Effects of Berberine-Oleanolate on Growth Performance, Serum Biochemical Indices and Intestinal Health of Piglets

BAO Xuetai; LI Yuying; TIAN Junquan; SU Wenxuan; YANG Fan; YAO Kang

doi:10.3969/j.issn.1006-267x.2022.03.014

Chinese Journal of Animal Nutrition >

2022 , Vol. 34 >Issue 3: 1495 - 1507

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.03.014

RESEARCH PAPER

Effects of Berberine-Oleanolate on Growth Performance, Serum Biochemical Indices and Intestinal Health of Piglets

BAO Xuetai ,
LI Yuying ,
TIAN Junquan ,
SU Wenxuan ,
YANG Fan ,
YAO Kang

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1. Hunan Provincial Engineering Research Center for Healthy Breeding of Livestock and Poultry, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China

Received date: 2021-07-29

Online published: 2022-03-14

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Abstract

This experiment was conducted to investigate the effects of different doses of dietary berberine-oleanolate on growth performance, serum biochemical indices and intestinal health of piglets infected with enterotoxigenic Escherichia coli (ETEC). Fifty 28-day-old healthy "Duroc×Landrace×Yorkshire" crossbred weaned piglets with body weight of (8.22±0.98) kg were randomly divided into 5 groups with 10 replicates per group and 1 pig per replicate fed in a single cage. Pigs in the control group and the model group were fed a basal diet, and those in the low, mid and high dose groups were fed the basal diet supplemented with 50, 250 and 500 mg/kg berberine-oleanolate, respectively. The pre-trial period lasted for 3 days and the trial period lasted for 18 days. On the 15^th day of the trial period, piglets in the model group, low, mid and high dose groups were given 10 mL ETEC K88 bacterial solution with a concentration of 2×10⁹ CFU/mL, and piglets in the control group were given the same dose of sterile LB culture solution. The results showed as follows:1) from days 1 to 14, that was, before ETEC K88 infection, the average daily feed intake (ADFI) of piglets in the mid dose group was significantly increased compared with the model group (P<0.05). From days 15 to 18, that was, after ETEC K88 infection, there were no significant differences in body weight, ADFI, average daily gain (ADG) and feed to gain ratio (F/G) among all groups (P>0.05). From days 1 to 18, compared with the model group, the ADFI of piglets in the mid dose group was significantly increased (P<0.05), and the F/G in high dose group was significantly higher than that in the model group and low dose group (P<0.05). 2) At 24 h after infection, the feces scoring of piglets in the model group was significantly increased compared with the control group (P<0.05); compared with the model group, the feces scoring of piglets in low, mid and high dose groups was decreased, but the difference was not significant (P>0.05). At 48 h after infection, the feces scoring of piglets in the model group was significantly higher than that in the control group (P<0.05); compared with the model group, the feces scoring of piglets in low, mid and high dose groups was significantly decreased (P<0.05). 3) Compared with the model group, the serum complement 4 content and the number of lymphocytes and eosinophils in blood of piglets in mid and high dose groups were significantly increased (P<0.05); compared with the control group, the blood platelet hematocrit in the model group was significantly decreased (P<0.05); the serum complement 3 content in the high dose group was significantly higher than that in the model group (P<0.05). 4) The crypt depth in jejunum of piglets in the model group was significantly higher than that in the other four groups (P<0.05); and the villus height in ileum in the mid and high dose groups was significantly higher than that in the control group, model group and low dose group (P<0.05). In conclusion, dietary berberine-oleanolate can improve the growth performance of weaned piglets, alleviate the diarrhea caused by ETEC K88 infection, reduce the effects of diarrhea on growth performance, intestinal health and serum biochemical indices of piglets, and improve the body immunity of piglets with diarrhea. Moreover, the supplemental dose of 500 mg/kg has a good therapeutic effect on intestinal injury of piglets infected with ETEC K88.

Key words： berberine-oleanolate; weaned piglets; growth performance; ETEC K88; diarrhea

Cite this article

BAO Xuetai , LI Yuying , TIAN Junquan , SU Wenxuan , YANG Fan , YAO Kang . Effects of Berberine-Oleanolate on Growth Performance, Serum Biochemical Indices and Intestinal Health of Piglets[J]. Chinese Journal of Animal Nutrition, 2022 , 34(3) : 1495 -1507 . DOI: 10.3969/j.issn.1006-267x.2022.03.014

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