Effects of Formonetin on Mucosa Immunity Function in Small Intestinal of Immunosuppressive Mice

MAO Tiantian; BAI Heng; JIA Ning

doi:10.3969/j.issn.1006-267x.2022.04.064

Chinese Journal of Animal Nutrition >

2022 , Vol. 34 >Issue 4: 2712 - 2720

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.04.064

Effects of Formonetin on Mucosa Immunity Function in Small Intestinal of Immunosuppressive Mice

MAO Tiantian ,
BAI Heng ,
JIA Ning

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College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China

Received date: 2021-08-31

Online published: 2022-04-14

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Abstract

In order to reveal the effects of formonetin (FMN) on small intestinal mucosal immune function of immunosuppressed mice. A total of 50 Kunming mice were randomly divided into five groups:normal control group, immunosuppressive model group, and FMN low dose group, medium dose group and high dose group, with 10 mice in each group, and using intragastric administration of medicine to mice. The experiment lasted for 28 days. In the first 7 days, the mice in normal group were given 0.6 mL physiological saline, and the mice in other groups were given 40 mg/(kg·d) cyclophosphamide (CTX) 0.6 mL. In the next 21 days, the mice in normal group and immunosuppressive model group were given 0.6 mL physiological saline, and the mice in the low dose group, medium dose group and high dose group were given 50, 150, and 250 mg/(kg·d) FMN 0.6 mL, respectively. The 24 h after final administration, the mice were killed, the spleen and thymus indexes were detected. The contents of interleukin-2 (IL-2), interleukin-6 (IL-6) and secretory immunoglobulin A (sIgA) in small intestinal mucosal were detected by enzyme linked immunosorbent assay (ELISA). The morphological structure and intraepithelial lymphocytes (IELs), goblet cells of small intestinal mucosa were investigated by conventional paraffin sectioning technique and histochemicaltechique[hematoxylin-eosin (HE) staining and periodate schiff (PAS) staining]. At the same time, the ultrastructural characteristics of small intestinal mucosa epithelium were analyzed by electron microscope technique. The results showed that the immunosuppressed mice were copied successfully with CTX, and the damage of small intestinal mucosal barrier were obviously. All the dose groups of FMN could improve the intestinal mucosal immune function of immunosuppressed mice, among which the effects of the middle dose group of FMN was the most obvious. Compared with the immunosuppressive group, the thymus index, small intestinal mucosal IL-2 and IL-6 contents were significantly different (P<0.05), while the spleen index and intestinal mucosal sIgA contents were extremely different (P<0.01). Meanwhile, FMN could repair the damage of the small intestinal mucosal and increase the villous height and the ratio of villous height to crypt depth. In the electron microscope, FMN could repair the ultrastructural damage and maintain normal structure of the epithelial cells of small intestinal mucosa in immunosuppressed mice. The results suggest that, FMN can significantly improve and promote the small intestinal mucosal immune function in immunosuppressed mice, at the same time, it can also reduce the structural damage of the small intestinal mucosal and enhance their function.

Key words： formonetin; mice; immunosuppression; small intestinal mucosa; mucosal immunity

Cite this article

MAO Tiantian , BAI Heng , JIA Ning . Effects of Formonetin on Mucosa Immunity Function in Small Intestinal of Immunosuppressive Mice[J]. Chinese Journal of Animal Nutrition, 2022 , 34(4) : 2712 -2720 . DOI: 10.3969/j.issn.1006-267x.2022.04.064

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