Changes of Gut Microbiota Structural Composition and Diversity of Bama-Mini Pigs during Pregnancy and Lactation Stage

MA Cui; GAO Qiankun; TANG Wu; ZHU Qian; ZHANG Wanghong; KONG Xiangfeng

doi:10.3969/j.issn.1006-267x.2022.04.025

Chinese Journal of Animal Nutrition >

2022 , Vol. 34 >Issue 4: 2292 - 2300

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.04.025

Changes of Gut Microbiota Structural Composition and Diversity of Bama-Mini Pigs during Pregnancy and Lactation Stage

MA Cui ,
GAO Qiankun ,
TANG Wu ,
ZHU Qian ,
ZHANG Wanghong ,
KONG Xiangfeng

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1. Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China

Received date: 2021-09-03

Online published: 2022-04-14

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Abstract

The present study was conducted to determine the changes of gut microbiota structural composition and diversity of Bama-mini pigs during pregnancy and lactation stage. Sixteen pregnant Bama-mini pigs with 3 to 7 parities were selected, and the fresh fecal samples were collected from sows on days 30, 60 and 90 of pregnancy and day 14 of lactation for fecal microbiota 16S rDNA sequencing. The results showed that compared with day 30 of pregnancy, the Simpson and Shannon indices of fecal microbiota on day 60 of pregnancy were significantly decreased (P<0.05), and the carbohydrate metabolic pathway and glycan biosynthesis and metabolic pathway relative abundances were significantly increased (P<0.05); the relative abundance of Turicibacter of fecal microbiota on day 14 of lactation was significantly decreased (P<0.05), and the Oscillospira and Dorea relative abundances were significantly increased (P<0.05). Compared with day 60 of pregnancy, the relative abundance of Lactobacillus of fecal microbiota on day 14 of lactation was significantly increased (P<0.05), and the carbohydrate metabolic pathway, glycan biosynthesis and metabolic pathway, and nucleotide metabolic pathway relative abundances were significantly decreased (P<0.05). Compared with day 90 of pregnancy, the Chao1 and ACE indices of fecal microbiota on day 14 of lactation were significantly decreased (P<0.05), the relative abundances of Firmicutes, Spirochaetes, Proteobacteria and Phascolarctobacterium were significantly increased (P<0.05), and the relative abundances of Bacteroidetes, Treponema and Roseburia were significantly decreased (P<0.05). Compared with days 30, 60 and 90 of pregnancy, the relative abundance of Cyanobacteria of fecal microbiota on day 14 of lactation was significantly decreased (P<0.05), and the Bulleidia relative abundance was significantly increased (P<0.05). In conclusion, the gut microbiota structural composition and diversity of Bama-mini pigs are change during different pregnancy and lactation stage, the relative abundances of fecal microbiota Bacteroides and Treponema of sows during mid pregnancy are higher, and the bacterial carbohydrate metabolism pathway and nucleotide metabolism pathway are up-regulated, to meet maternal nutritional needs and fetal growth and development needs.

Key words： Bama-mini pigs; pregnancy stage; lactation stage; gut microbiota; faece

Cite this article

MA Cui , GAO Qiankun , TANG Wu , ZHU Qian , ZHANG Wanghong , KONG Xiangfeng . Changes of Gut Microbiota Structural Composition and Diversity of Bama-Mini Pigs during Pregnancy and Lactation Stage[J]. Chinese Journal of Animal Nutrition, 2022 , 34(4) : 2292 -2300 . DOI: 10.3969/j.issn.1006-267x.2022.04.025

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