKANY N,CUI Y,et al.Use of sweet sorghum juice for lipid production by Schizochytrium limacinum SR21[J].Bioresource Technology,2010,101(10):3623-3627.  
[21] SHAFIQ M,ZEB L,CUI G N,et al.High-density ph-auxostat fed-batch culture of Schizochytrium limacinum sr21 with acetic acid as a carbon source[J].Applied Biochemistry and Biotechnology,2020,192(4):1163-1175.  
[22] BHATIA S K,YANG Y H.Microbial production of volatile fatty acids:current status and future perspectives[J]. Reviews in Environmental Science and Bio/Technology,2017,16(2):327-345.  
[23] EL-GAMMAL M,ABOU-SHANAB R,ANGELIDAKI I,et al.High efficient ethanol and VFA production from gas fermentation:effect of acetate,gas and inoculum microbial composition[J].Biomass and Bioenergy,2017,105:32-40.
[24] AHMAD I,HELLEBUST J A,et al.Regulation of chloroplast development by nitrogen source and growth conditions in a Chlorella protothecoides strain[J].Plant physiology,1990,94(3):944-949.  
[25] DROOP M R.Heterotrophy of carbon[M]//STEWART W D P.Algal physiology and biochemistry.London:Blackwell Scientific Publications,1974:530-559.
[26] NEILSON A H,LEWIN R A,et al.The uptake and utilization of organic carbon by algae:an essay in comparative biochemistry[J].Phycologia,1974,13(3):227-264.  
[27] DE SWAAF M E,SIJTSMA L,PRONK J T.High-cell-density fed-batch cultivation of the docosahexaenoic acid producing marine alga Crypthecodinium cohnii[J].Biotechnology and Bioengineering,2003,81(6):666-672.  
[28] BOYLE N R,MORGAN J A.Flux balance analysis of primary metabolism in Chlamydomonas reinhardtii[J].BMC Systems Biology,2009,3:4.
[29] GONG Z W,SHEN H W,ZHOU W T,et al.Efficient conversion of acetate into lipids by the oleaginous yeast Cryptococcus curvatus[J].Biotechnology for Biofuels,2015,8:189.
[30] LIAN J N,GARCIA-PEREZ M,COATES R,et al.Yeast fermentation of carboxylic acids obtained from pyrolytic aqueous phases for lipid production[J].Bioresource Technology,2012,118:177-186.
[31] SHAFIQ M,ZEB L,CUI G N,et al.High-density pH-auxostat fed-batch culture of Schizochytrium limacinum SR21 with acetic acid as a carbon source[J].Applied Biochemistry and Biotechnology,2020,192(4):1163-1175.  
[32] PYLE D J.Use of biodiesel-derived crude glycerol for the production of omega-3 polyunsaturated fatty acids by the microalga Schizochytrium limacinum[D].Master's Thesis.Blacksburg,VA:Virginia Polytechnic Institute and State University,2008.
[33] SHU Q,GAO J X,NAWAZ Z,et al.Synthesis of biodiesel from waste vegetable oil with large amounts of free fatty acids using a carbon-based solid acid catalyst[J].Applied Energy,2010,87(8):2589-2596.  
[34] TAUFIQ-YAP Y H,AHMAD FARABI M S,SYAZWANI O N,et al.Sustainable production of bioenergy[M].Singapore:Springer Singapore,2020:541-561.
[35] CHI Z Y,PYLE D,WEN Z Y,et al.A laboratory study of producing docosahexaenoic acid from biodiesel-waste glycerol by microalgal fermentation[J].Process Biochemistry,2007,42(11):1537-1545.  
[36] LUNG Y T,TAN C H,SHOW P L,et al.Docosahexaenoic acid production from crude glycerol by Schizochytrium limacinum SR21[J].Clean Technologies and Environmental Policy,2016,18(7):2209-2216.  
[37] 李婧.裂殖壶菌利用混合碳源发酵生产DHA的研究[D].硕士学位论文.无锡:江南大学,2015. LI J.A strategy of highly DHA production by Aurantiochytrium limacinum SR21 with mixed carbon sources[D]. Master's Thesis.Wuxi:Jiangnan University,2015.(in Chinese)
[38] REN L J,SUN L N,ZHUANG X Y,et al.Regulation of docosahexaenoic acid production by Schizochytrium sp.:effect of nitrogen addition[J].Bioprocess and Biosystems Engineering,2014,37(5):865-872.  
[39] XU J,ZHU Y J,LI H C,et al.Alanine mother liquor as a nitrogen source for docosahexaenoic acid production by Schizochytrium sp. B4D1[J].Electronic Journal of Biotechnology,2018,35:10-17.
[40] 王澍,吕小义,张静雯,等.不同碳氮源浓度和培养温度对裂殖壶菌产DHA的影响[J].中国油脂,2015,40(10):74-77. WANG S,LV X Y,ZHANG J W,et al.Impacts of different concentrations of carbon and nitrogen sources and culture temperature on DHA production by Schizochytrium sp[J].China Oils and Fats,2015,40(10):74-77.(in Chinese)
[41] 贾俊乾,张秋红,陆向红,等.pH值对裂殖壶菌发酵生产二十二碳六烯酸的影响[J].生物学杂志,2015,32(4):16-19. JIA J Q,ZHANG Q H,LIU X H,et al.Impact of pH on docosahexaenoic acid (DHA) production in fermentation of Schizochytrium limacinum[J].Journal of Biology,2015,32(4):16-19.(in Chinese)
[42] 康晶,郑志永,詹晓北,等.氮源和溶氧限制对裂殖壶菌Schizochytrium limacinum SR21合成二十二碳六烯酸的影响[J].工业微生物,2013,43(2):58-63. KANG J,ZHENG Z Y,ZHAN X B,et al.Effects of nitrogen starvation and oxygen limitation on docosahexenoic acid production by Schizochytrium limacinum SR21[J].Industrial Microbiology,2013,43(2):58-63.(in Chinese)
[43] LI Z P,LING X P,ZHOU H,et al.Screening chemical modulators of benzoic acid derivatives to improve lipid accumulation in Schizochytrium limacinum SR21 with metabolomics analysis[J].Biotechnology for Biofuels,2019,12:209.
[44] 林源锋,谢鑫磊,付杰,等.化学添加剂对裂壶藻突变株发酵产DHA的影响[J].食品工业科技,2017,38(22):125-129. LIN Y F,XIE X L,FU J,et al.Effect of chemical modulators on DHA production by fermentation of Schizochytrium limacinum mutant strain[J].Science and Technology of Food Industry,2017,38(22):125-129.(in Chinese)
[45] SAVCHENKO O,XING J D,BURRELL M,et al.Impact of low-intensity pulsed ultrasound on the growth of Schizochytrium sp. for omega-3 production[J].Biotechnology and Bioengineering,2021,118(1):319-328.  
[46] BAGGA D,WANG L,FARIAS-EISNER R,et al.Differential effects of prostaglandin derived from omega-6 and omega-3 polyunsaturated fatty acids on COX-2 expression and IL-6 secretion[J].Proceedings of the National Academy of Sciences of the United States of America,2003,100(4):1751-1756.  
[47] BROUGHTON K S,BAYES J,CULVER B.High α-linolenic acid and fish oil ingestion promotes ovulation to the same extent in rats[J].Nutrition Research,2010,30(10):731-738.  
[48] SCHEIBEL S,DE OLIVEIRA C A L,DE ALVARENGA BOYD M,et al.DHA from microalgae Schizochytrium spp. (Thraustochytriaceae) modifies the inflammatory response and gonadal lipid profile in domestic cats[J].British Journal of Nutrition,2021,126(2):172-182.  
[49] JO Y,HAN S U,KIM Y J,et al.Suppressed gastric mucosal TGF-beta1 increases susceptibility to H. pylori-induced gastric inflammation and ulceration:a stupid host defense response[J].Gut and Liver,2010,4(1):43-53.  
[50] KOMPRDA T,SLÁDEK Z,ŠKULTÉTY O,et al.Effect of dietary Schizochytrium microalga oil on selected markers of low-grade inflammation in rats[J].Journal of Animal Physiology and Animal Nutrition,2016,100(6):1169-1178.  
[51] WANG X L,WANG H,PIERRE J F,et al.Marine microalgae bioengineered Schizochytrium sp. meal hydrolysates inhibits acute inflammation[J].Scientific Reports,2018,8(1):9848.
[52] TOMALUSKI C R,BAGGIO C,CAMPIGOTTO G,et al.Use of Schizochytrium spp. microalgae in suckling Holstein calves at different periods after birth[J].Livestock Science,2021,245:104424.
[53] MAVROMMATIS A,CHRONOPOULOU E G,SOTIRAKOGLOU K,et al.The impact of the dietary supplementation level with Schizochytrium sp, on the oxidative capacity of both goats' organism and milk[J].Livestock Science,2018,218:37-43.
[54] XIAO F F,XING J X,LI H D,et al.Effects of the defatted Schizochytrium sp. on growth performance,fatty acid composition,histomorphology and antioxidant status of juvenile mirror carp (Cyprinus carpio var. specularis)[J].Aquaculture Research,2021,52(7):3062-3076.  
[55] LONG S F,KANG S,WANG Q Q,et al.Dietary supplementation with DHA-rich microalgae improves performance,serum composition,carcass trait,antioxidant status,and fatty acid profile of broilers[J].Poultry Science,2018,97(6):1881-1890.  
[56] LV J W,YANG X Q,MA H X,et al.The oxidative stability of microalgae oil (Schizochytrium aggregatum) and its antioxidant activity after simulated gastrointestinal digestion:relationship with constituents[J].European Journal of Lipid Science and Technology,2015,117(12):1928-1939.  
[57] RYAN A S,KESKE M A,HOFFMAN J P,et al.Clinical overview of algal-docosahexaenoic acid:effects on triglyceride levels and other cardiovascular risk factors[J].American Journal of Therapeutics,2009,16(2):183-192.  
[58] SCORLETTI E,BYRNE C D.Omega-3 fatty acids,hepatic lipid metabolism,and nonalcoholic fatty liver disease[J].Annual Review of Nutrition,2013,33:231-248.
[59] TAI C C,DING S T.N-3 polyunsaturated fatty acids regulate lipid metabolism through several inflammation mediators:mechanisms and implications for obesity prevention[J].The Journal of Nutritional Biochemistry,2010,21(5):357-363.  
[60] YU J H,MA Y,SUN J,et al.Microalgal oil from Schizochytrium sp. prevents HFD-Induced abdominal fat accumulation in mice[J].Journal of the American College of Nutrition,2017,36(5):347-356.  
[61] MORI T A,BURKE V,PUDDEY I B,et al.Purified eicosapentaenoic and docosahexaenoic acids have differential effects on serum lipids and lipoproteins,LDL particle size,glucose,and insulin in mildly hyperlipidemic men[J].The American Journal of Clinical Nutrition,2000,71(5):1085-1094.  
[62] COLLA L M,MUCCILLO-BAISCH A L,VIEIRA COSTA J A.Spirulina platensis effects on the levels of total cholesterol,HDL and triacylglycerols in rabbits fed with a hypercholesterolemic diet[J].Brazilian Archives of Biology and Technology,2008,51(2):405-411.  
[63] GŁADKOWSKI W,KIEŁBOWICZ G,CHOJNACKA A,et al.The effect of feed supplementation with dietary sources of n-3 polyunsaturated fatty acids,flaxseed and algae Schizochytrium sp.,on their incorporation into lipid fractions of Japanese quail eggs[J].International Journal of Food Science & Technology,2014,49(8):1876-1885.  
[64] 陈秀丽,李连彬,岳洪源,等.裂殖壶菌粉对鸡蛋品质与蛋黄n-3PUFA含量的影响[J].中国畜牧杂志,2014,50(23):66-70. CHEN X L,LI L B,YUE H Y,et al.Effect of Schizochytrium limacinum on egg quality and content of n-3PUFA in egg yolk[J].Chinese Journal of Animal Science,2014,50(23):66-70.(in Chinese)
[65] 王浩,王晓翠,张海军,等.饲粮胆碱和裂殖壶菌油联合添加促进二十二碳六烯酸在鸡蛋卵黄中的富集[J].动物营养学报,2017,29(7):2374-2383. WANG H,WANG X C,ZHANG H J,et al.Dietary choline and Schizochytrium oil enhance docosahexaenoic acid enrichment in egg yolk[J].Chinese Journal of Animal Nutrition,2017,29(7):2374-2383.(in Chinese)
[66] 李浩洋,班甲,陈骏佳,等.日粮添加裂壶藻对鸡蛋DHA含量、品质及蛋鸡生产性能的影响[J].中国饲料,2015(11):37-39,42. LI H Y,BAN J,CHEN J J,et al.Effects of Schizochytrium supplemented in diets on DHA in eggs,egg quality and production performance of laying hens[J].China Feed,2015(11):37-39,42.(in Chinese)
[67] FRANKLIN S T,MARTIN K R,BAER R J,et al.Dietary marine algae (Schizochytrium sp.) increases concentrations of conjugated linoleic,docosahexaenoic and transvaccenic acids in milk of dairy cows[J].The Journal of Nutrition,1999,129(11):2048-2054.  
[68] MAVROMMATIS A,TSIPLAKOU E.The impact of the dietary supplementation level with Schizochytrium sp. on milk chemical composition and fatty acid profile,of both blood plasma and milk of goats[J].Small Ruminant Research,2020,193:106252.
[69] DE LIMA VALENÇA R,SILVA SOBRINHO A G D,BORGHI T H,et al.Performance,carcass traits,physicochemical properties and fatty acids composition of lamb's meat fed diets with marine microalgae meal (Schizochytrium sp.)[J].Livestock Science,2021,243:104387.
[70] MEALE S J,CHAVES A V,HE M L,et al.Dose-response of supplementing marine algae (Schizochytrium spp.) on production performance,fatty acid profiles,and wool parameters of growing lambs[J].Journal of Animal Science,2014,92(5):2202-2213.  
[71] 阿拉腾珠拉,刘文慧,马露等.裂壶藻和乳铁蛋白对大肠杆菌K99攻毒哺乳犊牛腹泻、生长性能、粪便评分及血清抗氧化指标的影响[J].动物营养学报,2020,32(9):4166-4176. ALATENGZHULA,LIU W H,MA L.Effects of Schizochytrium and lactoferrin on diarrhea,growth performance,fecal score and serum antioxidant indexes of lactating calves challenged with Escherichia coli K99[J].Journal of Animal Nutrition,2020,32(9):4166-4176.(in Chinese)
[72] SARDI L,MARTELLI G,LAMBERTINI L,et al.Effects of a dietary supplement of DHA-rich marine algae on Italian heavy pig production parameters[J].Livestock Science,2006,103(1/2):95-103.
[73] VOSSEN E,RAES K,VAN MULLEM D,et al.Production of docosahexaenoic acid (DHA) enriched loin and dry cured ham from pigs fed algae:nutritional and sensory quality[J].European Journal of Lipid Science and Technology,2017,119(5):1600144.
[74] KALBE C,PRIEPKE A,NVRNBERG G,et al.Effects of long-term microalgae supplementation on muscle microstructure,meat quality and fatty acid composition in growing pigs[J].Journal of Animal Physiology and Animal Nutrition,2019,103(2):574-582.  
[75] KIBRIA S,KIM I H.Impacts of dietary microalgae (Schizochytrium JB5) on growth performance,blood profiles,apparent total tract digestibility,and ileal nutrient digestibility in weaning pigs[J].Journal of the Science of Food and Agriculture,2019,99(13):6084-6088.  
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