Alleviative Effect of Vitamin A on Milk Fat Synthesis Reduction in Bovine Mammary Epithelial Cells Injured by Nitric Oxide

  • ZHAO Yanli ,
  • LIU Jintao ,
  • LI Yuan ,
  • GUO Xiaoyu ,
  • YAN Sumei ,
  • GUO Yongmei ,
  • ZHENG Yaguang
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  • Key Laboratory of Animal Nutrition and Feed Science at Universities of Inner Mongolia Autonomous Region, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2021-12-15

  Online published: 2022-04-14

Abstract

This study was conducted to investigate the alleviative effect of vitamin A (VA) on the enzyme activities and gene expression relate to milk fat synthesis in bovine mammary epithelial cells (BMECs) injured by nitric oxide (NO), and to screen the optimum supplemental level of VA. The single-factor completely randomized trial design was used in this study. The diethylenetriamine/nitric oxide adduct (DETA/NO) was used to injure cells. The control group cells were cultured without VA and DETA/NO for 30 h. The test group 1 (named as NO group) was treated without VA for 24 h and added DETA/NO for another 6 h. The test groups 2 to 8 were treated with 0.05, 0.10, 0.20, 0.50, 1.00, 2.00 and 4.00 μg/mL VA for 24 h and added DETA/NO for another 6 h, they were named NOA0.05, NOA0.1, NOA0.2, NOA0.5, NOA1, NOA2 and NOA4 groups. Each group had 6 replicates. The results showed that, compared with the control group, the cell viability, the activities of glutathione peroxidase (GPx), catalase (CAT), thioredoxin (TrxR), fatty acid synthetase (FASN), lipoprotein lipase (LPL), acetyl-CoA carboxylase (ACC) and stearyl CoA desaturase (SCD), the content of triglyceride (TG), total antioxidant capacity (T-AOC) and the mRNA relative expression levels of GPx, TrxR, peroxidase proliferators activate the receptor γ (PPARγ), sterol regulatory element binding protein 1 (SREBP1), ACC and FASN of the NO group were significantly decreased (P<0.05), but the ROS and iNOS activities and NO content were significantly increased (P<0.05). Compared with the NO group, the cell viability, T-AOC, the activities of ACC, FASN, LPL, and the mRNA relative expression levels of GPx1, TrxR, FASN, LPL, PPARγ and SREBP1 of the NOA0.1 to NOA4 groups were significantly increased (P<0.05); the TG content, the activities of TrxR, CAT, GPx and SCD, and the SCD mRNA relative expression level of the NOA0.2 to NOA2 groups were significantly increased (P<0.05), while the iNOS activity and NO content were significantly decreased (P<0.05). These results indicate that BMECs injury induced by NO result the reduction of antioxidant function, milk fat synthesis related enzyme activities and gene expression and TG synthesis. VA can alleviate the reduction of antioxidant function and milk fat synthesis, and 0.20 to 2.00 μg/mL VA have better effect, especially 1.00 μg/mL VA has the best effect.

Cite this article

ZHAO Yanli , LIU Jintao , LI Yuan , GUO Xiaoyu , YAN Sumei , GUO Yongmei , ZHENG Yaguang . Alleviative Effect of Vitamin A on Milk Fat Synthesis Reduction in Bovine Mammary Epithelial Cells Injured by Nitric Oxide[J]. Chinese Journal of Animal Nutrition, 2022 , 34(4) : 2636 -2644 . DOI: 10.3969/j.issn.1006-267x.2022.04.057

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