Research on Gut Microbial Diversity of Sows during Pregnancy and after Parturition

  • PANG Liyan ,
  • DING Hongxiang ,
  • XIAO Linli ,
  • LIU Yan ,
  • ZHENG Chen ,
  • FENG Tao
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  • 1. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
    2. Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China;
    3. Joint Laboratory of Animal Science between Institute of Animal Husbandry and Veterinary Medicine of Beijing Academy of Agriculture and Forestry Sciences and Division of Agricultural Science and Natural Resource of Oklahoma State University, Beijing 100097, China

Received date: 2021-09-25

  Online published: 2022-04-14

Abstract

This experiment was conducted to study the changes of gut microflora in sows at different stages during pregnancy and after delivery, and to analyze the relationship between intestinal microflora and maternal pregnancy physiology, in order to provide reference to maintain normal pregnancy. In this study, 16S rRNA high-throughput sequencing technology was used to reveal the gut microbial composition in 3 pregnancy stages (30, 70 and 110 d of pregnancy) and after delivery of healthy sows. The results showed that with the increase of gestational age, the gut microbial diversity of sows was decreased. The dominant phyla of gut microorganisms at 30, 70, 110 d of pregnancy and after delivery were Firmicutes and Bacteroidetes. At phylum level, the relative abundance of Spirochaetes was decreased with the progress of pregnancy, and the relative abundances of Proteobacteria, Actinobacteria and Fusobacteria and Patescibacteria were increased. At genus level, with the progress of pregnancy, the relative abundances of Prevotella, Porphyromona, Fusobacterium, Clostridium_sensu_stricto_1, Bacteroides and Escherichia-Shigella were increased, while the relative abundances of Lactobacillus and Treponema were decreased. The gut microbial community at 70 and 110 d of pregnancy was similar, but it was greatly different with that of 30 d of pregnancy and after delivery. All above indicated that microorganism development during pregnancy related with maternal metabolism enhancement, weight gain and fetal development in the middle and late pregnancy. In summary, the gut microflora of sows during pregnancy is dynamically changed. The diversity of gut microflora decreases and the abundance increases with the increase of gestational age, while the diversity and abundance decrease after delivery. The dominant bacterial phyla in the gut during pregnancy and after delivery are Firmicutes and Bacteroidetes, and the dominant bacterial genera are Clostridium_sensu_stricto_1, Lactobacillus and Bacteroides during pregnancy and Bacteroides, Escherichia-Shigella and Porphyromonas after delivery.

Cite this article

PANG Liyan , DING Hongxiang , XIAO Linli , LIU Yan , ZHENG Chen , FENG Tao . Research on Gut Microbial Diversity of Sows during Pregnancy and after Parturition[J]. Chinese Journal of Animal Nutrition, 2022 , 34(4) : 2208 -2220 . DOI: 10.3969/j.issn.1006-267x.2022.04.017

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