Effects of Colostrum Additives on Serum Indexes and Immunoglobulin G Absorption Efficiency of Newborn Lambs

TIAN Peizhi; TIAN Xingzhe; ZHAO Xiaoya; CHEN Jiaxin; SHI Chendi; YAN Hui; ZHANG Yingjie; JI Shoukun; LIU Yueqin

doi:10.3969/j.issn.1006-267x.2022.04.047

Chinese Journal of Animal Nutrition >

2022 , Vol. 34 >Issue 4: 2524 - 2533

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.04.047

Effects of Colostrum Additives on Serum Indexes and Immunoglobulin G Absorption Efficiency of Newborn Lambs

TIAN Peizhi ,
TIAN Xingzhe ,
ZHAO Xiaoya ,
CHEN Jiaxin ,
SHI Chendi ,
YAN Hui ,
ZHANG Yingjie ,
JI Shoukun ,
LIU Yueqin

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College of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, China

Received date: 2021-09-11

Online published: 2022-04-14

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Abstract

This experiment was conducted to investigate the effects of different colostrum additives on serum indexes and immunoglobulin G (IgG) absorption efficiency of newborn lambs. Sixty newborn health Hu lambs with the similar birth weight at (3.52±0.31) kg were selected and randomly divided into 6 groups with 10 lambs in each group, they were control group, antibiotic group, mannose oligosaccharide group, formic acid group, ethylenediamine tetraacetic acid (EDTA) group and cisapride group, respectively. Normal saline, antibiotics, mannose oligosaccharide, formic acid, EDTA and cisapride mixed with 100 mL of bovine colostrum were fed by oral intubation within 1 h of birth in above groups, respectively. Lambs were lactated with their mothers. Blood samples were collected from the jugular vein 24 h after birth. After centrifugation, serum samples were used to detect biochemical indexes, antioxidant indexes, inflammatory indexes, intestinal permeability indexes and immune indexes. Serum IgG contents from cattle and sheep were measured by single radial immunodiffusion to calculate the absorption efficiency of IgG. The results showed that serum glucose (GLU) content in antibiotic group and mannose oligosaccharide group was significantly higher than that in control group (P<0.05). Serum superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activities and total antioxidant capacity (T-AOC) in each experimental group were significantly lower than those in control group (P<0.05), and serum malondialdehyde (MDA) content was significantly higher than that in control group (P<0.05). Serum contents of interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) in each experimental group were significantly higher than those in control group (P<0.05). Serum lipopolysaccharide (LPS) content and diamine oxidase (DAO) activity in each experimental group were significantly higher than those in control group (P<0.05). IgG absorption efficiency in cisapride group and mannose oligosaccharide group was significantly lower than that in control group (P<0.05), which was 0 in formic acid group. Correlation analysis showed that IgG absorption efficiency of newborn lambs was positively correlated with serum SOD activity, T-AOC and IgA content (P<0.05), but negatively correlated with serum MDA, IL-1β and IL-6 contents (P<0.05). In conclusion, supplementations of antibiotics, mannose, formic acid, EDTA and cisapride in colostrum cannot improve the IgG absorption efficiency of newborn lambs; meanwhile, the results suggest that improving the serum antioxidant capacity and reducing the serum contents of inflammatory cytokines of newborn lambs may be effective measures to improve the IgG absorption efficiency.

Key words： newborn lambs; single radial immunodiffusion; IgG absorption efficiency; serum indexes

Cite this article

TIAN Peizhi , TIAN Xingzhe , ZHAO Xiaoya , CHEN Jiaxin , SHI Chendi , YAN Hui , ZHANG Yingjie , JI Shoukun , LIU Yueqin . Effects of Colostrum Additives on Serum Indexes and Immunoglobulin G Absorption Efficiency of Newborn Lambs[J]. Chinese Journal of Animal Nutrition, 2022 , 34(4) : 2524 -2533 . DOI: 10.3969/j.issn.1006-267x.2022.04.047

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