RESEARCH PAPER

Effects of Arginine on Growth Performance, Body Composition, Plasma Biochemical Indexes and Antioxidant Capacity of Hybrid Snakehead(Channa maculata ♀×Channa argus ♂)

  • LI Peijia ,
  • CHEN Xiaoying ,
  • ZHAO Hongxia ,
  • CAO Junming ,
  • CHEN Bing ,
  • PENG Kai ,
  • HUANG Wen ,
  • GUO Hui
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  • 1. College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China;
    2. Guangdong Key Laboratory of Animal Breeding and Nutrition, Key Laboratory of Animal Nutrition and Feed Science in South China of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
    3. Guangzhou Fishtech Biotechnology Co., Ltd., Guangzhou 510640, China

Received date: 2021-08-04

  Online published: 2022-03-14

Abstract

The experiment was aimed to study the effects of dietary arginine on growth performance, body composition, plasma biochemical indexes and antioxidant capacity of juvenile hybrid snakehead (Channa maculata ♀×Channa argus ♂). A total of 750 hybrid snakeheads with an average initial weight of (22.02±0.02) g were selected and randomly divided into five groups with three replicates in each group and 50 fish in each replicate. Five experimental diets were prepared to contain arginine with 2.31%, 2.42%, 2.72%, 2.92% and 3.12%, respectively, recorded as G1, G2, G3, G4 and G5, respectively. The feeding period was 8 weeks. The results showed as follows:1) with the increase of dietary arginine level, weight gain rate, specific growth rate, protein productive rate of hybrid snakehead increased first and then decreased, and increased significantly in G4 group (P<0.05). Feed coefficient showed a downward trend and significantly decreased in G4 group (P<0.05). The level of arginine had no significant effects on survival rate, fattening degree, viserosomatic index of hybrid snakehead (P>0.05). 2) The contents of crude protein and ash in the whole fish of hybrid snakehead increased significantly in G4 group (P<0.05), but there was no significant difference in the contents of moisture and crude fat in the whole fish (P>0.05). With the increase of dietary arginine levels, arginine content in the whole fish of hybrid snakehead increased first and then decreased, and significantly increased in G4 group (P<0.05). The contents of leucine, methionine, valine, alanine, glycine, aspartic acid increased significantly in G3 group (P<0.05), and there was no significant difference in other amino acid contents (P>0.05). 3) The level of dietary arginine had no significant effect on plasma total protein, triglyceride, total cholesterol, urea nitrogen, glucose contents and glutamic oxalacetic transaminase and alanine transaminase activities (P>0.05). The activity of superoxide dismutase in G3 group was significantly higher than that in G1 and G2 groups (P<0.05), and the activity of glutathione peroxidase in G5 group was significantly higher than that in G2 group (P<0.05). Compared with G1 group, the total antioxidant capacity increased significantly (P<0.05) and the content of malonaldehyde decreased significantly in G5 group (P<0.05). In conclusion, the addition of appropriate level of arginine in the feed of hybrid snakehead significantly promote amino acid absorption, increase protein deposition, improve growth performance and antioxidant capacity. Under the experimental conditions, based on the specific growth rate, weight gain rate, protein productive rate indexes of hybrid snakehead, a comprehensive analysis through a broken line model shows that the optimal levels of arginine for hybrid snakehead juvenile fish are 2.91%, 2.95% and 2.98% (the feed protein contents are 6.63%, 6.65% and 6.72%).

Cite this article

LI Peijia , CHEN Xiaoying , ZHAO Hongxia , CAO Junming , CHEN Bing , PENG Kai , HUANG Wen , GUO Hui . Effects of Arginine on Growth Performance, Body Composition, Plasma Biochemical Indexes and Antioxidant Capacity of Hybrid Snakehead(Channa maculata ♀×Channa argus ♂)[J]. Chinese Journal of Animal Nutrition, 2022 , 34(3) : 1820 -1830 . DOI: 10.3969/j.issn.1006-267x.2022.03.042

References

[1] MASAGOUNDER K,HAYWARD R S,FIRMAN J D.Comparison of dietary essential amino acid requirements determined from group-housed versus individually-housed juvenile bluegill,Lepomis macrochirus[J].Aquaculture Nutrition,2011,17(2):e559-e571.
[2] 王连生,徐奇友.鱼类精氨酸营养研究进展[J].东北农业大学学报,2014,45(9):123-128. WANG L S,XU Q Y.Research advances in arginine nutrition of fish[J].Journal of Northeast Agricultural University,2014,45(9):123-128.(in Chinese)
[3] 梁明才,杨林.精氨酸抗氧化作用机制[J].生物信息学,2020,18(4):201-205. LIANG M C,YANG L.Antioxidant mechanism of arginine[J].China Journal of Bioinformatics,2020,18(4):201-205.(in Chinese)
[4] ZHENYAN C,DELBERT M,ALEJANDRO B.Dietary supplementation of arginine and/or glutamine influences growth performance, immune responses and intestinal morphology of hybrid striped bass (Morone chrysops×Morone saxatilis)[J].Aquaculture,2012,362/363:39-43.
[5] AHMED I.Dietary arginine requirement of fingerling Indian catfish (Heteropneustes fossilis,Bloch)[J].Aquaculture International,2013,21(2):255-271.  
[6] ZEHRA S,KHAN M A.Dietary arginine requirement of fingerling Indian major carp,Catla catla (Hamilton)[J].Journal of the World Aquaculture Society,2013,44(3):363-373.  
[7] ZHOU F,SHAO Q J,XIAO J X,et al.Effects of dietary arginine and lysine levels on growth performance,nutrient utilization and tissue biochemical profile of black sea bream, Acanthopagrus schlegelii,fingerlings[J].Aquaculture,2011,319(1/2):72-80.
[8] 曾雯娉,周歧存,王华朗,等.凡纳滨对虾精氨酸需要量的研究[C]//第八届世界华人虾蟹养殖学术研讨会.宁波:[s.n.],2012:164-165. ZENG W P,ZHOU Q C,WANG HUA L,et al.Study on arginine requirement of litopenaeus vannamei[C]//The 8th Symposium of World's Chinese Scientist's on Crustacean Aquaculture.Ningbo:[s.n.],2012:164-165.(in Chinese)
[9] 赵红霞,陈启明,黄燕华,等.饲料精氨酸水平对黄颡鱼幼鱼生长性能、消化吸收相关指标、免疫功能和抗氧化能力的影响[J].动物营养学报,2018,30(12):5040-5051. ZHAO H X,CHEN Q M,HUANG Y H,et al.Effects of dietary arginine level on growth performance,digestive and absorptive related indexes,immune function and antioxidant ability of juvenile yellow catfish (Pelteobagrus fulvidraco)[J].Chinese Journal of Animal Nutrition,2018,30(12):5040-5051.(in Chinese)
[10] 王连生,吴俊光,徐奇友,等.饲料中精氨酸水平对杂交鲟幼鱼肠道消化酶活性及形态结构的影响[J].大连海洋大学学报,2017,32(1):51-55. WANG L S,WU J G,XU Q Y,et al.Effects of dietary arginine levels on intestinal digestive enzyme activity and morphology in juvenile hybrid sturgeon[J].Journal of Dalian Fisheries University,2017,32(1):51-55.(in Chinese)
[11] CHEN G F,LIU Y,JIANG J,et al.Effect of dietary arginine on the immune response and gene expression in head kidney and spleen following infection of Jian carp with Aeromonas hydrophila[J].Fish & Shellfish Immunology,2015,44(1):195-202.  
[12] 赵红霞,曹俊明,吴建开,等.军曹鱼幼鱼对饲料中精氨酸的需要量[J].华南农业大学学报,2007(4):87-90. ZHAO H X,CAO J M,WU J K,et al.Studies of arginine requirement for juvenile Cobia[J].Journal of South China Agricultural University,2007(4):87-90.(in Chinese)
[13] 尹东鹏,陈秀梅,刘丹妮,等.乌鳢饲料赖氨酸及其它必需氨基酸营养需求量的研究[J].饲料工业,2018,39(14):18-23. YIN D P,CHEN X M,LIU D N,et al.Research on nutrient requirement of lysine and other essential amino acid in feed of juvenile Channa argus[J].Feed Industry,2018,39(14):18-23.(in Chinese)
[14] 陈启明.精氨酸对黄颡鱼生长、免疫及肠道健康的影响[D].硕士学位论文.广州:华南农业大学,2016. CHEN Q M.Effects of dietary arginine levels on growth,immunity and intestinal health of yellow catfish (Pelteobagrus fulvidraco)[D].Master's Thesis.Guangzhou:South China Agricultural University,2016.(in Chinese)
[15] 姜大丽,李治国,姜永杰,等.乌鳢营养需求研究进展[J].饲料工业,2021,42(4):29-35. JIANG D L,LI Z G,JIANG Y J,et al.Research advances in nutritional requirement of Channa argus[J].Feed Industry,2021,42(4):29-35.(in Chinese)
[16] POHLENZ C,BUENTELLO A,HELLAND S I J,et al.Effects of dietary arginine supplementation on growth,protein optimization and innate immune response of channel catfish Ictalurus punctatus (Rafinesque 1818)[J].Aquaculture Research,2014,45(3):491-500.  
[17] 韩凤禄,张琴,黄国强,等.斜带石斑鱼幼鱼的饲料精氨酸需求量[J].中国水产科学,2016,23(3):584-593. HAN F L,ZHANG Q,HUANG G Q,et al.Requirement of dietary arginine for juvenile orange-spotted grouper,Epinephelus coioides[J].Journal of Fishery Sciences of China,2016,23(3):584-593.(in Chinese)
[18] 代伟伟,麦康森,徐玮,等.饲料中赖氨酸和精氨酸含量对大菱鲆幼鱼生长、体成分和肌肉氨基酸含量的影响[J].水产学报.2015,39(6):12. DAI W W,MAI K S,XU W,et al.Effects of dietary lysine and arginine contents on growth,body composition and muscle amino acid contents of juvenile turbot[J].Journal of Fisheries of China,2015,39(6):12.(in Chinese)
[19] ZHOU F,ZHOU J,SHAO Q J,et al.Effects of arginine-deficient and replete diets on growth performance,digestive enzyme activities and genes expression of black sea bream,Acanthopagrus schlegelii,juveniles[J].Journal of the World Aquaculture Society,2012,43(6):828-839.  
[20] 廖英杰,刘波,任鸣春,等.精氨酸对团头鲂幼鱼生长、血清游离精氨酸和赖氨酸、血液生化及免疫指标的影响[J].中国水产科学,2014,21(3):549-559. LIAO Y J,LIU B,REN M C,et al.Effects of dietary arginine level on growth performance,free essential amino acids,hematological characteristics,and immune response in juvenile blunt snout bream (Megalobrama amblycephala)[J].Journal of Fishery Sciences of China,2014,21(3):549-559.(in Chinese)
[21] ALAM M S,TESHIMA S I,KOSHIO S,et al.Arginine requirement of juvenile Japanese flounder Paralichthys olivaceus estimated by growth and biochemical parameters[J].Aquaculture,2002,205(1/2):127-140.
[22] BERGE G E,BAKKE-MCKELLEP A M,LIED E.In vitro up take and interaction between arginine and lysine in the intestine of Atlantic salmon (Salmo salar)[J].Aquaculture,1999,179(1/2/3/4):181-193.
[23] 石桂城.脂肪对低温下吉富罗非鱼生化指标及脂肪酸组成的影响[D].硕士学位论文.湛江:广东海洋大学,2012. SHI G C.Effects of dietary lipid on the biochemical parameters and fatty acid composition of Gift under cold stress[D].Master's Thesis.Zhanjiang:Guangdong Ocean University,2012.
[24] CHEN H Y,LEU Y T,ROELANTS I.Quantification of arginine requirements of juvenile marine shrimp,Penaeus monodon,using microencapsulated arginine[J].Marine Biology,1992,114(2):229-233.  
[25] WINTERBOURN C C.Revisiting the reactions of superoxide with glutathione and other thiols[J].Archives of Biochemistry and Biophysics,2016,595:68-71.
[26] TRENZADO C E,MORALES A E,PALMA J M,et al.Blood antioxidant defenses and hematological adjustments in crowded/uncrowded rainbow trout (Oncorhynchus mykiss) fed on diets with different levels of antioxidant vitamins and HUFA[J].Comparative Biochemistry and Physiology. Toxicology & Pharmacology,2009,149(3):440-447.  
[27] 周婷婷,曹俊明,赵红霞,等.谷胱甘肽在水产动物中的营养生理作用研究进展[J].水产科学,2012,31(10):630-634. ZHOU T T,CAO J M,ZHAO H X,et al.Advances in nutritional and physiological effects of glutathione on aquatic animals[J].Fisheries Science,2012,31(10):630-634.(in Chinese)
[28] 李源,温安祥,骆美琳.谷氨酰胺促泥鳅生长机理的初步研究[J].饲料工业,2014,35(2):37-43. LI Y,WEN A X,LUO M L.A preliminary study of Misgurnus anguillicaudatus growth mechanism by glutamine[J].Feed Industry,2014,35(2):37-43.(in Chinese)
[29] KIR H M,DILLIOGLUGIL M O,TUGAY M,et al.Effects of vitamins E,A and D on MDA,GSH,NO levels and SOD activities in 5/6 nephrectomized rats[J].American Journal of Nephrology,2005,25(5):441-446.  
[30] BUENTELLO J A,REYES-BECERRIL M,DE JESÚ S ROMERO-GERALDO M,et al.Effects of dietary arginine on hematological parameters and innate immune function of channel catfish[J].Journal of Aquatic Animal Health,2007,19(3):195-203.  
[31] 吴俊光,王连生,王常安,等.饲料中精氨酸水平对杂交鲟幼鱼抗氧化能力及血清生化指标的影响[J].大连海洋大学学报,2016,31(3):272-279. WU J G,WANG L S,WANG C A,et al.Effects of dietary arginine levels on antioxidative state and serum biochemical indices in juvenile hybrid sturgeon[J].Journal of Dalian Fisheries University,2016,31(3):272-279.(in Chinese)
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