Effects of Boron on Microstructure, Hepatic Glycogen Content, Antioxidant Function, Cell Proliferation and Apoptosis Related Gene Expression in Liver of Rats

DING Yongwang; REN Man; ZHAO Chunfang; GU Youfang; JIN Erhui; LI Shenghe

doi:10.3969/j.issn.1006-267x.2022.03.055

2022 , Vol. 34 >Issue 3: 1963 - 1974

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.03.055

RESEARCH PAPER

Effects of Boron on Microstructure, Hepatic Glycogen Content, Antioxidant Function, Cell Proliferation and Apoptosis Related Gene Expression in Liver of Rats

DING Yongwang ,
REN Man ,
ZHAO Chunfang ,
GU Youfang ,
JIN Erhui ,
LI Shenghe

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1. College of Animal Science, Anhui Science and Technology University, Fengyang 233100, China;
2. Anhui Province Key Laboratory of Animal Nutritional Regulation and Health, Fengyang 233100, China

Received date: 2021-08-23

Online published: 2022-03-14

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Abstract

This experiment was conducted to study the effects of boron on microstructure, hepatic glycogen content, antioxidant function and of cell proliferation and apoptosis related gene expression in liver of rats. One hundred clean healthy (22±2)-day-old male SD rats were randomly divided into 10 groups with 10 rats per group after 7 days of adaptive feeding. The control group was drinking distilled water (boron content was 0), and the experimental groups were drinking distilled water which boron contents were 5, 10, 20, 40, 80, 160, 320, 480 and 640 mg/L, respectively. The experiment lasted for 60 days. The results showed as follows:1) compared with the control group, drinking water supplemented with 5, 10 and 20 mg/L boron could obviously improve the liver microstructure, the outline of hepatic lobule was clearer, the arrangement of hepatocyte cord was more orderly, the number of binuclear hepatocytes and Kupffer cells were increased, and then the liver microstructure was improved; while drinking water supplemented with 640 mg/L boron resulted in disordered arrangement of hepatocyte cord, broadening of hepatic sinusoid, deepening staining of hepatocyte nucleus and vacuole-like structure in hepatocyte. 2) Compared with the control group, drinking water supplemented with 5 and 10 mg/L boron significantly increased the content of hepatic glycogen in liver (P<0.01), significantly increased the superoxide dismutase (SOD) activity and total antioxidant capacity (T-AOC) in liver (P<0.05 or P<0.01), and significantly increased the mRNA relative expression levels of nuclear factor E2-related factor 2 (Nrf2) and proliferating cell nuclear antigen (PCNA) in liver (P<0.05). However, drinking water supplemented with 480 and 640 mg/L boron significantly decreased the content of hepatic glycogen in liver (P<0.01), significantly decreased the liver T-AOC (P<0.05 or P<0.01), significantly increased the liver malondialdehyde (MDA) content (P<0.05 or P<0.01), significantly increased the mRNA relative expression levels of B-cell lymphoma/leukaemia-2-associated X protein (Bax) and cysteinyl aspartate specific proteinase-3 (Caspase-3) in liver (P<0.01), and significantly decreased the mRNA relative expression levels of Nrf2, NAD(P)H:quinine oxidoreductase 1 (NQO1), PCNA and B-cell lymphoma/leukaemia-2 (Bcl-2) in liver (P<0.05 or P<0.01). In conclusion, drinking water supplemented with 5 and 10 mg/L boron can improve the liver microstructure of rats, increase the liver hepatic glycogen content and antioxidant capacity, and promote the liver Nrf2 and PCNA mRNA expression, while drinking water supplemented with 480 and 640 mg/L boron has the opposite effects.

Key words： boron; rats; liver; microstructure; antioxidant function; cell proliferation and apoptosis

Cite this article

DING Yongwang , REN Man , ZHAO Chunfang , GU Youfang , JIN Erhui , LI Shenghe . Effects of Boron on Microstructure, Hepatic Glycogen Content, Antioxidant Function, Cell Proliferation and Apoptosis Related Gene Expression in Liver of Rats[J]. Chinese Journal of Animal Nutrition, 2022 , 34(3) : 1963 -1974 . DOI: 10.3969/j.issn.1006-267x.2022.03.055

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