Effects of Hesperidin and Rosmarinic Acid Combination on Ileal Morphology, Microbiota Structure and Inflammatory Response of Lipopolysaccharide-Challenged Rats

  • LI Panpan ,
  • LI Runlin ,
  • WU Jiaqing ,
  • LEI Mingkang ,
  • WANG Jing ,
  • ZHU Weiyun
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  • 1. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China;
    2. National Center for International Research on Animal Gut Nutrition, Nanjing 210095, China

Received date: 2021-09-16

  Online published: 2022-04-14

Abstract

This study was conducted to explore the effects of hesperidin and rosmarinic acid combination on ileal morphology, microbiota structure and inflammation response of rats challenged with lipopolysaccharide (LPS). Thirty-two specific-pathogen-free (SPF) Sprague-Dawley (SD) rats aged 21 days were randomly divided into four groups:LPS group, Hes group, RA group, Hes×RA group, and each group consisted of four replicates with two rats per replicate, half male and female. All rats were fed a standard basal diet. Rats in Hes group were orally gavaged 300 mg/kg BW hesperidin daily, rats in RA group were orally gavaged 20 mg/kg BW rosmarinic acid daily, rats in Hes×RA group were orally gavaged 150 mg/kg BW hesperidin+10 mg/kg BW rosmarinic acid daily, and rats in LPS group were given equal volume of sterile saline. Gavage treatment was carried out for 14 days during the trial period. On the 13th day, all rats were injected intraperitoneally with 500 μg/kg BW LPS solution to construct acute intestinal injury model. The results showed that the ileal villus height and villus length/crypt depth were significantly increased in Hes×RA group compared with LPS group (P<0.05). High-throughput sequencing results showed that the richness and diversity of ileal digesta microbiota were no significant changes in Hes×RA group compared with LPS group (P<0.05). Compared with LPS group, the relative abundance of Lactobacillus in ileal digesta was significantly increased (P<0.05), while the relative abundances of Actionbacteria, Proteobacteria, Corynebacterium and Streptococcus were significantly reduced in Hes×RA group (P<0.05). Compared with LPS group, the contents of acetate, propionate and total short chain fatty acids in ileal digesta were significantly increased in Hes×RA group (P<0.05). Compared with LPS group, the myeloperoxidase (MPO) activity and the interleukin 6 (IL-6) content in ileal mucosa were significantly reduced (P<0.05), while the interleukin 10 (IL-10) content was significantly increased in Hes×RA group (P<0.05). Compared with LPS group, the mRNA relative expression levels of Claudin-1 and Occludin were significantly increased in Hes×RA group (P<0.05); in addition, the mRNA relative expression level of zonula occludens-1 (ZO-1) trended to increase in Hes×RA group (P=0.064). Compared with LPS group, the mRNA relative expression levels of interleukin-1 receptor associator kinase 1 (IRAK1) and tumor necrosis factor receptor associated protein 6 (TRAF6) were significantly reduced in Hes×RA group (P<0.05). In conclusion, the combination of hesperidin and rosmarinic acid can alleviate the ileal morphology damage of rats which is caused by LPS, and improve the intestinal health by modulating microbiota structure and reducing inflammation response.

Cite this article

LI Panpan , LI Runlin , WU Jiaqing , LEI Mingkang , WANG Jing , ZHU Weiyun . Effects of Hesperidin and Rosmarinic Acid Combination on Ileal Morphology, Microbiota Structure and Inflammatory Response of Lipopolysaccharide-Challenged Rats[J]. Chinese Journal of Animal Nutrition, 2022 , 34(4) : 2689 -2701 . DOI: 10.3969/j.issn.1006-267x.2022.04.062

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