RESEARCH PAPER

Isolation, Identification of Lactobacillus crispatus Y68 from Chickens and Evaluation of Its in Vitro Probiotics Properties

  • ZHANG Jiabao ,
  • MA Ke ,
  • LIU Zhenzhen ,
  • GAO Yu ,
  • LIN Xiaoqi ,
  • YANG Yongjun
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  • College of Veterinary Medicine, Jilin University, Changchun 130062, China

Received date: 2021-09-18

  Online published: 2022-05-14

Abstract

The purpose of this study was to screen and develop lactic acid bacteria for the preparation of probiotics for livestock and poultry, and explore the probiotic properties of L.crispatus Y68. Its probiotic properties were evaluated by antibacterial activity screening, growth curve measurement, acid production, drug sensitivity test, in vitro simulated gastrointestinal condition tolerance test, self-agglutination test and hydrophobicity test. The results showed that L.crispatus Y68 had strong inhibitory effect on Salmonella pullorum ATCC19945 and Staphylococcus aureus USA300, with diameter of bacteriostatic zone of (21.64±0.31) mm and (15.13±0.23) mm, respectively; the logarithmic growth period of L.crispatus Y68 was 6 to 24 h; after 48 h of culture, the pH of the cell suspension was stable at 3.89; L.crispatus Y68 was sensitive to erythromycin, penicillin, cephalosporin, chloramphenicol, vancomycin and clindamycin, but resistant to norfloxacin, ofloxacin, tetracycline, gentamicin and furazolidone; the survival rate of L.crispatus Y68 was 52.61% at pH 2.0 for 3 h, 65.92% at artificial gastric juice for 3 h, 86.23% at artificial intestinal juice for 10 h, and which could tolerate 0.3% bile salt;the self-aggregation rate of L.crispatus Y68 at 2 and 24 h were respectively(29.71±0.40)% and (97.23±0.76)%, and the hydrophobic percentage was (89.59±0.63)%. In conclusion, L.crispatus Y68 is tolerant toward simulated gastrointestinal tract conditions, including low pH, pepsin, pancreatin and bile salts, maintains strong self-aggregation ability and hydrophobic performance. Thus, L.crispatus Y68 from chickens is a potential candidate strain of livestock and poultry probiotics,applied to nutrition and health of livestock and poultry.

Cite this article

ZHANG Jiabao , MA Ke , LIU Zhenzhen , GAO Yu , LIN Xiaoqi , YANG Yongjun . Isolation, Identification of Lactobacillus crispatus Y68 from Chickens and Evaluation of Its in Vitro Probiotics Properties[J]. Chinese Journal of Animal Nutrition, 2022 , 34(5) : 3339 -3347 . DOI: 10.3969/j.issn.1006-267x.2022.05.059

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