RESEARCH PAPER

Effects of Phage Cocktail Preparation on Growth Performance, Immune Function, Intestinal Morphology and Gut Microbiota of Broilers

  • ZHU Shujiao ,
  • ZHANG Hui ,
  • ZHOU Yan ,
  • DONG Jun ,
  • DE Yanyan ,
  • ZHANG Yongzhu ,
  • ZHONG Zhaoxin ,
  • WANG Ran ,
  • BAO Hongduo
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  • 1. Key Laboratory of Agro-Product Safety Risk Evaluation (Nanjing), Ministry of Agriculture and Rural Affairs, Institute of Agricultural Product Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Food Quality and Safety of Jiangsu Province, State Key Laboratory Breeding Base, Nanjing 210014, China;
    2. Yixing Tianshi Feed Co., Ltd., Yixing 214200, China;
    3. Jiangsu Hengfengqiang Biotechnology Co., Ltd., Nantong 226100, China;
    4. Institute of Agricultural Sciences in Jiangsu Coastal Area, Yancheng 224002, China

Received date: 2021-09-28

  Online published: 2022-05-14

Abstract

The experiment was conducted to explore the effects of phage cocktail preparation on growth performance, immunity function, intestinal morphology, gut microbiota of broilers. A total of 1 400 thirty-seven-day-old Qingjiao Ma broilers with body weight (850±45) g were randomly divided into 2 groups with 7 replicates per group and 1 000 broiler per replicate:the control group (basic diet) and the experimental group (basic diet+109 PFU/mL phage cocktail preparation). The experiment lasted for 27 days. The results showed as follows:1) compared with the control group, the feed to gain ratio of the experimental group decreased by 2.81% (P>0.05). 2) Compared with the control group, at 43 days of age, the thymus and spleen indexes in the experimental group increased by 16.80% (P<0.05) and 7.14% (P>0.05), respectively; at 52 days of age, the bursa of Fabricius index and thymus indexe in the experimental group increased by 8.55% (P>0.05) and 7.37% (P>0.05), respectively; at 61 days of age, the broiler's bursa of Fabricius index increased by 39.66% (P>0.05); at 43, 52 and 61 days of age, the experimental group increased the secretory immunoglobulin G (IgG) content in serum of broilers by 15.85% (P>0.05), 29.54% (P>0.05) and 1.45% (P>0.05), respectively. 3) Compared with the control group, at 43, 52 and 61 days of age, the ratio of villus height to crypt depth in duodenum, jejunum and ileum of broilers in the experimental group significantly increased (P<0.05). 4) Effects of phage cocktail preparation on the composition and relative abundance of broiler intestinal flora, at the phylum level, compared with the control group, the number of Firmicutes in cecal chyme of 43 days of age broilers in the experimental group increased by 28.13% (P>0.05), and the number of Bacteroidetes decreased by 9.97% (P>0.05); the relative abundance of Proteobacteria in cecal chyme of 52 days of age broilers decreased in the experimental group (P>0.05), and the relative abundance of Spirochaetes increased in the experimental group (P>0.05). The relative abundance of Proteobacteria and Firmicutes in cecal chyme of 61 days of age broilers decreased in the experimental group (P>0.05), while the relative abundance of Bacteroidetes increased (P>0.05). 5) At the genus level, compared with the control group, the experimental group could selectively promote the relative abundance of Parabacteroides, Desulfovibrio and Barnesiella (P>0.05) and reduce the relative abundance of RFN20 (P>0.05); at 61 days of age, the relative abundance of Lactobacillus increased (P>0.05) and RFN20 decreased (P>0.05). In conclusion, phage cocktail preparation is conducive to the improvement of growth performance and immunity of broilers, and can improve intestinal development. In addition, it can strength gut microbiota and promote intestinal health of broilers. Therefore, phage cocktail has potential value in broiler industry.

Cite this article

ZHU Shujiao , ZHANG Hui , ZHOU Yan , DONG Jun , DE Yanyan , ZHANG Yongzhu , ZHONG Zhaoxin , WANG Ran , BAO Hongduo . Effects of Phage Cocktail Preparation on Growth Performance, Immune Function, Intestinal Morphology and Gut Microbiota of Broilers[J]. Chinese Journal of Animal Nutrition, 2022 , 34(5) : 2928 -2939 . DOI: 10.3969/j.issn.1006-267x.2022.05.022

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