Effects of Mannose-Oligosaccharides on Rumen Fungal Flora of Tan Sheep Fed Buckwheat Straw Diet

  • MA Xiuhua ,
  • WEN Hongrui ,
  • MA Bucang ,
  • YANG Wanzong ,
  • ZHOU Yuxiang
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  • College of Agriculture, Ningxia University, Yinchuan 750021, China

Received date: 2021-09-16

  Online published: 2022-04-14

Abstract

In this experiment, internal transcribed spacer (ITS) sequencing technology was used to investigate the effects of adding 1%, 2% and 3% mannose oligosaccharides (MOS) on the rumen fungal flora of Tan sheep under the condition of buckwheat straw diet. Twenty healthy and disease-free weaned Ningxia Tan wethers with the body weight about 30.5 kg were randomly divided into 4 groups with 5 sheep in each group. Sheep in CK-3 group (control group) were fed a basal diet, sheep in Y1-3 group were fed the basal diet mixed with 1% MOS feeding, sheep in Y2-3 group were fed the basal diet mixed with 2% MOS, and sheep in Y3-3 group were fed the basal diet mixed with 3% MOS. The pre-feeding period was 15 days, and the normal feeding period was 60 days. The results showed as follows:1) the abundance and diversity of rumen fungal flora of Tan sheep in the Y1-3, Y2-3 and Y3-3 groups were obviously enhanced, and the effect of Y3-3 group was the most obvious. 2) At the phylum level, Ascomycota, Anthophyta and Basidiomycota were the dominant bacteria phyla shared by the four groups, but the differences in the relative abundances of them among the four groups were not significant (P>0.05). At the genus level, the relative abundance of Aspergillus in the Y-3 group was significantly higher than that in the other three groups (P<0.05). The abundance of disease prototype trophic type, saprophytic-pathogen-symbiotic trophic type, and symbiotic trophic type in the Y1-3 group was the least relative to other groups. In general, the addition of 3% MOS in the buckwheat straw diet under the experimental conditions is more conducive to increasing the diversity of rumen fungal flora of Tan sheep than other groups, and the rumen fungal flora of Tan sheep when diet supplemented with 1% MOS is more conducive to fiber degradation and rumen health.

Cite this article

MA Xiuhua , WEN Hongrui , MA Bucang , YANG Wanzong , ZHOU Yuxiang . Effects of Mannose-Oligosaccharides on Rumen Fungal Flora of Tan Sheep Fed Buckwheat Straw Diet[J]. Chinese Journal of Animal Nutrition, 2022 , 34(4) : 2514 -2523 . DOI: 10.3969/j.issn.1006-267x.2022.04.046

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