Effects of Bacillus subtilis on Growth Performance, Immune Performance and Serum Antioxidant Performance of Broilers Challenged with Lipopolysaccharide

  • YANG Minmin ,
  • CHE Yuyan ,
  • WANG Rui ,
  • WANG Haixin ,
  • LIU Bin ,
  • LI Yan ,
  • ZHANG Jiayi ,
  • QIN Shizhen ,
  • SHI Zhaoguo
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  • College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China

Received date: 2021-08-31

  Online published: 2022-04-14

Abstract

This experiment was aimed to investigate the effects of Bacillus subtilis (BS) on growth performance, immune performance and serum antioxidant indexes of broilers challenged with lipopolysaccharide (LPS). A total of 192 one-day-old healthy AA broilers (males and females in half) were randomly divided to 4 treatments as a 2×2 factorial design. The main factors were diets (a basic diet or a experimental diet supplemented with 200 g/t BS) and LPS stress (oral administration of LPS solution or saline), and randomly divided into 1) the basal diet+normal saline group; 2) the basal diet+LPS group; 3) the experimental diet+normal saline group; 4) the experimental diet+LPS group, a total 4 groups, each group composed of 6 replicates with 8 broilers per replicate. The trial lasted for 28 days and was divided into 3 stages, including before challenged with LPS (days 1 to 14), the period of challenged with LPS (days 15 to 21) and the period of recovery (days 22 to 28). The results showed as follows:1) before challenged with LPS, dietary BS significantly increased the average daily gain (ADG) of broilers (P< 0.05). LPS challenge significantly decreased ADG (P< 0.05) during the period of challenged with LPS and the period of recovery. The ADG of broilers with BS diet was significantly higher than that of broilers without BS diet (P< 0.05). During the period of recovery, dietary BS significantly decreased the feed conversion ratio (FCR) (P<0.05). 2) On day 21 and 28, under LPS stress, LPS challenged significantly increased the spleen index (P<0.05), dietary BS significantly decreased the spleen index on day 28 (P<0.05). During the whole period of experiment, dietary BS and LPS challenge had no significantly effects on thymus index and Bursa index (P>0.05). 3) On day 21, under LPS stress, LPS challenge significantly increased the content of immunoglobulin G (IgG) in serum of broilers (P<0.05), and dietary BS significantly decreased the content of immunoglobulin A (IgA) in serum of broilers (P<0.05). On day 28, there was no significantly effect on immunoglobulin contents in experimental groups (P>0.05).4) On day 21, compared with the basal diet, dietary BS had no effects on the contents of interleukin (IL)-1β、IL-6 and tumor necrosis factor-α (TNF-α) (P>0.05). LPS challenge significantly increased the contents of IL-1β, IL-6 (P<0.05). Under LPS stress, dietary BS significantly decreased the contents of IL-10 and TNF-α (P<0.05). On days 28, under LPS stress, dietary BS significantly decreased the contents of corticosteronr (CORT) and IL-1β (P<0.05). Compared with unstressed group, LPS challenge significantly increased the contents of IL-6 and TNF-α in serum (P<0.05),but had no effects on the content of IL-10 in serum (P>0.05). 5) On day 21, under LPS stress, dietary BS significantly increased the total antioxidant capacity (T-AOC), significantly decreased the content of malondialdehyde (MDA) in serum (P<0.05), and significantly increased the activity of catalase(CAT), but had no effects on the activity of superoxide dismutase (SOD) (P>0.05). On day 28, LPS challenge significantly decreased on the activity of SOD (P<0.05), and had no effect on T-AOC, MDA content and CAT activity in serum (P>0.05). In conclusion, dietary BS can alleviate the decrease of growth performance of broilers caused by immune stress, improve the serum antioxidant performance and inhibit the increase of serum inflammatory cytokine contents, and enhance the body immunity. Therefore, BS can reduce the damage of animal body caused with LPS challenge.

Cite this article

YANG Minmin , CHE Yuyan , WANG Rui , WANG Haixin , LIU Bin , LI Yan , ZHANG Jiayi , QIN Shizhen , SHI Zhaoguo . Effects of Bacillus subtilis on Growth Performance, Immune Performance and Serum Antioxidant Performance of Broilers Challenged with Lipopolysaccharide[J]. Chinese Journal of Animal Nutrition, 2022 , 34(4) : 2347 -2360 . DOI: 10.3969/j.issn.1006-267x.2022.04.030

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