Effects of Different Zinc Sources on Performance, Serum Biochemical Indexes and Microelement Deposition of Laying Hens

  • YANG Yuanting ,
  • HE Wanting ,
  • HUANG Liting ,
  • WANG Renkai ,
  • TIAN Yunbo ,
  • SHU Xugang
Expand
  • 1. College of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China;
    2. College of Chemistry and Chemical Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China

Received date: 2021-09-28

  Online published: 2022-04-14

Abstract

This experiment was conducted to study the effects of different zinc sources on the performance, egg quality, serum biochemical indexes and microelement deposition of laying hens. Jingfen-1 strain laying hens (n=288, 362-day-old) were randomly assigned to 4 groups with 6 replicates of 12 birds. Laying hens in control group were fed a basal diet supplemented with 80 mg/kg of zinc as zinc sulfate, and those in 3 experimental groups were fed the basal diet supplemented with equal amounts of zinc as glycinate (Gly-Zn), nano-zinc oxide (ZnO-NP) and zinc dihydromyricetin (DMY-Zn), respectively. The preparatory experiment period lasted for 14 days and the trial period lasted for 42 days. The results showed as follows:1) there was no significant difference in the performance of laying hens among groups (P>0.05). 2) Compared with the control group, the protein height and Haugh unit of Gly-Zn group and DMY-Zn group were significantly increased (P<0.05). 3) Compared with the control group, the triglyceride and low-density lipoprotein cholesterol contents in serum of DMY-Zn group were significantly reduced (P<0.05), the serum alkaline phosphatase activity of each trial group had an increasing trend (P=0.098), while the serum total cholesterol content had a decreasing trend (P=0.081). 4) Compared with the control group, the serum copper-zinc superoxide dismutase (CuZn-SOD) activity of ZnO-NP group, Gly-Zn group and DMY-Zn group was significantly increased (P<0.05), while the content of malondialdehyde (DMA) was significantly reduced (P<0.05). 5) Compared with the control group, the content of copper in liver and egg yolk of DMY-Zn group and ZnO-NP group was significantly increased (P<0.05), and the content of zinc in tibia was significantly increased (P<0.05). At the same time, the content of copper in tibia (P=0.078) and the content of zinc in egg yolk (P=0.088) in each trial group showed an increasing trend. 6) Compared with the control group, the content of copper in excrement of DMY-Zn group, Gly-Zn group and ZnO-NP group were significantly reduced (P<0.05), and the content of zinc in excrement of DMY-Zn group and ZnO-NP group were significantly reduced (P<0.05). In conclusion, there is little difference between DMY-Zn and ZnO-NP or Gly-Zn with 80 mg/kg zinc added to the basal diet of laying hens in improving egg quality, body antioxidant capacity and microelement deposition, but all of them are better than zinc sulfate; in addition, DMY-Zn also has the potential to improve the lipid metabolism of laying hens.

Cite this article

YANG Yuanting , HE Wanting , HUANG Liting , WANG Renkai , TIAN Yunbo , SHU Xugang . Effects of Different Zinc Sources on Performance, Serum Biochemical Indexes and Microelement Deposition of Laying Hens[J]. Chinese Journal of Animal Nutrition, 2022 , 34(4) : 2393 -2402 . DOI: 10.3969/j.issn.1006-267x.2022.04.034

References

[1] CUFADAR Y,GÖÇMEN R,KANBUR G,et al.Effects of dietary different levels of nano,organic and inorganic zinc sources on performance,eggshell quality,bone mechanical parameters and mineral contents of the tibia,liver,serum and excreta in laying hens[J].Biological Trace Element Research,2020,193(1):241-251.  
[2] HAN Q Q,GUO Y M,ZHANG B K,et al.Effects of dietary zinc on performance,zinc transporters expression,and immune response of aged laying hens[J].Biological Trace Element Research,2020,196(1):231-242.  
[3] TSAI Y H,MAO S Y,LI M Z,et al.Effects of nanosize zinc oxide on zinc retention,eggshell quality,immune response and serum parameters of aged laying hens[J].Animal Feed Science and Technology,2016,213:99-107.
[4] ABEDINI M,SHARIATMADARI F,KARIMI TORSHIZI M A,et al.Effects of zinc oxide nanoparticles on the egg quality,immune response,zinc retention,and blood parameters of laying hens in the late phase of production[J].Journal of Animal Physiology and Animal Nutrition,2018,102(3):736-745.  
[5] LI L L,GONG Y J,ZHAN H Q,et al.Effects of dietary Zn-methionine supplementation on the laying performance,egg quality,antioxidant capacity,and serum parameters of laying hens[J].Poultry Science,2019,98(2):923-931.  
[6] BEHJATIAN ESFAHANI M,MORAVEJ H,GHAFFARZADEH M,et al.Comparison the Zn-threonine,Zn-methionine,and Zn oxide on performance,egg quality,Zn bioavailability,and Zn content in egg and excreta of laying hens[J].Biological Trace Element Research,2021,199(1):292-304.  
[7] YU Q F,LIU H,YANG K L,et al.Effect of the level and source of supplementary dietary zinc on egg production,quality,and zinc content and on serum antioxidant parameters and zinc concentration in laying hens[J].Poultry Science,2020,99(11):6233-6238.  
[8] OLGUN O,YILDIZ A Ö.Effects of dietary supplementation of inorganic,organic or nano zinc forms on performance,eggshell quality,and bone characteristics in laying hens[J].Annals of Animal Science,2017,17(2):463-476.  
[9] GUO Z Y,CHEN X L,HUANG Z Q,et al.Dietary dihydromyricetin supplementation enhances antioxidant capacity and improves lipid metabolism in finishing pigs[J].Food & Function,2021,12(15):6925-6935.  
[10] CHANG Y C,YUAN L,LIU J R,et al.Dihydromyricetin attenuates Escherichia coli lipopolysaccharide-induced ileum injury in chickens by inhibiting NLRP3 inflammasome and TLR4/NF-κB signalling pathway[J].Veterinary Research,2020,51(1):72.
[11] 戴青.藤茶二氢杨梅素对肉鸡促生长作用研究[D].硕士学位论文.贵阳:贵州大学,2015. DAI Q.Study on the growth promoting effect of dihydromyricetin from ampelopsis grossedentata on chickens[D].Master’s Thesis.Guiyang:Guizhou University,2015.(in Chinese)
[12] HU H C,LUO F,WANG M J,et al.New method for extracting and purifying dihydromyricetin from Ampelopsis grossedentata[J].ACS Omega,2020,5(23):13955-13962.  
[13] WU C,ZHENG X P,CHEN L L.Study on antioxidant activity of dihydromyricetin-zinc(Ⅱ) complex[J].Advanced Materials Research,2011,183/185:863-867.
[14] 曾金华.二氢杨梅素-锌(Ⅱ)配合物的制备及其生物活性的研究[D].硕士学位论文.广州:广东工业大学,2014. ZENG J H.Study on the synthesis of dihydromyricetin-zinc (Ⅱ) complexes and biological activity[D].Master’s Thesis.Guangzhou:Guangdong University of Technology,2014.(in Chinese)
[15] 张慧.二氢杨梅素系列金属元素配合物的制备、稳定性及生物安全性研究[D].硕士学位论文.广州:广东工业大学,2019. ZHANG H.Preparation,stability and biosafety of metal complexes of dihydromyricetin[D].Master’s Thesis.Guangzhou:Guangdong University of Technology,2019.(in Chinese)
[16] LUO F,ZENG D D,WANG W X,et al.Bio-conditioning poly-dihydromyricetin zinc nanoparticles synthesis for advanced catalytic degradation and microbial inhibition[J/OL].Journal of Nanostructure in Chemistry,2021.[2021-09-12].https://doi.org/10.1007/s40097-021-00443-4.DOI:10.1007/s40097-021-00443-4.
[17] 初晖,张峰瑞,于庆港,等.L-肌肽对蛋鸡产蛋性能、蛋品质、养分表观消化率和血清生化指标的影响[J].动物营养学报,2021,33(10):5938-5946. CHU H,ZHANG F R,YU Q G,et al.Effects of L-carnosine on laying performance,egg quality,nutrient apparent digestibility and serum biochemical indices of laying hens[J].Chinese Journal of Animal Nutrition,2021,33(10):5938-5946.(in Chinese)
[18] ABD EL-HACK M E,ALAGAWANY M,ARIF M,et al.Organic or inorganic zinc in poultry nutrition:a review[J].World’s Poultry Science Journal,2017,73(4):904-915.
[19] QIN S Z,LU L,ZHANG X C,et al.An optimal dietary zinc level of brown-egg laying hens fed a corn-soybean meal diet[J].Biological Trace Element Research,2017,177(2):376-383.  
[20] OLECHNOWICZ J,TINKOV A,SKALNY A,et al.Zinc status is associated with inflammation,oxidative stress,lipid,and glucose metabolism[J].Journal of Physiological Sciences,2018,68(1):19-31.  
[21] ABD EL-HACK M E,ALAGAWANY M,SALAH A S,et al.Effects of dietary supplementation of zinc oxide and zinc methionine on layer performance,egg quality,and blood serum indices[J].Biological Trace Element Research,2018,184(2):456-462.  
[22] LIU D,MAO Y Q,DING L J,et al.Dihydromyricetin:a review on identification and quantification methods,biological activities,chemical stability,metabolism and approaches to enhance its bioavailability[J].Trends in Food Science & Technology,2019,91:586-597.
[23] 吴量,赵旭,邹田浩,等.蛋氨酸螯合锌替代硫酸锌对肉鸡生产性能、肉品质及抗氧化指标的影响[J].中国畜牧兽医,2020,47(4):1092-1098. WU L,ZHAO X,ZOU T H,et al.Effects of zinc methionine instead of zinc sulfate on growth performance,meat quality and antioxidant status of broilers[J].China Animal Husbandry & Veterinary Medicine,2020,47(4):1092-1098.(in Chinese)
[24] AKHAVAN-SALAMAT H,GHASEMI H A.Effect of different sources and contents of zinc on growth performance,carcass characteristics,humoral immunity and antioxidant status of broiler chickens exposed to high environmental temperatures[J].Livestock Science,2019,223:76-83.
[25] 陈娜娜,侯川川,马莲香,等.蛋氨酸锌对蛋鸡消化酶活性、蛋黄微量元素沉积、抗氧化及免疫指标的影响[J].中国畜牧杂志,2018,54(4):55-60. CHEN N N,HOU C C,MA L X,et al.Effects of zinc-methionine on digestive enzyme activity,yolk trace element accumulation,antioxidant capacity and serum immune indices of laying hens[J].Chinese Journal of Animal Science,2018,54(4):55-60.(in Chinese)
[26] 孙静静,张炳坤.家禽微量元素敏感指标研究进展[J].动物营养学报,2021,33(4):1841-1850. SUN J J,ZHANG B K.Research progress of trace element sensitive indexes for poultry[J].Chinese Journal of Animal Nutrition,2021,33(4):1841-1850.(in Chinese)
[27] ABEDINI M,SHARIATMADARI F,TORSHIZI M A K,et al.Effects of zinc oxide nanoparticles on performance,egg quality,tissue zinc content,bone parameters,and antioxidative status in laying hens[J].Biological Trace Element Research,2018,184(1):259-267.  
[28] 杜敏,杨玉艾,张晓敏,等.微量元素锌的研究进展[J].黑龙江畜牧兽医,2014(9):61-63. DU M,YANG Y A,ZHANG X M,et al.Research progress of trace element zinc[J].Heilongjiang Animal Science and Veterinary Medicine,2014(9):61-63.(in Chinese)
[29] 单安山.影响鸡羽毛中锌含量因素的探讨[J].畜牧兽医学报,1990(2):132-137. SHAN A S.Study on factors affecting zinc content in feather of chicken[J].Acta Veterinaria et Zootechnica Sinica,1990(2):132-137.(in Chinese)
Outlines

/