Comparative Study on Effects of Zinc Lactate and Zinc Sulfate on Zinc Transporter and Barrier Function in Pig Intestinal Epithelial Cells

HOU Ruoxin; LONG Jing; PENG Can; HE Liuqin; LI Tiejun; TANG Wenjie; KUANG Shengyao; YIN Yulong

doi:10.3969/j.issn.1006-267x.2022.04.056

2022 , Vol. 34 >Issue 4: 2626 - 2635

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.04.056

Comparative Study on Effects of Zinc Lactate and Zinc Sulfate on Zinc Transporter and Barrier Function in Pig Intestinal Epithelial Cells

HOU Ruoxin ,
LONG Jing ,
PENG Can ,
HE Liuqin ,
LI Tiejun ,
TANG Wenjie ,
KUANG Shengyao ,
YIN Yulong

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1. Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
2. College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100049, China;
3. Hunan Province Key Laboratory of Animal Intestinal Function and Regulation, College of Life Sciences, Hunan Normal University, Changsha 410081, China;
4. School of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
5. Sichuan Animal Feed Co., Ltd., Sichuan Key Laboratory of Livestock and Poultry Biological Products, Sichuan Key Laboratory of Animal Genetics and Breeding, Sichuan Animal Husbandry Research Institute, Chengdu 610066, China

Received date: 2021-08-25

Online published: 2022-04-14

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Abstract

The aim of this study was to compare the effects of zinc lactate and zinc sulfate on zinc transport function, physical barrier and immune barrier in intestinal epithelial cells IPEC-J2 of piglets. IPEC-J2 cells were cultured with 0, 1.0, 5.0, 7.5, 10.0 and 20.0 mg/mL zinc lactate or zinc sulfate (calculated by zinc) for 36 h, respectively, to screen out the optimal concentration of cell proliferation. Then the IPEC-J2 cells were randomly divided into control group, zinc lactate group and zinc sulfate group according to screening results, with 3 replicates in each group. The results showed as follows:1) compared with the control group, 7.5 mg/mL zinc lactate and zinc sulfate significantly increased the cell viability (P<0.05), and zinc lactate at the same concentration had a more significant promoting effect. 2) Compared with the control group, the contents of interleukin-1β (IL-1β) and interleukin-6 (IL-6) in cells in zinc lactate and zinc sulfate groups were significantly decreased (P<0.05), the tumor necrosis factor-α (TNF-α) content in cells in zinc sulfate group was significantly decreased (P<0.05), and the TNF-α mRNA relative expression level in cells in zinc lactate group was significantly decreased (P<0.05). 3) Compared with the control group, the occludin-1 protein relative expression level in cells in zinc lactate group was significantly increased (P<0.05), while the E-cadherin protein relative expression level in cells was not significantly different (P>0.05). 4) Compared with the control group, the contents of cysteine-rich intestinal protein-1 (CRIP1) and cysteine-rich intestinal protein 2 (CRIP2) in cells in zinc sulfate group were significantly increased (P<0.05), the mRNA relative expression levels of CRIP1 and CRIP2 in cells in zinc lactate group were significantly increased (P<0.05), and the mRNA relative expression level of zinc transporter 1 (ZNT-1) in cells in zinc lactate and zinc sulfate groups was significantly increased (P<0.05). In conclusion, zinc lactate and zinc sulfate can inhibit the secretion and expression of inflammatory cytokines in IPEC-J2 cells, promote the transport of zinc, enhance the intestinal tight junction, thus improve the intestinal immune function and mucosal structure integrity, improve the intestinal barrier function, and the effect of zinc lactate is better than that of zinc sulfate.

Key words： zinc lactate; IPEC-J2 cells; barrier function; immunity; zinc transporter

Cite this article

HOU Ruoxin , LONG Jing , PENG Can , HE Liuqin , LI Tiejun , TANG Wenjie , KUANG Shengyao , YIN Yulong . Comparative Study on Effects of Zinc Lactate and Zinc Sulfate on Zinc Transporter and Barrier Function in Pig Intestinal Epithelial Cells[J]. Chinese Journal of Animal Nutrition, 2022 , 34(4) : 2626 -2635 . DOI: 10.3969/j.issn.1006-267x.2022.04.056

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