Effects of Citrus Extract on Number of Microbes and Metabolites in Cecum of Piglets

CUI Yiyan; LU Huijie; LI Zhenming; TIAN Zhimei; YU Miao; DENG Dun; MA Xianyong

doi:10.3969/j.issn.1006-267x.2022.04.024

Chinese Journal of Animal Nutrition >

2022 , Vol. 34 >Issue 4: 2283 - 2291

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.04.024

Effects of Citrus Extract on Number of Microbes and Metabolites in Cecum of Piglets

CUI Yiyan ,
LU Huijie ,
LI Zhenming ,
TIAN Zhimei ,
YU Miao ,
DENG Dun ,
MA Xianyong

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1. Guangdong Engineering Technology Research Center of Animal Meat Quality and Safety Control and Evaluation, Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture, State Key Laboratory of Livestock and Poultry Breeding, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
2. Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Maoming 525000, China

Received date: 2021-09-27

Online published: 2022-04-14

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Abstract

This experiment aimed to investigate the effects of citrus extract on the number of microbes and metabolites in the cecal content of piglets. One hundred and forty-four 28-day-old Duroc×Landrace×Large White pigs with body weight of (8.39±1.06) kg were randomly assigned to 3 groups with 6 replicates per group and 8 pigs per pen (4 boars, 4 females). Piglets in the control group were fed a basal diet, piglets in the antibiotic group were fed the basal diet supplemented with 75 g/t chlortetracycline, and piglets in the citrus extract group were fed the basal diet supplemented with 300 mL/t citrus extract. The experiment lasted for 28 d. The results showed as follows:1) compared with control group, dietary citrus extract significantly increased the number of lactic acid bacteria in cecal content of piglets (P<0.05), while antibiotic significantly decreased the number of lactic acid bacteria (P<0.05). Compared with control group, dietary citrus extract and antibiotic significantly decreased the number of Escherichia coli in cecal content of piglets (P<0.05), and the number of Escherichia coli in citrus extract group was significantly lower than that in antibiotic group (P<0.05). 2) Compared with control and antibiotic groups, dietary citrus extract significantly increased the contents of taurine, threonine, serine, valine, leucine and lysine in cecal content of piglets (P<0.05). Compared with control group, dietary citrus extract significantly decreased the contents of aspartic acid, isoleucine, cystathionine, hydroxylysine, ornithine, proline and total amino acids in cecal content of piglets (P<0.05) and tended to decrease the contents of glutamate and alanine (0.05 ≤ P<0.10), and dietary antibiotic significantly decreased the content of ornithine and proline (P<0.05). 3) Compared with control group, dietary citrus extract significantly decreased the contents of phenol and skatole (P<0.05). Compared with control and antibiotic groups, dietary citrus extract significantly decreased the content of putrescine (P<0.05) and tended to decrease the contents of tyramine and spermine in cecal content of piglets (0.05 ≤ P<0.10). Compared with control group, dietary citrus extract and antibiotic significantly decreased the contents of methylamine in cecal content of piglets (P<0.05). In conclusion, citrus extract increases the number of lactic acid bacteria in cecal content of piglets, and decreases the contents of metabolites and the number of Escherichia coli.

Key words： citrus extract; piglets; biogenic amine; free amino acid; metabolite

Cite this article

CUI Yiyan , LU Huijie , LI Zhenming , TIAN Zhimei , YU Miao , DENG Dun , MA Xianyong . Effects of Citrus Extract on Number of Microbes and Metabolites in Cecum of Piglets[J]. Chinese Journal of Animal Nutrition, 2022 , 34(4) : 2283 -2291 . DOI: 10.3969/j.issn.1006-267x.2022.04.024

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