Effects of Peptide Supplemental Levels on Growth Performance, Immunity and Expression of Intestine Functional Genes in Sea Cucumber (Apostichopus japonicus)

LI Xiao; WANG Ying; JIANG Xiaodong; LI Hongyan; JI Lei; LIU Tianhong; SUN Yuanqin; LIU Hongjun

doi:10.3969/j.issn.1006-267x.2022.03.045

2022 , Vol. 34 >Issue 3: 1853 - 1863

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.03.045

RESEARCH PAPER

Effects of Peptide Supplemental Levels on Growth Performance, Immunity and Expression of Intestine Functional Genes in Sea Cucumber (Apostichopus japonicus)

LI Xiao ,
WANG Ying ,
JIANG Xiaodong ,
LI Hongyan ,
JI Lei ,
LIU Tianhong ,
SUN Yuanqin ,
LIU Hongjun

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1. Marine Science Research Institute of Shandong Province, Qingdao 266104, China;
2. Qingdao Quality Evaluation and Utilization Engineering Research Center for Aquatic Organism, Qingdao 266104, China;
3. Key Laboratory of Mariculture Disease Control of Shandong Province, Qingdao 266104, China

Received date: 2021-08-12

Online published: 2022-03-14

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Abstract

This experiment was conducted to determine the effects of peptide supplemental levels on growth performance, immunity and expression of intestine functional genes in sea cucumber (Apostichopus japonicas). A total of 300 sea cucumbers with an initial body weight of (34.40±0.23) g were selected, according to peptide supplemental levels (0, 5%, 10%, 15% and 20%), divided into 5 groups (D1, D2, D3, D4 and D5 groups), and each group had 3 replicates, each replicate had 20 sea cucumbers. The test was investigated the effects of polypeptides on growth performance and expression of intestinal functional genes (tendinogenic protein, collagen α2, integrin αV, integrin βL, p105, p50, rel and lys). The experiment lasted for 60 d. The results showed as follows:specific growth rate (SGR) of sea cucumbers in D4 group was significantly enhanced (P<0.05) and increased by 136% than that in D1 group; feeding rate (FR) was lower than that in other groups (P>0.05), and reduced by 25%. The antioxidant capacity (T-AOC), activities of superoxide capacity (SOD), acid phosphatase (ACP) and alkaline phosphatase (AKP) in coelomic fluid were increased with the increasing peptide supplement level. The expression levels of tenascin, collagen α2, integrin αV, intergrin βL genes in sea cucumbers in D2, D3 and D4 groups were significantly higher than those in the D1 group (P<0.05), and reached the peak in D4 group. The expression levels of p105, p50, rel and lys genes were increased with increasing amounts of peptide supplements. It is concluded that dietary 10% to 15% peptide can significantly increase the SGR of sea cucumber and decrease the FR. The expression of intestinal growth genes (tenascin gene, collagen α2 gene, integrin αV and intergrin βL) and immune genes (p105, p50, and lys) are up-regulated so as to promote the growth of sea cucumber and improve its immunity.

Key words： peptide; sea cucumber; growth; functional gene

Cite this article

LI Xiao , WANG Ying , JIANG Xiaodong , LI Hongyan , JI Lei , LIU Tianhong , SUN Yuanqin , LIU Hongjun . Effects of Peptide Supplemental Levels on Growth Performance, Immunity and Expression of Intestine Functional Genes in Sea Cucumber (Apostichopus japonicus)[J]. Chinese Journal of Animal Nutrition, 2022 , 34(3) : 1853 -1863 . DOI: 10.3969/j.issn.1006-267x.2022.03.045

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