RESEARCH PAPER

Meta Analysis of Effects of Probiotics on Intestinal Barrier Function of Weaned Piglets

  • GAO Jingchun ,
  • CAO Shuting ,
  • JIANG Zongyong ,
  • WANG Li
Expand
  • 1. State Key Laboratory of Livestock and Poultry Breeding, Key Laboratory of Animal Nutrition and Feed Science in South China Ministry of Agriculture, Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
    2. Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China

Received date: 2021-10-26

  Online published: 2022-05-14

Abstract

In order to explore the effects of probiotics on intestinal barrier function of weaned piglets, this study conducted a Meta-analysis of relevant studies. Randomized controlled trials on the effects of probiotics supplementation on intestinal health of weaned piglets were searched by searching CNKI, Weipu, Science Direct and Web of Science databases. The retrieval period was from database construction to April 2021. The inclusion and exclusion criteria of samples were set for literature screening and data extraction. A total of 720 samples were included. R language was used for correlation analysis of the included studies, and standardized mean difference (SMD) was selected as the effect index. Meta-analysis results showed that the addition of probiotics in weaned piglets could effectively alleviate diarrhea compared with the blank control group[P<0.01, SMD=-15.15, 95% confidence interval (CI):-21.55 to -8.76] and increase the expression level of zonula occludens protein-1 (ZO-1) (P<0.01, SMD=20.83, 95% CI:8.84 to 32.83), Occludin (P<0.01, SMD=2.587, 95% CI:0.67 to 4.50), promote the growth of jejunum villi (P<0.01, SMD=15.22, 95% CI:5.91 to 24.54). The results indicate that probiotics can protect the intestinal barrier function of weaned piglets.

Cite this article

GAO Jingchun , CAO Shuting , JIANG Zongyong , WANG Li . Meta Analysis of Effects of Probiotics on Intestinal Barrier Function of Weaned Piglets[J]. Chinese Journal of Animal Nutrition, 2022 , 34(5) : 3317 -3328 . DOI: 10.3969/j.issn.1006-267x.2022.05.057

References

[1] 赵志贤.早期断奶应激对仔猪肠道屏障损伤作用的研究[D].硕士学位论文.雅安:四川农业大学, 2013. ZHAO Z X.Reseach of early weaning diarrhea damage on intestinal barrier of piglets[D].Master's Thesis.Ya'an:Sichuan Agricultural University, 2013.(in Chinese)
[2] BARBA-VIDAL E, MARTÍN-OR Ú E S M, CASTILLEJOS L.Review:are we using probiotics correctly in post-weaning piglets?[J].Animal, 2018, 12(12):2489-2498.  
[3] GUEVARRA R B, LEE J H, LEE S H, et al.Piglet gut microbial shifts early in life:causes and effects[J].Journal of Animal Science and Biotechnology, 2019, 10:1.
[4] SMITH H W.Antibiotic-resistant bacteria in animals:the dangers to human health[J].British Veterinary Journal, 1974, 130(2):110-119.  
[5] SPILLER R C.Hidden dangers of antibiotic use:increased gut permeability mediated by increased pancreatic proteases reaching the colon[J].Cellular and Molecular Gastroenterology and Hepatology, 2018, 6(3):347-348.e1.  
[6] 张飞, 李越, 毕丁仁, 等.猪源植物乳杆菌LPZ对断奶仔猪生长性能、免疫指标和粪挥发性脂肪酸的影响[J].中国畜牧杂志, 2020, 56(11):104-109. ZHANG F, LI Y, BI D R, et al.Effects of wine-derived Lactobacillus plantarum LPZ on growth performance, immune index and fecal VFA in weaning piglets[J].Chinese Journal of Animal Science, 2020, 56(11):104-109.(in Chinese)
[7] 王斐, 徐树杰, 李响, 等.益生菌在猪上的应用效果和作用机理研究进展[J].动物营养学报, 2021, 33(11):6044-6056. WANG F, XU S J, LI X, et al.Research progredd on application effects and mechanism of probiotics in pigs[J].Chinese Journal of Animal Nutrition, 2021, 33(11):6044-6056.(in Chinese)
[8] 张定华, 谭占坤, 王静, 等.益生菌对畜禽健康的影响及作用机制研究进展[J].畜牧与饲料科学, 2016, 37(6):117-119, 123. ZHANG D H, TAN Z K, WANG J, et al.Research progress on effect of probiotics on livestock and poultry health and its mechanism[J].Animal Husbandry and Feed Science, 2016, 37(6):117-119, 123.(in Chinese)
[9] SUO C, YIN Y S, WANG X N, et al.Effects of Lactobacillus plantarum ZJ316 on pig growth and pork quality[J].BMC Veterinary Research, 2012, 8:89.
[10] KNECHT D, CHOLEWINSKA P, JANKOWSKA-MAKOSA A, et al.Development of swine, s digestive tract microbiota and its relation to production indices-a review[J].Animals, 2020, 10(3), 527.
[11] 胡宁敏, 陈代文, 余冰, 等.丁酸对断奶仔猪腹泻的缓解作用及其机理研究进展[J].动物营养学报, 2020, 32(5):1973-1979. HU N M, CHEN D W, YU B, et al.Research progress on relaxation effects of butyric acid on diarrhea in weaned piglets and its mechanism[J].Chinese Journal of Animal Nutrition, 2020, 32(5):1973-1979.(in Chinese)
[12] MODINA S C, POLITO U, ROSSI R, et al.Nutritional regulation of gut barrier integrity in weaning piglets[J].Animals, 2019, 9(12):1045.
[13] 周天津, 周雪晴, 万素馨, 等.Meta分析方法及其在医疗卫生领域中的应用[J].重庆医学, 2016, 45(7):985-988. ZHOU T J, ZHOU X Q, WAN S X, et al.Meta analysis method and its application in medical and health field[J].Chongqing Medicine, 2016, 45(7):985-988.(in Chinese)
[14] LU J J, YAO J Y, XU Q Q, et al.Clostridium butyricum relieves diarrhea by enhancing digestive function, maintaining intestinal barrier integrity, and relieving intestinal inflammation in weaned piglets[J].Livestock Science, 2020, 239:104112.
[15] DENG H, XU L, TANG Z R, et al.Effects of orally administered Escherichia coli Nissle 1917 on growth performance and jejunal mucosal membrane integrity, morphology, immune parameters and antioxidant capacity in early weaned piglets[J].Animal Feed Science and Technology, 2014, 198:286-294.
[16] LI Y H, HOU S L, CHEN J S, et al.Oral administration of Lactobacillus delbrueckii during the suckling period improves intestinal integrity after weaning in piglets[J].Journal of Functional Foods, 2019, 63:103591.
[17] 钟晓霞, 黄健, 刘志云, 等.甘露寡糖和复合益生菌对断奶仔猪生长性能及肠道形态结构、挥发性脂肪酸含量和菌群结构的影响[J].动物营养学报, 2020, 32(7):3099-3108. ZHONG X X, HUANG J, LIU Z Y, et al.Effects of mannan oligosaccharides and complex probiotics on growth performance and intestinal morphology, volatile fatty acid contents and microbial structure of weaned piglets[J].Chinese Journal of Animal Nutrition, 2020, 32(7):3099-3108.(in Chinese)
[18] 杨广达.罗伊氏乳杆菌LR1对断奶仔猪生长性能、肠道屏障功能和氨基酸转运的影响[D].硕士学位论文.广州:华南农业大学, 2018. YANG G D.Effects of Lactobacillus reuteri LR1 on the growth performance, intestinal barrier function and amino acid transportation in weaned piglets[D].Master's Thesis.Guangzhou:South China Agricultural University, 2018.(in Chinese)
[19] 庞敏.益生菌制剂对断奶仔猪肠道黏膜屏障功能的影响及其机理的研究[D].硕士学位论文.北京:中国农业科学院, 2016. PANG M.Effects and its mechanism of probiotics on intestinal mucosal barrier function in weaning piglets[D].Master's Thesis.Beijing:Chinese Academy of Agricultural Sciences, 2016.(in Chinese)
[20] 张振斌, 林映才, 蒋宗勇, 等.益生茵对断奶仔猪生长表现、微生物区系和小肠黏膜结构的影响[J].养猪, 2004(1):1-3. ZHANG Z B, LIN Y C, JIANG Z Y, et al.Effects of different probiotics on the growth performance, gut microbial population and the morphology of mucous membrane of small intestine in weaning piglets[J].Swine Production, 2004(1):1-3.(in Chinese)
[21] LI Y X, XIA S T, JIANG X H, et al.Gut microbiota and diarrhea:an updated review[J].Frontiers in Cellular and Infection Microbiology, 2021, 11:625210.
[22] WANG Y Y, YAN X, ZHANG W W, et al.Lactobacillus casei Zhang prevents jejunal epithelial damage to early-weaned piglets induced by Escherichia coli K88 via regulation of intestinal mucosal integrity, tight junction proteins and immune factor expression[J].Journal of Microbiology and Biotechnology, 2019, 29(6):863-876.  
[23] WANG W L, ZIJLSTRA R T, GÄNZLE M G.Feeding Limosilactobacillus fermentum K9-2 and Lacticaseibacillus casei K9-1, or Limosilactobacillus reuteri TMW1.656 reduces pathogen load in weanling pigs[J].Frontiers in Microbiology, 2020, 11:608293.
[24] LUISE D, BERTOCCHI M, MOTTA V, et al.Bacillus sp. probiotic supplementation diminish the Escherichia coli F4ac infection in susceptible weaned pigs by influencing the intestinal immune response, intestinal microbiota and blood metabolomics[J].Journal of Animal Science and Biotechnology, 2019, 10:74.
[25] WIJTTEN P J A, VAN DER MEULEN J, VERSTEGEN M W A.Intestinal barrier function and absorption in pigs after weaning:a review[J].British Journal of Nutrition, 2011, 105(7):967-981.  
[26] 宗秋芳, 钦伟云, 霍永久, 等.猪肠上皮紧密连接蛋白研究进展[J].浙江农业学报, 2018, 30(8):1435-1444. ZONG Q F, QIN W Y, HUO Y J, et al.Research progress of intestinal epithelial tight junction proteins in piglets[J].Acta Agriculturae Zhejiangensis, 2018, 30(8):1435-1444.(in Chinese)
[27] 邹云飞.干酪乳杆菌对猪轮状病毒感染肠组织和肠细胞紧密连接蛋白的影响[D].硕士学位论文.哈尔滨:东北农业大学, 2020. ZOU Y F.Effect of Lactobacillus casei on intestinal tissue and intestinal cell tight junction protein with rotavirus infection[D] Master's Thesis.Harbin:Northeast Agricultural University, 2020.(in Chinese)
[28] 王茹.益生菌E. faecium NCIMB 10415对新生及断奶仔猪生长性能、肠道菌群及免疫功能的影响[D].硕士学位论文.雅安:四川农业大学, 2018. WANG R.Effects of E. faecium NCIMB 10415 supplementation on growth performance, intestinal microbiota and immune function of neonatal and weaned pigs[D].Master's Thesis.Ya'an:Sichuan Agricultural University, 2018.(in Chinese)
[29] 赵彦光, 张斌, 相德才, 等.饲粮中添加复合益生菌对猪生长、粪便菌群及代谢产物的影响[J].中国农业大学学报, 2021, 26(9):124-136. ZHAO Y G, ZHANG B, XIANG D C, et al.Effects of dietary complex probiotics on growth performance, fecal microbiota and metabolite of pigs[J].Journal of China Agricultural University, 2021, 26(9):124-136.(in Chinese)
[30] 李世芳, 赵付荣, 常惠芸, 等.动物肠道菌群的研究进展[J].中国微生态学杂志, 2017, 29(11):1354-1361. LI S F, ZHAO F R, CHANG H Y, et al.Recent progress in the research of animal's intestinal microflora[J].Chinese Journal of Microecology, 2017, 29(11):1354-1361.(in Chinese)
[31] 王德华, 赵志军, 张学英, 等.中国哺乳动物生理生态学研究进展与展望[J].兽类学报, 2021, 41(5):537-555. WANG D H, ZHAO Z J, ZHANG X Y, et al.Research advances and perspectives in mammal physiological ecology in China[J].Acta Theriologica Sinica, 2021, 41(5):537-555.(in Chinese)
Outlines

/