RESEARCH PAPER

Effects of Chinese Yam on Growth, Gut Defense Barrier and Intestinal Microflora of Common Carp (Cyprinus carpio L.)

  • LI Heng ,
  • CAO Hui ,
  • YANG Guokun ,
  • CHANG Xulu ,
  • ZHANG Yanmin ,
  • ZHANG Jianxin ,
  • HU Wenpan ,
  • ZHU Zhenxiang ,
  • LI Keke ,
  • MENG Xiaolin
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  • 1. College of Fisheries, Henan Normal University, Xinxiang 453007, China;
    2. Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation, Xinxiang 453007, China;
    3. Henan JinBaiHe Biotechnology Co., Ltd., Anyang 456150, China

Received date: 2021-08-18

  Online published: 2022-03-14

Abstract

To explore the effects of Chinese yam on growth, gut defense barrier and the intestinal microbiota of the common carp (Cyprinus carpio L.), a total of 180 carps with uniform individuals[(75.25±1.45) g] were allocated into 3 groups with 3 replicates of 20 fishes each, and fed a basal diet supplemented with 0 (control), 1% and 2% Chinese yam for 8 weeks. The changes of growth performance, intestinal enzyme activity, intestinal tissue morphology, intestinal defense barrier related gene expression, intestinal short chain fatty acids (SCFAs) content and intestinal microflora were detected. Results showed as follows:1)compared with the control group, the growth performance was not affected after adding the Chinese yam (P>0.05), but the intestinal villus density, number of goblet cells, number of intestinal mucosa folds and the height of intestinal villus in the treated groups increased, so as to improve the intestinal tissue structure and increase the absorption surface area. 2) Compared with the control group, the activities of catalase (CAT) and superoxide dismutase (SOD) were enhanced with the Chinese yam supplementation (P>0.05). The relative expression level of interleukin-1 β (IL-1β) gene was significantly increased (P<0.05), and the relative expression levels of transforming growth factor-β (TGF-β), Occludin, tight junction protein-1 (ZO-1) and Toll-like receptor 4 (TLR4) genes were also significantly up-regulated (P<0.05). 3)High throughput sequencing results revealed that the bacteria Fusobacteria, Proteobacteria and Bacteroidetes were the most abundant microbial community at the phylum level in the gut of the common carp. Compared with the control group, the abundance of Fusobacteria obviously increased (P<0.05), but the abundance of Proteobacteria decreased significantly in the Chinese yam treated groups (P<0.05). Also, at the genus level, the relative abundance of Cetobacterium significantly increased (P<0.05), accompanied with the relative abundance of Aeromonas significantly reduced after adding Chinese yam in the feed (P<0.05). 4)Compared with the control group, the contents of acetic acid and total SCFAs in the intestine of the common carp were also significantly increased (P<0.05) in the Chinese yam treated groups. These results suggest that Chinese yam supplemented feed can improve the immunity of the common carp by modulating the intestinal microflora and increase the gut defense barrier, and can be used as an immune-stimulating additive in aquaculture.

Cite this article

LI Heng , CAO Hui , YANG Guokun , CHANG Xulu , ZHANG Yanmin , ZHANG Jianxin , HU Wenpan , ZHU Zhenxiang , LI Keke , MENG Xiaolin . Effects of Chinese Yam on Growth, Gut Defense Barrier and Intestinal Microflora of Common Carp (Cyprinus carpio L.)[J]. Chinese Journal of Animal Nutrition, 2022 , 34(3) : 1831 -1844 . DOI: 10.3969/j.issn.1006-267x.2022.03.043

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