RESEARCH PAPER

Comparison and Analysis of Rumen Microflora Structure Difference between Hu Sheep and Karakul Sheep

  • CUI Haoran ,
  • BAI Tiantian ,
  • ZHAO Linbo ,
  • CHEN Yanxu ,
  • GUO Xuefeng ,
  • JIN Wei
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  • 1. College of Animal Science, Tarim University, Alar 843300, China;
    2. Key Laboratory of Tarim Animal Science and Technology Corps, Alar 843300, China;
    3. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210032, China

Received date: 2021-07-29

  Online published: 2022-03-14

Abstract

The purpose of this experiment was to use high-throughput sequencing method to study the differences in the structure of rumen microflora of Hu sheep and Karakul sheep fed the same diet. In the experiment, six healthy Karakul sheep and six Hu sheep with similar body weight[(35±2) kg] were selected and fed with the same diet. The experimental pre-feeding period was 30 d, and the formal feeding period was 3 d. The rumen fluid was collected from the oral cavity during the days 1 to 3 of the formal feeding period, and the 16S rRNA gene of the bacteria was sequenced and analyzed by high-throughput using PacBio sequencing technology. The results showed as follows:1) the diversity and richness of rumen microflora in Karakul sheep were significantly higher than those in Hu sheep (P<0.01). 2) The dominant bacteria phyla of Hu sheep and Karakul sheep were Firmicutes and Bacteroides. The relative abundance of Firmicutes in Hu sheep was significantly higher than that in Karakul sheep (P<0.05), and the relative abundance of Bacteroides was significantly lower than that in Karakul sheep (P<0.01). The dominant genera of Hu sheep were Quinnella, Butyrivibrio and Christensenaceae_R-7_group, and the dominant genus of Karakul sheep were Prevotella_1, Christensenaceae_R-7_group and Quinnella. The relative abundance of Quinnella in Hu sheep was significantly higher than that in Karakul sheep (P<0.01), and the relative abundance of Butyrivibrio was significantly higher than that in Karakul sheep (P<0.05); the relative abundances of Christensenaceae_R-7_group and Prevotella_1 in Karakul sheep were significantly higher than those in Hu sheep (P<0.01). The enrichments of biological metabolism pathways such as infectious diseases:bacterial, immune diseases, energy metabolism, folding, sorting and degradation in Hu sheep were higher than those in Karakul sheep, while the enrichments of biological metabolism pathways such as nucleotide metabolism, replication and repair, amino acid metabolism, carbohydrate metabolism and membrane transport in Karakul sheep were higher than that in Hu sheep. It is included that the rumen microflora structure of Hu sheep and Karakul sheep is similar, but there are some differences in the relative abundances of specific genera and the enrichments of some biological metabolism pathways. That may be one of the reasons for the better adaptability to high roughage diet of Karakul sheep.

Cite this article

CUI Haoran , BAI Tiantian , ZHAO Linbo , CHEN Yanxu , GUO Xuefeng , JIN Wei . Comparison and Analysis of Rumen Microflora Structure Difference between Hu Sheep and Karakul Sheep[J]. Chinese Journal of Animal Nutrition, 2022 , 34(3) : 1721 -1729 . DOI: 10.3969/j.issn.1006-267x.2022.03.033

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