Effects of Lipopolysaccharide Challenge on Jejunal Morphology, Inflammatory Response and Necroptosis Signaling Pathway Related Gene Expression of Weaned Piglets

  • XU Qilong ,
  • WANG Yang ,
  • LI Xiangen ,
  • XIAO Kan ,
  • XIAO Shiping ,
  • WEI Weiqun ,
  • LIU Yulan
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  • 1. Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China;
    2. Jiangxi Tianjia Biological Engineering Company Limited, Nanchang 330200, China

Received date: 2021-09-03

  Online published: 2022-04-14

Abstract

The aim of this study was to investigate the effects of lipopolysaccharide (LPS) challenge on jejunal morphology, inflammatory response and necroptosis signaling pathway related gene expression of weaned piglets. Twenty weaned piglets with the average body of (7.1±0.9) kg and aged 28 days were randomly divided into 2 groups including control group and LPS challenge group, and each group had 6 piglets. Afer 9 day of preliminary feeding, the piglets in the LPS challenge group were treated with intraperitoneal injection of 100 μg/kg BW LPS, and the those in the control group were injected with the same amount of saline. Piglets were anesthetized for slaughter after 4 h post-injection, and the jejunum sample was taken for further test. The results showed as follows:compared with the control group, LPS challenge caused the jejunal villous atrophy and shedding, and significantly decreased the villous height and the crypt depth of piglets (P<0.05); LPS challenge significantly reduced the jejunal mucosa RNA/DNA (P<0.05), and had significant decreasing trend on protein and protein/DNA (P=0.054, P=0.095); LPS also significantly increased the mRNA relative expression levels of jejunal inflammatory factors including cyclooxygenase 2 (COX2), heat shock protein 70 (HSP70) and interleukin-6 (IL-6) (P<0.05); moreover, LPS significantly increased the mRNA relative expression levels of necroptosis signaling pathway related genes receptor including receptor-interacting protein kinase 1 (RIP1) and receptor-interacting protein kinase 3 (RIP3) (P<0.05). In conclusion, LPS challenge causes jejunal injury and inflammation, and activates the necroptosis signaling pathway of weaned piglets.

Cite this article

XU Qilong , WANG Yang , LI Xiangen , XIAO Kan , XIAO Shiping , WEI Weiqun , LIU Yulan . Effects of Lipopolysaccharide Challenge on Jejunal Morphology, Inflammatory Response and Necroptosis Signaling Pathway Related Gene Expression of Weaned Piglets[J]. Chinese Journal of Animal Nutrition, 2022 , 34(4) : 2606 -2612 . DOI: 10.3969/j.issn.1006-267x.2022.04.054

References

[1] LI H H,LI Y P,ZHU Q,et al.Dietary supplementation with Clostridium butyricum helps to improve the intestinal barrier function of weaned piglets challenged with enterotoxigenic Escherichia coli K88[J].Journal of Applied Microbiology,2018,125(4):964-975.  
[2] BLIKSLAGER A T,MOESER A J,GOOKIN J L,et al.Restoration of barrier function in injured intestinal mucosa[J].Physiological Reviews,2007,87(2):545-564.  
[3] 詹雅清,赖汉津,沈建通,等.程序性坏死的发生机制及其在缺血损伤性疾病中的研究进展[J].中华普通外科学文献(电子版),2021,15(3):224-228. ZHAN Y Q,LAI H J,SHEN J T,et al.Advances in the molecular mechanisms of necroptosis and its progression in organ ischemic injuries[J].Chinese Archives of General Surgery (Electronic Edition),2021,15(3):224-228.(in Chinese)
[4] LI Z,SCOTT M J,FAN E K,et al.Tissue damage negatively regulates LPS-induced macrophage necroptosis[J].Cell Death and Differentiation,2016,23(9):1428-1447.  
[5] DEGTEREV A,HUANG Z H,BOYCE M,et al.Chemical inhibitor of nonapoptotic cell death with therapeutic potential for ischemic brain injury[J].Nature Chemical Biology,2005,1(2):112-119.  
[6] 李先根.细胞程序性坏死在脂多糖诱导的仔猪肝脏损伤中的作用[D].硕士学位论文.武汉:武汉轻工大学,2019. LI X G.The role of necroptosis on lipopolysaccharide-induced liver injury in piglets [D].Master’s Thesis.Wuhan:Wuhan Polytechnic University,2019.(in Chinese)
[7] WANG Z C,FENG J N,YU J Y,et al.FKBP12 mediates necroptosis by initiating RIPK1-RIPK3-MLKL signal transduction in response to TNF receptor 1 ligation[J].Journal of Cell Science,2019,132(10):jcs227777.
[8] 黄菲菲,张阳,汪洋,等.脂多糖刺激后不同时间点断奶仔猪回肠和结肠中转化生长因子β1及Smads信号通路的动态变化[J].中国畜牧杂志,2020,56(12):104-108. HUANG F F,ZHANG Y,WANG Y,et al.Developmental changes of TGF-β1 and smads signaling pathway in ileum and colon of piglets at different timepoints after LPS challenge[J].Chinese Journal of Animal Science,2020,56(12):104-108.(in Chinese)
[9] 陈逢.鱼油通过TLR4和NOD信号通路对脂多糖诱导的仔猪肠道、肝脏损伤和肌肉蛋白质降解的调控作用[D].硕士学位论文.武汉:武汉轻工大学,2013. CHEN F.Regulative role of fish oil on intestinal and liver injury,and muscle protein degradation of piglets after lipopolysaccharide challenge through TLR4 and NOD signaling pathway[D].Master’s Thesis.Wuhan:Wuhan Polytechnic University,2013.(in Chinese)
[10] JOHNSON L R,CHANDLER A M.RNA and DNA of gastric and duodenal mucosa in antrectomized and gastrin-treated rats[J].The American Journal of Physiology,1973,224(4):937-940.  
[11] LIVAK K J,SCHMITTGEN T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt method[J].Methods,2001,25(4):402-408.  
[12] PI D A,LIU Y L,SHI H F,et al.Dietary supplementation of aspartate enhances intestinal integrity and energy status in weanling piglets after lipopolysaccharide challenge[J].The Journal of Nutritional Biochemistry,2014,25(4):456-462.  
[13] 朱惠玲,谢小利,刘玉兰,等.脂多糖应激对断奶仔猪肠黏膜免疫屏障的影响[J].中国畜牧杂志,2009,45(5):14-17. ZHU H L,XIE X L,LIU Y L,et al.Effect of lipopolysaccharide challenge on intestinal mucosal immune barrier in weanling pigs[J].Chinese Journal of Animal Science,2009,45(5):14-17.(in Chinese)
[14] CHEN Y X,XIE Y N,ZHONG R Q,et al.Effects of graded levels of xylo-oligosaccharides on growth performance,serum parameters,intestinal morphology,and intestinal barrier function in weaned piglets[J].Journal of Animal Science,2021,99(7):skab183.
[15] 刘坚,侯永清,丁斌鹰,等.α-酮戊二酸对脂多糖应激断奶仔猪空肠黏膜蛋白合成和抗氧化能力的影响[J].中国畜牧杂志,2010,46(11):35-38. LIU J,HOU Y Q,DING B Y,et al.Effects of α-ketoglutaric acid on protein synthesis and antioxidative capacity in jejunal mucosa of weaned pigs chronically challenged with lipopolysaccharide[J].Chinese Journal of Animal Science,2010,46(11):35-38.(in Chinese)
[16] LIU Y L,CHEN F,ODLE J,et al.Fish oil enhances intestinal integrity and inhibits TLR4 and NOD2 signaling pathways in weaned pigs after LPS challenge[J].The Journal of Nutrition,2012,142(11):2017-2024.  
[17] GLOBIG A M,HENNECKE N,MARTIN B,et al.Comprehensive intestinal T helper cell profiling reveals specific accumulation of IFN-γ+IL-17+coproducing CD4+ T cells in active inflammatory bowel disease[J].Inflammatory Bowel Diseases,2014,20(12):2321-2329.  
[18] 谢翔,应炜阳,金胜威.COX-2在炎症消退中的研究进展[J].生命的化学,2016,36(4):461-464. XIE X,YING W Y,JIN S W.Research progress of cyclooxygenase-2 in the resolution of inflammation[J].Chemistry of Life,2016,36(4):461-464.(in Chinese)
[19] 刘嘉敏,赵元莙.HSP70的研究进展及其在生物医学中的应用[J].教育教学论坛,2017(50):61-62. LIU J M,ZHAO Y J.Research progress for HSP70 and its application in biomedicine study[J].Education Forum,2017(50):61-62.(in Chinese)
[20] 梁婵华,黄妍,莫敏敏,等.元宝枫籽油改善脂多糖诱导的小鼠肠道炎症[J].现代食品科技,2021,37(10):37-45,6. LIANG C H,HUANG Y,MO M M,et al.Acer truncatum bunge seed oil attenuated the lipopolysaccharide-induced intestinal inflammation in mice[J].Modern Food Science & Technology,2021,37(10):37-45,6.(in Chinese)
[21] LANG C H,SILVIS C,DESHPANDE N,et al.Endotoxin stimulates in vivo expression of inflammatory cytokines tumor necrosis factor alpha,interleukin-1beta,-6,and high-mobility-group protein-1 in skeletal muscle[J].Shock,2003,19(6):538-546.  
[22] NEWTON K,MANNING G.Necroptosis and inflammation[J].Annual Review of Biochemistry,2016,85:743-763.
[23] 郭双,邢栋,吕勃.细胞凋亡及细胞程序性坏死和细胞焦亡的研究进展[J].中华实用诊断与治疗杂志,2021,35(3):321-324. GUO S,XING D,LYU B.Advances in apoptosis,necroptosis and pyroptosis[J].Journal of Chinese Practical Diagnosis and Therapy,2021,35(3):321-324.(in Chinese)
[24] HÄCKER H,KARIN M.Regulation and function of IKK and IKK-related kinases[J].Science’s STKE,2006,2006(357):re13.
[25] CHO Y S,CHALLA S,MOQUIN D,et al.Phosphorylation-driven assembly of the RIP1-RIP3 complex regulates programmed necrosis and virus-induced inflammation[J].Cell,2009,137(6):1112-1123.  
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