Effects of Mulberry Leaf Extracts with Different Extraction Methods on Growth Performance, Antioxidant and Immune Function in Mice

  • HUANG Hui ,
  • XIA Xin ,
  • ZHOU Yingjun ,
  • LIU Yingying ,
  • WANG Xianze ,
  • WANG Xia ,
  • XIAO Dingfu
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  • 1. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
    2. Geneham Pharmaceutical Co., Ltd., Changsha 410014, China;
    3. Hunan Institute of Animal and Veterinary Science, Changsha 410131, China

Received date: 2021-09-18

  Online published: 2022-04-14

Abstract

The aim of this experiment was to screen out a more effective mulberry leaf extraction method by exploring the effects of different mulberry leaf extracts and mulberry leaf crude extracts on growth performance, antioxidant function, serum biochemical indexes and inflammatory cytokines in mice. In this experiment, the mulberry leaves were percolated extracted with n-hexane, ethyl acetate, ethanol (85%) and water (60 to 70℃), and then the mulberry leaves crude extracts were extracted with water decocting method, which were extracts A, B, C,D and E. A total of 108 five-week-old specific pathogen-free (SPF) grade ICR male mice were randomly divided into 6 groups with 6 repeats and 3 mice for each repeat. The control group was fed a basal diet, and the test groups were respectively fed the basal diet supplemented with mulberry leaf extracts A, B, C, D and E (1 200 mg/kg). The pre-feeding period was 3 days, and the official trial period was 28 days. The results are as follows:compared with the control group, 1)the average daily feed intake (ADFI) of mice in group E was significantly increased (P<0.01); 2) the activity of glutathione peroxidase (GSH-Px) in serum of mice was significantly increased in groups C, D and E (P<0.05). Total superoxide dismutase (T-SOD) activity in serum was significantly increased in groups B, D and E (P<0.01), and serum high-density lipoprotein cholesterol (HDL-C) content was significantly reduced (P<0.05). Aspartate aminotransferase (AST) activity was significantly increased in mice in groups B and D (P<0.05), and serum triglycerides (TG) content in groups B and E was significantly decreased (P<0.05). Albumin (ALB) and total protein (TP) contents in serum were significantly increased in groups A, C D and E (P<0.05). Creatinine (CREA) content in serum was significantly reduced in group C (P<0.05); 3) the content of interleukin (IL)-17 in the liver of mice in groups A, C, D and E were extremely significantly increased (P<0.01), and the content of IL-2 was significantly decreased (P<0.01). The contents of IL-1β and interferon-γ (IFN-γ) in liver in groups A, C, and D were extremely significantly increased (P<0.01), and tumor necrosis factor-α (TNF-α) content in liver in group A was significantly increased (P<0.05). The results show that adding 1 200 mg/kg of mulberry leaf crude extracts to the diet can increase the average daily feed intake of mice, enhance the antioxidant capacity of mice, beneficially regulate serum biochemical indexes, improve liver function, and activate immune response. The crude mulberry leaf extract is screened out to have the best effect.

Cite this article

HUANG Hui , XIA Xin , ZHOU Yingjun , LIU Yingying , WANG Xianze , WANG Xia , XIAO Dingfu . Effects of Mulberry Leaf Extracts with Different Extraction Methods on Growth Performance, Antioxidant and Immune Function in Mice[J]. Chinese Journal of Animal Nutrition, 2022 , 34(4) : 2702 -2711 . DOI: 10.3969/j.issn.1006-267x.2022.04.063

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