Effects of Compound Fiber and Plant Essential Oil Combined Supplementation on Growth Performance, Antioxidant Capacity and Immune Function of Weaned Piglets Challenged with Escherichia coli

  • LIANG Chan ,
  • CHEN Daiwen ,
  • HE Jun ,
  • ZHENG Ping ,
  • MAO Xiangbing ,
  • YU Jie ,
  • LUO Yuheng ,
  • LUO Junqiu ,
  • HUANG Zhiqing ,
  • YAN Hui ,
  • YU Bing
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  • Key Laboratory for Animal Disease-Resistance Nutrition of Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, China

Received date: 2021-08-26

  Online published: 2022-04-14

Abstract

This experiment was conducted to investigate the effects of compound fiber and plant essential oil combined supplementation on growth performance, antioxidant capacity and immune function of piglets challenged with enterotoxigenic Escherichia coli (ETEC). Twenty-eight healthy Duroc×Landrace×Yorkshire weaned piglets at (28±1) days of age were randomly divided into 4 groups according to the principle of similar body weight[(7.93±0.44) kg]. The 4 groups were control group (fed a basal diet), challenge control group (fed the basal diet), antibiotic group (fed the basal diet+75 mg/kg aureomycin+25 mg/kg virginiamycin+50 mg/kg quinocetone) and compound fiber and plant essential oil group (fed the basal diet+0.5% inulin+0.5% microcrystalline cellulose+200 mg/kg plant essential oil). There were 7 replicates in each group and 1 piglet in each replicate fed in a single cage. The experiment lasted for 25 days. On the morning of day 22, after fasting weighing, all pigs in the experimental groups were given 10 mL/kg BW Escherichia coli suspension (6×109 CFU/mL) at one time, while those in the control group were given normal saline in the same way. The results showed as follows:1) compared with the control group, the average daily gain (ADG) of piglets in the challenge control group was decreased by 7.19% (P>0.05), and the feed to gain ratio (F/G) was increased by 5.59% (P>0.05); the contents of malondialdehyde (MDA), interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in serum were significantly increased (P<0.05); the contents of immunoglobulin A (IgA) and interleukin-4 (IL-4) in serum were significantly decreased (P<0.05); and the MDA content and mRNA relative expression levels of interleukin-6 (IL-6), nuclear factor-κB p65 (NF-κB p65) and interleukin-1 receptor-associated kinase 1 (IRAK1) in jejunal mucosa were significantly increased (P<0.05). 2) Compared with the challenge control group, the average daily feed intake (ADFI) and ADG of piglets in the compound fiber and plant essential oil group were increased by 26.06% and 31.73%, respectively (P>0.05); the total antioxidant capacity (T-AOC) and contents of IgA and IL-4 in serum were significantly increased (P<0.05); the MDA content in serum and jejunal mucosa was significantly decreased (P<0.05); and the mRNA relative expression levels of IL-6, Toll-like receptor 4 (TLR4) and NF-κB p65 in jejunal mucosa were significantly decreased (P<0.05). 3) Compared with the antibiotic group, the serum IgA content in the compound fiber and plant essential oil group was significantly increased (P<0.05); and the mRNA relative expression levels of IL-1β, TNF-α, CD14, myeloid differentiation factor 88 (MyD88), NF-κB p65 and tumor necrosis factor receptor-related factor 6 (TRAF6) in jejunal mucosa were significantly decreased (P<0.05). In conclusion, the compound fiber and plant essential oil combined supplementation can significantly inhibit the oxidative stress and inflammatory response induced by Escherichia coli in piglets, and improve the growth performance of piglets, which has the potential to replace antibiotics.

Cite this article

LIANG Chan , CHEN Daiwen , HE Jun , ZHENG Ping , MAO Xiangbing , YU Jie , LUO Yuheng , LUO Junqiu , HUANG Zhiqing , YAN Hui , YU Bing . Effects of Compound Fiber and Plant Essential Oil Combined Supplementation on Growth Performance, Antioxidant Capacity and Immune Function of Weaned Piglets Challenged with Escherichia coli[J]. Chinese Journal of Animal Nutrition, 2022 , 34(4) : 2272 -2282 . DOI: 10.3969/j.issn.1006-267x.2022.04.023

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