Effects of Mixing Ratio of Stems and Leaves of Whole Sugarcane (Sugarcane Tops) on Silage Quality and Aerobic Stability

GU Qichao; ZHANG Jie; ZHOU Xiaokang; ZHENG Yimin; ZOU Caixia

doi:10.3969/j.issn.1006-267x.2022.04.050

Chinese Journal of Animal Nutrition >

2022 , Vol. 34 >Issue 4: 2557 - 2574

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.04.050

Effects of Mixing Ratio of Stems and Leaves of Whole Sugarcane (Sugarcane Tops) on Silage Quality and Aerobic Stability

GU Qichao ,
ZHANG Jie ,
ZHOU Xiaokang ,
ZHENG Yimin ,
ZOU Caixia

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College of Animal Science and Technology, Guangxi University, Nanning 530000, China

Received date: 2021-08-31

Online published: 2022-04-14

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Abstract

In this experiment, the stems and leaves of the whole sugarcane (sugarcane tops) were separated and mixed according to different ratios to explore the influence on the silage quality, in order to improve the quality of sugarcane (sugarcane tops) silage quality and broaden the source of feed for ruminants. Experiment 1:after the sugarcane tops's stems and leaves were separated according to the sugarcane tops (ST1), 100% leaves+0 stems (100L1), 100% stems+0 leaves(100S1), 75% stems+25% leaves (75S1), 50% stems+50% stems (50S1), 25% stems+75% stems (25S1) were mixed separately; experiment 2:separate the whole sugarcane's stems and leaves according to the whole sugarcane (WS), 100% sugarcane tops+0 stems (ST2), 100% stems+0 sugarcane tops (100S2), 75% stems+25% sugarcane tops (75S2), 50% stems+50% sugarcane tops (50S2) and 25% stems+75% sugarcane tops (25S2) were mixed separately. Sampling and analysis after 215 days of silage. The results showed as follows:1) after silage, the content of crude fiber in ST1, 100L1 and 25S1 groups was significantly higher than that in other three groups (P<0.05); the contents of acetic acid, butyric acid and ethanol in 100S1 group were the highest; the soluble carbohydrate content in 50S1 group was significantly higher than that in other groups (P<0.05); stem proportion significantly affected the content of ammonia nitrogen in sugarcane tops silage (P<0.05), and 25S1 group was the highest. During the 1 to 4 days of aerobic exposure, the number of yeasts in the ST1 and 50S1 groups gradually decreased; during the 0 to 14 days of aerobic exposure, the number of Enterobacteria in the 75S1 group was relatively unchanged. 2) After silage, the silage pH in each groups was lower than 3.8; the contents of dry matter, ash and acid detergent fiber in the ST2 group were significantly higher than those in the other groups (P<0.05), and the contents of crude protein, hemicellulose and organic material were significantly lower than those in the other groups (P<0.001); crude fiber content in ST2 and 25S2 groups was significantly higher than that in other groups (P<0.05); Enterobacteria were not detected in WS, 100S2, 75S2 and 50S2 groups; acetic acid, butyric acid and ethanol contents in 100S2 group was the highest. During the 1 to 6 days of aerobic exposure, the number of Enterobacteria in the 50S2 group showed an increasing trend, while during the 6 to 14 days of aerobic exposure, the group showed a downward trend; during the 1 to 14 days of aerobic exposure, the number of Enterobacteria in the ST2 group showed a downward trend, the number of yeasts and mold in the ST2 and 25S2 groups showed a downward trend. In summary, after the stems and leaves of the whole sugarcane (sugarcane tops) are separated, the fermentation quality and aerobic stability of silage can be effectively improved by adjusting the stem content, and after the sugarcane tops's stems and leaves are separated, they are separated according to the sugarcane tops, 75% stems+25% leaves, 50% stems+50% stems, and the whole sugarcane's stems and leaves are separated according to the 100% sugarcane tops+0 stems, 50% stems+50% sugarcane tops and 25% stems+75% sugarcane tops respectively mixed and the silage is the best.

Key words： sugarcane tops; whole sugarcane; stems to leaves ratio; mixed silage; silage quality

Cite this article

GU Qichao , ZHANG Jie , ZHOU Xiaokang , ZHENG Yimin , ZOU Caixia . Effects of Mixing Ratio of Stems and Leaves of Whole Sugarcane (Sugarcane Tops) on Silage Quality and Aerobic Stability[J]. Chinese Journal of Animal Nutrition, 2022 , 34(4) : 2557 -2574 . DOI: 10.3969/j.issn.1006-267x.2022.04.050

References

[1] DA SILVA ÁVILA C L,VALERIANO A R,PINTO J C,et al.Chemical and microbiological characteristics of sugar cane silages treated with microbial inoculants[J].Revista Brasileira de Zootecnia,2010,39(1):25-32.

[2] 高雨飞,黎力之,欧阳克蕙,等.甘蔗梢作为饲料资源的开发与利用[J].饲料广角,2014(21):44-45. GAO Y F,LI L Z,OUYANG K H,et al.Development and utilization of sugarcane shoot as feed resources[J].Feed China,2014(21):44-45.(in Chinese)
[3] 覃方锉.添加剂对燕麦捆裹青贮品质的影响研究[D].硕士学位论文.兰州:甘肃农业大学,2014. QIN F C.Study on the effects of additives on baled silage quality of oat[D].Master’s Thesis.Lanzhou:Gansu Agricultural University,2014.(in Chinese)
[4] O’NEIL K A.Effect of microbial inoculants and sucrose on fermentation of alfalfa haylage[J].Journal of Animal Science,1986,63: 286-287.
[5] JONES B A,SATTER L D,MUCK R E.Influence of bacterial inoculant and substrate addition to lucerne ensiled at different dry matter contents[J].Grass and Forage Science,1992,47(1):19-27.

[6] DAVIES D R,MERRY R J,WILLIAMS A P,et al.Proteolysis during ensilage of forages varying in soluble sugar content[J].Journal of Dairy Science,1998,81(2):444-453.

[7] ADESOGAN A T,KRUEGER N,SALAWU M B,et al.The influence of treatment with dual purpose bacterial inoculants or soluble carbohydrates on the fermentation and aerobic stability of bermudagrass[J].Journal of Dairy Science,2004,87(10):3407-3416.

[8] 崔卫东,董朝霞,张建国,等.不同收割时间对甜玉米秸秆的营养价值和青贮发酵品质的影响[J].草业学报,2011,20(6):208-213. CUI W D,DONG Z X,ZHANG J G,et al.The nutrient components and ensilage fermentation quality of sweet corn stalks harvested at different times[J].Acta Prataculturae Sinica,2011,20(6):208-213.(in Chinese)
[9] GÓMEZ-VÁZQUEZ A,PINOS-RODRÍGUEZ J M,GARCÍA-LÓPEZ J C,et al.Nutritional value of sugarcane silage enriched with corn grain,urea,and minerals as feed supplement on growth performance of beef steers grazing stargrass[J].Tropical Animal Health and Production,2011,43(1):215-220.

[10] BAYTOK E,AKSU T,KARSLI M A,et al.The effects of formic acid,molasses and inoculant as silage additives on corn silage composition and ruminal fermentation characteristics in sheep[J].Turkish Journal of Veterinary and Animal Sciences,2005,29(2):469-474.
[11] NISHINO N,TOUNO T.Ensiling characteristics and aerobic stability of direct-cut and wilted grass silages inoculated with Lactobacillus casei or Lactobacillus buchneri[J].Journal of the Science of Food and Agriculture,2005,85(11):1882-1888.

[12] Association of Official Analytical Chemists.Official methods of analysis of the association of official analytical chemists[M].Washington,D.C.:Association of Official Analytical Chemists,1975.
[13] KRISHNAMOORTHY U,MUSCATO T V,SNIFFEN C J,et al.Nitrogen fractions in selected feedstuffs[J].Journal of Dairy Science,1982,65(2):217-225.

[14] VAN SOEST P J,ROBERTSON J B,LEWIS B A.Methods for dietary fiber,neutral detergent fiber,and nonstarch polysaccharides in relation to animal nutrition[J].Journal of Dairy Science,1991,74(10):3583-3597.

[15] ARTHUR T T.An automated procedure for the determination of soluble carbohydrates in herbage[J].Journal of the Science of Food and Agriculture,1977,28(7):639-642.

[16] PLAYNE M J,MCDONALD P.The buffering constituents of herbage and of silage[J].Journal of the Science of Food and Agriculture,1966,17(6):264-268.

[17] BRODERICK G A,KANG J H.Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media[J].Journal of Dairy Science,1980,63(1):64-75.

[18] 梁琼,鲁明波,卢正东,等.对羟基联苯法定量测定发酵液中的乳酸[J].食品科学,2008,29(6):357-360. LIANG Q,LU M B,LU Z D,et al.Determination of lactic acid in fermentation broth by p-hydroxybiphenol colorimetry[J].Food Science,2008,29(6):357-360.(in Chinese)
[19] ERWIN E S,MARCO G J,EMERY E M.Volatile fatty acid analyses of blood and rumen fluid by gas chromatography[J].Journal of Dairy Science,1961,44(9):1768-1771.

[20] COBLENTZ W K,MUCK R E.Effects of natural and simulated rainfall on indicators of ensilability and nutritive value for wilting alfalfa forages sampled before preservation as silage[J].Journal of dairy science,2012,95(11): 6635-6653.

[21] MUCK R E,O’KIELY P,WILSON R K.Buffering capacities in permanent pasture grasses [J].Irish Journal of Agricultural Research,1991,30(2):129-141.
[22] WEAVER D E,COPPOCK C E,LAKE G B,et al.Effect of maturation on composition and in vitro dry matter digestibility of corn plant parts[J].Journal of Dairy Science,1978,61(12):1782-1788.

[23] OLIVEIRA A S,WEINBERG Z G,OGUNADE I M,et al.Meta-analysis of effects of inoculation with homofermentative and facultative heterofermentative lactic acid bacteria on silage fermentation,aerobic stability,and the performance of dairy cows[J].Journal of Dairy Science,2017,100(6):4587-4603.

[24] SCHMIDT P,NUSSIO L G,ZOPOLLATTO M,et al.Chemical and biological additives in sugar cane silages:2.Ruminal parameters and DM and fiber degradabilities[J].Revista Brasileira de Zootecnia,2007,36(5):1676-1684.
[25] SHAO T,OHBA N,SHIMOJO M,et al.Effects of adding glucose,sorbic acid and pre-fermented juices on the fermentation quality of guineagrass (Panicum maximum Jacq.) silages[J].Asian-Australasian Journal of Animal Sciences,2004,17(6):808-813.

[26] 许留兴,熊康宁,杨苏茂,等.乳酸菌与蔗糖对金荞麦青贮品质研究[J].畜牧与兽医,2016,48(7): 54-59. XU L X,XIONG K N,YANG S M,et al.Study on the quality of Oyigoum cymosum silage with lactic acid bacteria and sucrose[J].Animal Husbandry ＆ Veterinary Medicine,2016,48(7):54-59.(in Chinese)
[27] LI M,ZI X J,ZHOU H L,et al.Effects of sucrose,glucose,molasses and cellulase on fermentation quality and in vitro gas production of king grass silage[J].Animal Feed Science and Technology,2014,197:206-212.
[28] MU L,XIE Z,HU L,et al.Lactobacillus plantarum and molasses alter dynamic chemical composition,microbial community,and aerobic stability of mixed (amaranth and rice straw) silage[J].Journal of the Science of Food and Agriculture,2021,101(12):5225-5235.

[29] 白春生,王莹,玉柱.添加剂对沙打旺青贮质量的影响[J].草原与草坪,2009(2):21-24,28. BAI C S,WANG Y,YU Z.Effects of different additives on silage quality of ereck milkvetch[J].Grassland and Turf,2009(2):21-24,28.(in Chinese)
[30] CHAMBERLAIN D G.Effect of added glucose and xylose on the fermentation of perennial ryegrass silage inoculated with Lactobacillus plantarum[J].Journal of the Science of Food and Agriculture,1989,46(2):129-138.

[31] YUNUS M,OHBA N,SHIMOJO M,et al.Effects of adding urea and molasses on napiergrass silage quality[J].Asian-Australasian Journal of Animal Sciences,2000,13(11):1542-1547.

[32] 李闯,陈冬灵,孙国明,等.青贮糖蔗和甜象草的营养成分对比分析[J].当代畜牧,2019(14):21. LI C,CHEN D L,SUN G M,et al.Comparative analysis of nutritional components between silage sugarcane and sweet elephant grass[J].Contemporary Animal Husbandry,2019(14):21.(in Chinese)
[33] KUNG L M,SHAVER R.Interpretation and use of silage fermentation analysis reports[J].Focus on forage,2001,3(13):1-5.
[34] 郑延平.怎样控制青贮饲料酸度过高[J].农村新技术,2011(24):63. ZHENG Y P.How to control the high acidity of silage[J].New Rural Technology,2011(24):63.(in Chinese)
[35] TRABI E B,YUAN X J,LI J F,et al.Effect of glucose and lactic acid bacteria on the fermentation quality,chemical compositions and in vitro digestibility of mulberry (Morus alba)leaf silage[J].Pakistan Journal of Zoology,2017,49(6):2271-2277.
[36] SHAO T,ZHANG L,SHIMOJO M,et al.Fermentation quality of Italian ryegrass (Lolium multiflorum Lam.) silages treated with encapsulated-glucose,glucose,sorbic acid and pre-fermented juices[J].Asian-Australasian Journal of Animal Sciences,2007,20(11):1699-1704.

[37] MAHANNA B,CHASE L E.Practical applications and solutions to silage problems[J].Silage Science and Technology,2003,42.
[38] DANIEL J L P,WEIB K,CUSTÓDIO L,et al.Occurrence of volatile organic compounds in sugarcane silages[J].Animal Feed Science and Technology,2013,185(1/2):101-105.
[39] ÁVILA C L S,BRAVO MARTINS C E C,SCHWAN R F.Identification and characterization of yeasts in sugarcane silages[J].Journal of Applied Microbiology,2010,109(5):1677-1686.
[40] DE FARIA PEDROSO A,NUSSIO L G,DE FÁTIMA PAZIANI S,et al.Fermentation and epiphytic microflora dynamics in sugar cane silage[J].Scientia Agricola,2005,62(5):427-432.

[41] MENARDO S,BALSARI P,TABACCO E,et al.Effect of conservation time and the addition of lactic acid bacteria on the biogas and methane production of corn stalk silage[J].Bioenergy Research,2015,8(4):1810-1823.

[42] HAM G A,STOCK R A,KLOPFENSTEIN T J,et al.Wet corn distillers byproducts compared with dried corn distillers grains with solubles as a source of protein and energy for ruminants[J].Journal of Animal Science,1994,72(12):3246-3257.

[43] JACOVACI F A,JOBIM C C,SCHMIDT P,et al.A data-analysis on the conservation and nutritive value of sugarcane silage treated with calcium oxide[J].Animal Feed Science and Technology,2017,225:1-7.
[44] BRUMM P J.Fermentation of single and mixed substrates by the parent and an acid-tolerant,mutant strain of Clostridium thermoaceticum[J].Biotechnology and Bioengineering,1988,32(4):444-450.

[45] ZHANG J G,KAWAMOTO H,CAI Y M.Relationships between the addition rates of cellulase or glucose and silage fermentation at different temperatures[J].Animal Science Journal,2010,81(3):325-330.

[46] WIERINGA G W.The influence of nitrate on silage fermentation[R].Wageningen:Wageningen University,1966.
[47] NILSSON G,NILSSON P E.The microflora on the surface of some fodder plants at different stages of maturity[J].Archiv fur Mikrobiologie,1956,24(4):412-422.

[48] DRIEHUIS F,WILKINSON J M,JIANG Y,et al.Silage review:animal and human health risks from silage[J].Journal of Dairy Science,2018,101(5):4093-4110.

[49] OGUNADE I M,JIANG Y,KIM D H,et al.Fate of Escherichia coli O157∶ H7 and bacterial diversity in corn silage contaminated with the pathogen and treated with chemical or microbial additives[J].Journal of Dairy Science,2017,100(3):1780-1794.

[50] LUO R B,ZHANG Y D,WANG F G,et al.Effects of sugar cane molasses addition on the fermentation quality,microbial community,and tastes of alfalfa silage[J].Animals,2021,11(2):355.
[51] WANG J,CHEN L,YUAN X J,et al.Effects of molasses on the fermentation characteristics of mixed silage prepared with rice straw,local vegetable by-products and alfalfa in southeast China[J].Journal of Integrative Agriculture,2017,16(3):664-670.

[52] DOLCI P,TABACCO E,COCOLIN L,et al.Microbial dynamics during aerobic exposure of corn silage stored under oxygen barrier or polyethylene films[J].Applied and Environmental Microbiology,2011,77(21):7499-7507.

[53] PAHLOW G,MUCK R E,DRIEHUIS F,et al.Microbiology of ensiling[J].Silage Science and Technology,2003,42:31-93.
[54] JONSSON A.Growth of Clostridium tyrobutyricum during fermentation and aerobic deterioration of grass silage[J].Journal of the Science of Food and Agriculture,1991,54(4):557-568.

[55] CARVALHO F,ÁVILA C L S,MIGUEL M G C P,et al.Aerobic stability of sugar-cane silage inoculated with tropical strains of lactic acid bacteria[J].Grass and Forage Science,2015,70(2):308-323.

[56] ROOSTITA R,FLEET G H.The occurrence and growth of yeasts in camembert and blue-veined cheeses[J].International Journal of Food Microbiology,1996,28(3):393-404.

[57] DUNIÈRE L,SINDOU J,CHAUCHEYRAS-DURAND F,et al.Silage processing and strategies to prevent persistence of undesirable microorganisms[J].Animal Feed Science and Technology,2013,182(1/2/3/4):1-15.

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