Effects of Gallnut Extract on Growth Performance, Intestinal Morphology, Immune Function, Antioxidant Capacity and Intestinal Microbiota of Yellow-Feathered Chickens during 1 to 42 Days of Age

FAN Qiuli; CHEN Zhilong; LIN Zeling; ZHANG Sheng; MAO Shenli; JIANG Shouqun

doi:10.3969/j.issn.1006-267x.2022.03.018

2022 , Vol. 34 >Issue 3: 1547 - 1558

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.03.018

RESEARCH PAPER

Effects of Gallnut Extract on Growth Performance, Intestinal Morphology, Immune Function, Antioxidant Capacity and Intestinal Microbiota of Yellow-Feathered Chickens during 1 to 42 Days of Age

FAN Qiuli ,
CHEN Zhilong ,
LIN Zeling ,
ZHANG Sheng ,
MAO Shenli ,
JIANG Shouqun

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Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, State Key Laboratory of Livestock and Poultry Breeding, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China

Received date: 2021-08-12

Online published: 2022-03-14

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Abstract

The purpose of this study was to investigate the effects of gallnut extract on growth performance, intestinal morphology, immune function, antioxidant capacity and intestinal microbiota of yellow-feathered chickens during 1 to 42 days of age. A total of 600 healthy one-day-old female Xinguang 901 chickens with initial body weight of (39.75±0.02) g were randomly assigned to 4 groups with 6 replicates per group and 25 chickens per replicate. Chickens in the control group were fed a basal diet, chickens in the antibiotic group were fed the basal diet supplemented with 2 mg/kg enlanamycin, chickens in the gallnut 1 group were fed the basal diet supplemented with 250 mg/kg gallnut extract, and others in the gallnut 2 group were fed the basal diet supplemented with 500 mg/kg gallnut extract. The experiment lasted for 42 days. The results showed as follows:1) compared with the control group, the body weight at 42 days of age, average daily gain during 22 to 42 days of age and average daily gain during 1 to 42 days of age of gallnut 2 group were increased, but had no significantly difference (P>0.05). 2) Compared with the control group, the villus height and villus height/crypt depth value in ileum of gallnut 2 group were significantly increased (P<0.05); and the villus height/crypt depth value in ileum of gallnut 2 group was significantly higher than that of antibiotic group (P<0.05). 3) Compared with the control group, the contents of secretory immunoglobulin A (sIgA) and γ-interferon (IFN-γ) in jejunum of gallnut 1 group and gallnut 2 group were significantly increased (P<0.05), the contents of interleukin-8 (IL-8) and tumor necrosis factor-α (TNF-α) in jejunum were significantly decreased (P<0.05); and the jejunal sIgA content of gallnut 2 group was significantly higher than that of antibiotic group (P<0.05), the jejunal TNF-α content of gallnut 2 group was significantly lower than that of antibiotic group (P<0.05). 4) Compared with the control group, the ileal malondialdehyde (MDA) content of gallnut 1 group and gallnut 2 group was significantly decreased (P<0.05), and the ileal total superoxide dismutase (T-SOD) activity was significantly increased (P<0.05). 5) Compared with the control group, the mRNA relative expression levels of zonula occludens protein 1 (ZO-1) and nuclear factor-E2 related factor 2 (Nrf2) in ileum of gallnut 2 group were significantly increased (P<0.05); and the mRNA relative expression levels of ZO-1 and Nrf2 in ileum of gallnut 2 group were significantly higher than those of antibiotic group (P<0.05). 6) Compared with the control group, the relative abundances of Alistipes and Rikenella in cecum of gallnut 1 group and gallnut 2 group were significantly decreased (P<0.05), and the relative abundances of Bacillus and Lachnospira in cecum were significantly increased (P<0.05). In summary, dietary supplemented with 500 mg/kg gallnut extract can improve the growth performance, immune function and antioxidant capacity of yellow-feathered chickens during 1 to 42 days of age, improve the intestinal morphology, barrier function and microflora structure, it can be used as a potential substitute for antibiotics in chickens.

Key words： gallnut extract; yellow feathered chickens; immune function; antioxidant capacity; intestinal microbiota

Cite this article

FAN Qiuli , CHEN Zhilong , LIN Zeling , ZHANG Sheng , MAO Shenli , JIANG Shouqun . Effects of Gallnut Extract on Growth Performance, Intestinal Morphology, Immune Function, Antioxidant Capacity and Intestinal Microbiota of Yellow-Feathered Chickens during 1 to 42 Days of Age[J]. Chinese Journal of Animal Nutrition, 2022 , 34(3) : 1547 -1558 . DOI: 10.3969/j.issn.1006-267x.2022.03.018

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