RESEARCH PAPER

Effects of Bacillus amyloliquefaciens Isolated from Goats on Growth Performance, Slaughter Performance, Serum Biochemical and Immune Indexes, and Muscle Development-Related Gene Expression of Weanling Jintang Black Goat

  • ZHANG Nanchi ,
  • LI Juan ,
  • WANG Li ,
  • WEI Yong
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  • 1. Key Laboratory of Animal Science of State Ethnic Affairs Commission, Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Exploitation of Ministry of Education, Southwest Minzu University, Chengdu 610041, China;
    2. A'ba Vocational College, A'ba 623200, China;
    3. Animal Genetics and

Received date: 2020-07-23

  Online published: 2022-03-14

Abstract

The experiment was conducted to investigate the effects of Bacillus amyloliquefaciens isolated from goats on growth performance, slaughter performance, serum biochemical and immune indexes, and muscle development-related gene expression of weanling Jintang black goat. In this study, 20 weaned Jintang black goats were equally divided into two groups, and each had 10 goats. The experimental group was fed by 1 mL Bacillus amyloliquefaciens fsznc-06 with the concentration of 109 CFU/mL per 2 days. The control group was fed 1 mL cryoprotectant (3.102% glycerine, 2.926% sucrose and 7.096% glucose) per 2 days. The growth performance, slaughter performance, serum biochemical index, the expressions of muscle development and metabolism related genes were measured after a 30-day trial. The results showed that compared with the control group,the average daily gain, average daily feed intake, feed conversion ratio, carcass weight, dressing percentage, net meat weight, pure meat percentage, indexes of heart and liver of goats were significantly increased (P<0.05); total antioxidant capacity (T-AOC), immunoglobulin G (IgG) and interferon-γ (IFN-γ) contents in serum were significantly increased (P<0.05), and serum malonyldialdehyde (MDA) content was significantly decreased (P<0.05) after 30 days of feeding Bacillus amyloliquefaciens fsznc-06. The diameter and area of fiber of longissimus dorsi and gluteus were significantly decreased (P<0.05). The density of fiber of longissimus dorsi was significantly increased (P<0.05). The relative expression levels of fatty acid synthase (FAS), myogenic differentiation 1 (MYOD1), myogenin (MYOG), myosin heavy chain Ⅰ (MyHCⅠ) of longissimus dorsi and lipoprotein lipase (LPL), peroxisome proliferators-activated receptors-γ (PPAR-γ), MYOD1 and MyHCⅠ of gluteus were significantly increased (P<0.05). The relative expression levels of fibronectin 1 (FN1), transforming growth factor β1 (TGFβ1), sterol-regulatory element binding protein-1 (SREBP-1), myosin heavy chain Ⅱb (MyHCb), myosin heavy chain Ⅱx (MyHCx) of longissimus dorsi and FN1, TGFβ1, myosin heavy chain Ⅱa (MyHCa), MyHCb and MyHCx of gluteus were significantly decreased (P<0.05). In conclusion, Bacillus amyloliquefaciens fsznc-06 can effectively improve the growth performance, slaughter performance, immunological indexes and the relative expression levels of muscle development and metabolism related genes of Jintang black goat.

Cite this article

ZHANG Nanchi , LI Juan , WANG Li , WEI Yong . Effects of Bacillus amyloliquefaciens Isolated from Goats on Growth Performance, Slaughter Performance, Serum Biochemical and Immune Indexes, and Muscle Development-Related Gene Expression of Weanling Jintang Black Goat[J]. Chinese Journal of Animal Nutrition, 2022 , 34(3) : 1743 -1753 . DOI: 10.3969/j.issn.1006-267x.2022.03.035

References

[1] VAZQUEZ-MENDOZA P,ELGHANDOUR M M M,ALABA P A,et al.Antimicrobial and bactericidal impacts of Bacillus amyloliquefaciens CECT 5940 on fecal shedding of pathogenic bacteria in dairy calves and adult dogs[J].Microbial Pathogenesis,2018,114:458-463.
[2] ALGBURI A,ALAZZAWI S A,AL-EZZY A I A,et al.Potential probiotics Bacillus subtilis KATMIRA1933 and Bacillus amyloliquefaciens B-1895 co-aggregate with clinical isolates of Proteus mirabilis and prevent biofilm formation[J].Probiotics and Antimicrobial Proteins,2020,doi:10.1007/s12602-020-09631-0.
[3] LIU Y L,XING R G,YANG H Y,et al.Chitin extraction from shrimp (Litopenaeus vannamei) shells by successive two-step fermentation with Lactobacillus rhamnoides and Bacillus amyloliquefaciens[J].International Journal of Biological Macromolecules,2020,148:424-433.
[4] LIU C X,XU Q M,YU S C,et al.Bio-removal of tetracycline antibiotics under the consortium with probiotics Bacillus clausii T and Bacillus amyloliquefaciens producing biosurfactants[J].Science of the Total Environment,2020,710:136329.
[5] BINDIYA E S,TINA K J,SASIDHARAN R S,et al.BaCf3:highly thermostable bacteriocin from Bacillus amyloliquefaciens BTSS3 antagonistic on food-borne pathogens[J].Biotech,2019,9(4):136.
[6] FEI H,LIN G D,ZHENG C C,et al.Effects of Bacillus amyloliquefaciens and Yarrowia lipolytica lipase 2 on immunology and growth performance of Hybrid sturgeon[J].Fish & Shellfish Immunology,2018,82:250-257.
[7] WANG Y Y,WANG Y B,XU H,et al.Direct-fed glucose oxidase and its combination with B. amyloliquefaciens SC06 on growth performance,meat quality,intestinal barrier,antioxidative status,and immunity of yellow-feathered broilers[J].Poultry Science,2018,97(10):3540-3549.  
[8] 朱复跃.饲料中添加复合微生物制剂对肉鸭生长性能及粪便中NH3和H2S含量的影响[J].黑龙江畜牧兽医,2019(6):120-122. ZHU F Y.Effects of compound microorganism preparation in feed on growth performance and the content of NH3 and H2S of meat ducks[J].Heilongjiang Animal Science and Veterinary Medicine,2019(6):120-122.(in Chinese)
[9] 刘冬姣.饲料中添加复合微生物制剂对仔猪生长性能及粪便中NH3和H2S含量的影响[J].中国饲料,2018(21):17-20. LIU D J.Effects of compound microorganism preparation in feed on growth performance and the content of NH3 and H2S of piglet[J].China Feed,2018(21):17-20.(in Chinese)
[10] 王波,刁志成,马勇,等.日粮添加油脂和出生类型对羔羊肌肉胶原蛋白特性及相关基因表达的影响[J].畜牧兽医学报,2019,50(4):771-780. WANG B,DIAO Z C,MA Y,et al.Effects of dietary oil supplementation and birth types on muscle collagen characteristics and related genes expression in muscle of lamb[J].Chinese Journal of Animal and Veterinary Sciences,2019,50(4):771-780.(in Chinese)
[11] 史晓雪,牛占宇,幸超,等.梧桐籽油和罗格列酮对绵羊组织中脂代谢相关酶基因表达的影响[J].中国农业大学学报,2018,23(10):71-79. SHI X X,NIU Z Y,XING C,et al.Effects of phoenix tree seed oil and rosiglitazone on the expression of enzymes related to lipid metabolism of sheep tissues[J].Journal of China Agricultural University,2018,23(10):71-79.(in Chinese)
[12] 胡宇超,王园,孟子琪,等.发酵麸皮多糖对肉羊肉品质、肌肉氨基酸组成及肌肉抗氧化酶和肌纤维类型相关基因表达的影响[J].动物营养学报,2020,32(2):932-940. HU Y C,WANG Y,MENG Z Q,et al.Effects of fermented wheat bran polysaccharides on meat quality,muscle amino acid composition and expression of antioxidant enzymes and muscle fiber type-related genes in muscle of mutton sheep[J].Chinese Journal of Animal Nutrition,2020,32(2):932-940.(in Chinese)
[13] 尹丽卿.不同饲养方式下苏尼特羊肌纤维特性和MyHC基因对肉质的影响[D].硕士学位论文.呼和浩特:内蒙古农业大学,2016. YIN L Q.The effect of muscle fiber characteristics and MyHC gene on meat quality of Sunit sheep in different feeding conditions[D].Master's Thesis.Hohhot:Inner Mongolia Agricultural University,2016.(in Chinese)
[14] HÜTT P,SHCHEPETOVA J,LÕIVUKENE K,et al.Antagonistic activity of probiotic Lactobacilli and Bifidobacteria against entero- and uropathogens[J].Journal of Applied Microbiology,2006,100(6):1324-1332.  
[15] 郭政宏.藏猪源益生芽孢杆菌的分离鉴定及候选益生菌株的筛选[D].硕士学位论文.成都:西南民族大学,2016. GUO Z H.Isolation and identification of probiotic Bacillus from Tibetan pigs and screening of candidate probiotic strain[D].Master's Thesis.Chengdu:Southwest Minzu University,2016.(in Chinese)
[16] CHAUDHARI A A,LEE Y,LILLEHOJ H S.Beneficial effects of dietary supplementation of Bacillus strains on growth performance and gut health in chickens with mixed coccidiosis infection[J].Veterinary Parasitology,2020,277:109009.
[17] 杨艳红,余瑛,胡永强,等.解淀粉芽孢杆菌AF1对雏鸡益生性能和肠道菌群的影响[J].重庆理工大学学报(自然科学),2019,33(11):199-206. YANG Y H,YU Y,HU Y Q,et al.Effects of Bacillus amyloliquefaciens AF1 on chicken's live performance and intestinal flora[J].Journal of Chongqing University of Technology (Natural Science),2019,33(11):199-206.(in Chinese)
[18] 栾素军,邢焕,孙永波,等.解淀粉芽孢杆菌在畜禽养殖中的应用研究进展[J].中国畜牧兽医,2016,43(10):2615-2620. LUAN S J,XING H,SUN Y B,et al.Research Progress on Bacillus amyloliquefaciens in animal breeding Industry[J].China Animal Husbandry & Veterinary Medicine,2016,43(10):2615-2620.(in Chinese)
[19] 王美玲,吴骏晨,朱赟程,等.解淀粉芽孢杆菌在动物生产中应用的研究进展[J].中国畜牧杂志,2017,53(2):19-23. WANG M L,WU J C,ZHU C,et al.Research progress on application of Bacillus amyloliquefaciens in animal production[J].Chinese Journal of Animal Science,2017,53(2):19-23.(in Chinese)
[20] 李仕成.拮抗罗非鱼无乳链球菌的芽孢杆菌筛选与研究[D].硕士学位论文.长沙:湖南农业大学,2016. LI S C.Screening and studies of antagonistic Bacillus against Streptocossus agalactiae[D].Master's Thesis.Changsha:Hunan Agricultural University,2016.(in Chinese)
[21] 努孜古丽·图尔荪.新疆地方绵羊品种肌纤维特性与产肉量及肉品质的相关性研究[D].硕士学位论文.乌鲁木齐:新疆农业大学,2015. TUERSUN NUZIGULI.The correlation study to muscle fiber characteristics and meat quality with meat production of local sheep breeds in Xinjiang[D].Master's Thesis.Urumqi:Xinjiang Agricultural University,2015.(in Chinese)
[22] 杨敏馨,寇涛,李悦,等.解淀粉芽孢杆菌ES-2对肉鸡屠宰性能和肉品质及肌肉抗氧化能力的影响[J].南京农业大学学报,2016,39(2):255-261. YANG M X,KOU T,LI Y,et al.Effect of Bacillus amyloliquefaciens ES-2 on carcass characteristics,meat quality and antioxidative status of broilers[J].Journal of Nanjing Agricultural University,2016,39(2):255-261.(in Chinese)
[23] 张静,郭月英,任霆,等.生肌调节因子及其对肉质的影响[J].食品与发酵工业,2014,40(5):183-187. ZHANG J,GUO Y Y,REN T,et al.MRFs and the effect of MRFs on meat quality[J].Food and Fermentation Industries,2014,40(5):183-187.(in Chinese)
[24] BONNET M,CASSAR-MALEK I,CHILLIARD Y,et al.Ontogenesis of muscle and adipose tissues and their interactions in ruminants and other species[J].Animal,2010,4(7):1093-1109.  
[25] 赵广珍.MyoD1基因与猪肉质性状相关性分析[D].硕士学位论文.保定:河北农业大学,2012. ZHAO G Z.Relationship analysis between MyoD1 gene and meat quality traits in pig[J].Master's Thesis.Baoding:Hebei Agricultural University,2012.(in Chinese)
[26] 王薇.山猪肉质性状主要相关基因的遗传特性及其对肉质的作用和影响[D].博士学位论文.南京:南京师范大学,2012. WANG W.The genetic characteristics of the major genes related to quality traits of mountain pork and their effects on meat quality[D].Ph.D.Thesis.Nanjing:Nanjing Normal University,2012.(in Chinese)
[27] 王今越,王小虹,冯维斗.运动训练抑制了TGFβ通路并缓解了D-半乳糖诱导衰老大鼠的肌肉流失[J].体育科学,2014,34(10):72-77. WANG J Y,WANG X H,FENG W D.Exercise trainning inhibiting TGFβ pathway and improving D-glactose injection-induced senescent muscle loss in rat[J].China Sport Science,2014,34(10):72-77.(in Chinese)
[28] 栾兆进,刘开东,贺建宁,等.绵羊FAM134BPPARγHSLFAS基因表达量及与肌内脂肪含量的关系[J].畜牧兽医学报,2016,47(12):2379-2389. LUAN Z J,LIU K D,HE J N,et al.The FAM134B,PPARγ,HSL and FAS gene expression patterns and their association with intramuscular fat content in sheep[J].Chinese Journal of Animal and Veterinary Sciences,2016,47(12):2379-2389.(in Chinese)
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