RESEARCH PAPER

Effects of Nettle Cube Diet on Rumen Bacterial Community Structure, Fermentation Parameters and Growth Performance of Tibetan Sheep

  • ZHANG Xiaoqing ,
  • JIN Yanmei ,
  • YIN Fengxu ,
  • ZHANG Jize ,
  • WANG Zifan ,
  • PAN Qiang
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  • 1. Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot 010010, China;
    2. Institute of Practaculture Science, Tibet Academy of Agricultural and Animal husbandry Sciences, Lhasa 850000, China;
    3. Marine College, Shandong University, Weihai 264209, China;
    4. College of Agricultural and Biological Sciences, Dali University, Dali 671003, China;
    5. College of Grassland Science, Qingdao Agricultural University, Qingdao 266200, China

Received date: 2021-08-23

  Online published: 2022-03-14

Abstract

This experiment was conducted to study the effects of nettle cube diets on rumen bacterial community structure, fermentation parameters and growth performance of Tibetan sheep, and to provide theoretical basis and technical methods for the scientific application of nettle in Tibetan sheep production. Thirty 1-year-old Pengbo semi-fine wool rams with similar body weight were randomly assigned to three groups (n=10):free grazing group (group G, as control group), mixed nettle cube diet group (group M) and single nettle cube diet group (group N), respectively. The dietary concentrate to roughage ratios of groups M and N were 40:60 and 8:92, and the crude protein levels were 19.61% and 19.48%, respectively. The experimental period was 57 days, coinciding with a 18-day feed adaptation period and a 39-day formal period. The results showed as follows:1) the rumen bacterial operational taxonomic unit (OTU) number of groups M and N was significantly lower than that of groups G (P<0.05); the rumen bacterial ACE index, Chao1 index and Shannon index of groups M and N were significantly lower than those of group G (P<0.05), while the Simpson index was significantly higher than that of group G (P<0.05). 2) The relative abundances of Bacteroidetes and Prevotella_1, Prevotellaceae_UCG-003 in rumen of groups M and N were significantly higher than those of group G (P<0.05), while the relative abundances of Firmicutes, Ruminococcus_2 and Ruminococcaceae_NK4A214_group were significantly lower than those of group G (P<0.05). 3) The rumen pH of group N was significantly lower than that of groups G and M (P<0.05), the rumen total volatile fatty acid content of group M was significantly higher than that of groups G and N (P<0.05); the rumen propionic acid proportion of groups M and N was significantly higher than that of group G (P<0.05), while the rumen acetic acid/propionic acid and butyric acid proportion were significantly lower than those of group G (P<0.05). 4) The dry matter intake of group N was significantly lower than that of groups G and M (P<0.05); the average daily gain of groups M and N was significantly higher than that of group G (P<0.05), while the feed to gain ratio was significantly lower than that of group G (P<0.05). In conclusion, For the utilization of nettle in Tibetan sheep production, the single nettle cube diet is not beneficial to rumen fermentation; whereas the mixed nettle cube diet not only conform to the normal pattern of rumen fermentation, but also optimize the rumen bacterial community structure, consequently improve the growth performance of sheep. Therefore, the mixed nettle cube diet consisted of 60% Urtica tibetica plus 40% concentrate is more suitable for extensive application in local region.

Cite this article

ZHANG Xiaoqing , JIN Yanmei , YIN Fengxu , ZHANG Jize , WANG Zifan , PAN Qiang . Effects of Nettle Cube Diet on Rumen Bacterial Community Structure, Fermentation Parameters and Growth Performance of Tibetan Sheep[J]. Chinese Journal of Animal Nutrition, 2022 , 34(3) : 1683 -1693 . DOI: 10.3969/j.issn.1006-267x.2022.03.030

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