Effects of High-Fat Diet on Feeding Rhythm and Expression of Hepatic Core Clock Proteins in Mice

LUO Jia; YAN Zheng; LI Shengtao; LU Zhuoheng; ZHANG Haoyue; XU Liping; WANG Xin

doi:10.3969/j.issn.1006-267x.2022.03.054

Chinese Journal of Animal Nutrition >

2022 , Vol. 34 >Issue 3: 1955 - 1962

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.03.054

RESEARCH PAPER

Effects of High-Fat Diet on Feeding Rhythm and Expression of Hepatic Core Clock Proteins in Mice

LUO Jia ,
YAN Zheng ,
LI Shengtao ,
LU Zhuoheng ,
ZHANG Haoyue ,
XU Liping ,
WANG Xin

Expand

School of Medicine, Ningbo University, Ningbo 315211, China

Received date: 2021-07-29

Online published: 2022-03-14

Fold

Abstract

This study was conducted to explore the effect of feeding rhythm on the metabolic syndrome (MS) induced by high-fat diet (HFD) using mouse model. Healthy male mice were divided into 4 groups with 5 mice per group, and mice in the 4 groups were fed a normal diet containing 4% fat (Con group) and three HFD containing 10% (10%HFD group), 45% (45%HFD group) and 60% (60%HFD group) fat for 3 months, respectively. The body weight was recorded every week. The serum and liver tissues were collected to detect biomarkers. In order to validate the effect of HFD on feeding rhythm, a second batch of mice were divided into Con group and 45%HFD group. In the first week, mice in the two groups were fed the normal diet. During the second and third weeks, mice in the Con group continue to fed the normal diet, but mice in the 45%HFD group were fed the HFD containing 45% fat. Diet consumption during night and day time was recorded for 3 days a week. Three weeks later, the mice were sacrificed and the liver tissues were collected for the analysis of expression changes of core clock proteins CLOCK and BMAL1. The results showed as follows:mice in the 45%HFD group gained the most body weight among all groups. Compared with the Con group, the contents of liver triglyceride (TG) and total cholesterol (TC) in the 45%HFD group were significantly decreased (P<0.05), the glucose tolerance showed abnormal phenomenon, and the pathological section showed liver steatosis. Compared with the Con group, the protein expression level of CLOCK in liver in the 10%HFD group, 45%HFD group and 60%HFD group was obviously decreased, while the mRNA expression levels of core clock genes Clock and Bmal1 had no significant differences among groups (P>0.05). Compared with the Con group, mice in the 45%HFD group increased daytime feed intake and increased nighttime feed intake, the diet consumption rhythm was inhibited. And again, the protein expression level of CLOCK in liver was significantly decreased by 45%HFD treatment (P<0.05), while the protein expression level of BMAL1 in liver had no significant change (P>0.05). In conclusion, HFD containing 45% fat is the strongest in inducing MS in mouse. Feeding rhythm is inhibited by HFD, which may be closely related to the down-regulation of liver CLOCK expression.

Key words： high-fat diet; feeding rhythms; core clock protein; metabolic syndrome

Cite this article

LUO Jia , YAN Zheng , LI Shengtao , LU Zhuoheng , ZHANG Haoyue , XU Liping , WANG Xin . Effects of High-Fat Diet on Feeding Rhythm and Expression of Hepatic Core Clock Proteins in Mice[J]. Chinese Journal of Animal Nutrition, 2022 , 34(3) : 1955 -1962 . DOI: 10.3969/j.issn.1006-267x.2022.03.054

References

[1] SAKLAYEN M G.The global epidemic of the metabolic syndrome[J].Current Hypertension Reports,2018,20(2):12.
[2] PEREIRA M A,KARTASHOV A I,EBBELING C B,et al.Fast-food habits,weight gain,and insulin resistance (the CARDIA study):15-year prospective analysis[J].Lancet,2005,365(9453):36-42.

[3] HUANG W Y,RAMSEY K M,MARCHEVA B,et al.Circadian rhythms,sleep,and metabolism[J].The Journal of Clinical Investigation,2011,121(6):2133-2141.

[4] ZIMMET P,ALBERTI K G M M,STERN N,et al.The circadian syndrome:is the metabolic syndrome and much more![J].Journal of Internal Medicine,2019,286(2):181-191.

[5] GEKAKIS N,STAKNIS D,NGUYEN H B,et al.Role of the CLOCK protein in the mammalian circadian mechanism[J].Science,1998,280(5369):1564-1569.

[6] LI D Y,IKAGA R,OGAWA H,et al.Different expressions of clock genes in fatty liver induced by high-sucrose and high-fat diets[J].Chronobiology International,2021,38(5):762-778.

[7] KOHSAKA A,LAPOSKY A D,RAMSEY K M,et al.High-fat diet disrupts behavioral and molecular circadian rhythms in mice[J].Cell Metabolism,2007,6(5):414-421.

[8] YANAGIHARA H,ANDO H,HAYASHI Y,et al.High-fat feeding exerts minimal effects on rhythmic mRNA expression of clock genes in mouse peripheral tissues[J].Chronobiology International,2006,23(5):905-914.

[9] LIAN C Y,ZHAI Z Z,LI Z F,et al.High fat diet-triggered non-alcoholic fatty liver disease:a review of proposed mechanisms[J].Chemico-Biological Interactions,2020,330:109199.
[10] JIANG P R,ZHENG W Y,SUN X N,et al.Sulfated polysaccharides from Undaria pinnatifida improved high fat diet-induced metabolic syndrome,gut microbiota dysbiosis and inflammation in BALB/c mice[J].International Journal of Biological Macromolecules,2021,167:1587-1597.
[11] MORENO-FERNÁNDEZ S,GARCÉS-RIMÓN M,VERA G,et al.High fat/high glucose diet induces metabolic syndrome in an experimental rat model[J].Nutrients,2018,10(10):1502.
[12] KHANNA S,WALIA S,KONDEPUDI K K,et al.Administration of indigenous probiotics modulate high-fat diet-induced metabolic syndrome in Sprague Dawley rats[J].Antonie van Leeuwenhoek,2020,113(9):1345-1359.

[13] POTTER G D M,CADE J E,GRANT P J,et al.Nutrition and the circadian system[J].The British Journal of Nutrition,2016,116(3):434-442.

[14] JANG H,LEE G,KONG J,et al.Feeding period restriction alters the expression of peripheral circadian rhythm genes without changing body weight in mice[J].PLoS One,2012,7(11):e49993.
[15] HATORI M,VOLLMERS C,ZARRINPAR A,et al.Time-restricted feeding without reducing caloric intake prevents metabolic diseases in mice fed a high-fat diet[J].Cell Metabolism,2012,15(6):848-860.

[16] PIVOVAROVA O,JVRCHOTT K,RUDOVICH N,et al.Changes of dietary fat and carbohydrate content alter central and peripheral clock in humans[J].The Journal of Clinical Endocrinology and Metabolism,2015,100(6):2291-2302.

[17] SHI D M,CHEN J,WANG J F,et al.Circadian clock genes in the metabolism of non-alcoholic fatty liver disease[J].Frontiers in Physiology,2019,10:423.
[18] TUREK F W,JOSHU C,KOHSAKA A,et al.Obesity and metabolic syndrome in circadian clock mutant mice[J].Science,2005,308(5724):1043-1045.

[19] LANDGRAF D,NEUMANN A M,OSTER H.Circadian clock-gastrointestinal peptide interaction in peripheral tissues and the brain[J].Best Practice & Research.Clinical Endocrinology & Metabolism,2017,31(6):561-571.

[20] STORCH K F,PAZ C,SIGNOROVITCH J,et al.Intrinsic circadian clock of the mammalian retina:importance for retinal processing of visual information[J].Cell,2007,130(4):730-741.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References