RESEARCH PAPER

Effects of Chronic Cold Exposure on Hepatic Antioxidant Function in Mice

  • LIU Yuanyuan ,
  • XUE Nianyu ,
  • ZHANG Boxi ,
  • LI Shize ,
  • LYU Hongming
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  • College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China

Received date: 2021-08-10

  Online published: 2022-03-14

Abstract

The purpose of this study was to investigate the effects of chronic cold exposure on the antioxidant function of mouse. Establishment of chronic cold exposure model in vivo:twelve 3-week-old C57BL/6 male mice were randomly divided into two groups (control group and cold exposure group) after feeding for one week, with 6 mice in each group. Mice in the cold exposure group were randomly placed at 4℃ for 3 h every day for 4 consecutive weeks. All mice were euthanized at the same time after cold exposure and their blood and liver were collected. In vitro cold exposure model establishment:mice liver cell line AML12 cells were randomly divided into 4 groups (control group, cold exposure group for 1 h, cold exposure group for 3 h, cold exposure group for 6 h). The cells of the cold exposure group were cultured in 37℃ incubator for 24 h, and then placed in 32℃ incubator for 0, 12, 24, 36 h, respectively. After the cold stimulation, the cells were scraped off for follow-up tests. The serum glutathione (GSH)and malondialdehyde (MDA) contents were detected by enzyme linked immunosorbent assay (ELISA). Western Blot was used to measure the expression of thioredoxin-1(Trx-1) and thioredoxin interacting protein (TXNIP) in mouse liver cells and tissues. The results showed that compared with the control group, the number of AML12 cells was significantly decreased after 36 h cold stimulation (P<0.05). Compared with the control group, the protein expression levels of Trx-1 was significantly decreased in both tissues and cells (P<0.05), the protein expression levels of TXNIP was significantly increased (P<0.05). ELISA showed that the content of GSH was significantly decreased (P<0.05) and the content of MDA in serum was significantly increased (P<0.05). In summary, mild low temperature treatment at 32℃ for a period of time can cause certain oxidative damage to mouse liver cells. After 4 weeks of chronic cold exposure at 4℃, the antioxidant function of mice decrease.

Cite this article

LIU Yuanyuan , XUE Nianyu , ZHANG Boxi , LI Shize , LYU Hongming . Effects of Chronic Cold Exposure on Hepatic Antioxidant Function in Mice[J]. Chinese Journal of Animal Nutrition, 2022 , 34(3) : 2023 -2029 . DOI: 10.3969/j.issn.1006-267x.2022.03.060

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