RELATED RESEARCH

Effects of Broiler Fertilized Egg Soaked in Ferulic Acid Solution on Chorioallantoie Membrane Angiogenesis of Chicken Embryo and Antioxidant Performance of One-Day-Old Broiler Chickens

  • LAI Shixiong ,
  • ZHENG Yucai ,
  • LI Zhixiong ,
  • TANG Runzi ,
  • YANG Jia ,
  • MA Weimei ,
  • RAO Kaiqing
Expand
  • College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu 610041, China

Received date: 2021-10-08

  Online published: 2022-05-14

Abstract

The purpose of this study was to explore the effects of broiler fertilized egg soaked in ferulic acid solution on chorioallantoie membrane (CAM) angiogenesis of chicken embryo and antioxidant performance of one-day-old broiler chickens, to provide theoretical and experimental basis for the application of ferulic acid in the poultry industry. One hundred and thirty-two yellow chicken fertilized eggs with similar weight[(60±3) g] were divided into 3 groups with 44 eggs per group. When the fertilized eggs were hatched to the 7, 8 and 9 days of embryo, the fertilized eggs in low concentration group and high concentration group were soaked in 40 and 120 mg/L ferulic acid solution for 6 min every day, the fertilized eggs in the control group were soaked in pure water for the same time, and then continued to hatch in the incubator. The results showed as follows:1) compared with the control group, the hatching rate of fertilized eggs of high concentration group decreased by 22%. 2) Compared with the control group, the vascular relative area and number of neovascularization in chicken embryo CAM of low concentration group were significantly increased (P<0.05), while the vascular relative area and number of neovascularization in chicken embryo CAM of high concentration group were significantly decreased (P<0.05). 3) Compared with the control group, the gene expression of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor 2 (FGF2) in chicken embryo CAM of low concentration group were significantly up-regulated (P<0.05). 4) Compared with the control group, the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in serum of high concentration group were significantly increased (P<0.05), and the serum malondialdehyde (MDA) content was significantly decreased (P<0.05); the serum GSH-Px activity of low concentration group was significantly increased (P<0.05); the liver SOD activity and MDA content of low concentration group and high concentration group were significantly decreased (P<0.05). 5) Compared with the control group, the gene expression of SOD in liver of low concentration group and high concentration group was significantly down-regulated (P<0.05). In conclusion, the fertilized eggs soaked in low concentration ferulic acid solution can promote chicken embryo CAM angiogenesis by up-regulate the gene expression levels of VEGF and FGF2 in chicken embryo CAM, while the fertilized eggs soaked in high concentration ferulic acid solution can inhibit chicken embryo CAM angiogenesis and reduce the hatching rate; the fertilized eggs soaked in low and high concentration ferulic acid solution can improve the antioxidant performance of one-day-old broiler chickens.

Cite this article

LAI Shixiong , ZHENG Yucai , LI Zhixiong , TANG Runzi , YANG Jia , MA Weimei , RAO Kaiqing . Effects of Broiler Fertilized Egg Soaked in Ferulic Acid Solution on Chorioallantoie Membrane Angiogenesis of Chicken Embryo and Antioxidant Performance of One-Day-Old Broiler Chickens[J]. Chinese Journal of Animal Nutrition, 2022 , 34(5) : 3392 -3400 . DOI: 10.3969/j.issn.1006-267x.2022.05.063

References

[1] 姚宏, 郑玉才, 李志雄, 等.低氧环境下蛋内注射CuSO4对藏鸡和肉鸡鸡胚发育的影响[J].南京农业大学学报, 2020, 43(6):1079-1086. YAO H, ZHENG Y C, LI Z X, et al.Effects of in ovo injection of CuSO4 on the development of Tibetan chicken and broiler chicken embryos hatched under hypoxia conditions[J].Journal of Nanjing Agricultural University, 2020, 43(6):1079-1086.(in Chinese)
[2] PIESTUN Y, ZIMMERMAN I, YAHAV S.Thermal manipulations of turkey embryos:the effect on thermoregulation and development during embryogenesis[J].Poultry Science, 2015, 94(2):273-280.  
[3] PIESTUN Y, DRUYAN S, BRAKE J, et al.Thermal treatments prior to and during the beginning of incubation affect phenotypic characteristics of broiler chickens posthatching[J].Poultry Science, 2013, 92(4):882-889.  
[4] RIBATTI D.Chick embryo chorioallantoic membrane as a useful tool to study angiogenesis[J].International Review of Cell and Molecular Biology, 2008, 270:181-224.
[5] 顾成波, 宁文娟, 马慧, 等.牡荆苷对鸡胚绒毛尿囊膜血管生成影响研究[J].黑龙江医药, 2018, 31(4):722-725. GU C B, NING W J, MA H, et al.Promoting effect of vitexin on angiogenesis in chick embryo chorioallantoic membrane[J].Heilongjiang Medicine Journal, 2018, 31(4):722-725.(in Chinese)
[6] 苏兰利, 王思敏, 姜雨桦, 等.黄芪多糖浸泡种蛋对雏鸡免疫器官抗氧化性能的影响[J].黑龙江畜牧兽医, 2013, 42(12):133-135. SU L L, WANG S M, JIANG Y H, et al.Effects of Astragalus polysaccharides on antioxidant activity of chicken by soaking fertilized eggs[J].Heilongjiang Animal Science and Veterinary Medicine, 2013, 42(12):133-135.(in Chinese)
[7] 王建平, 徐廷生, 贠银现, 等.种蛋浸泡复合维生素B对鸡胚发育及孵化率的影响[J].畜牧与兽医, 1999, 31(1):19-20. WANG J P, XU T S, YUN Y X, et al.Effects of fertilized egg soaking with complex vitamin B on chicken embryo development and hatching rate[J].Animal Husbandry & Veterinary Medicine, 1999, 31(1):19-20.(in Chinese)
[8] 欧仕益.阿魏酸的功能和应用[J].广州食品工业科技, 2002, 18(4):50-53. OU S Y.The function and application of ferulic acid[J].Guangzhou Food Science & Technology, 2002, 18(4):50-53.(in Chinese)
[9] 刘洋, 戴求仲, 黄兴国, 等.阿魏酸的生物学功能及其在畜禽生产上的应用[J].动物营养学报, 2020, 32(12):5524-5531. LIU Y, DAI Q Z, HUANG X G, et al.Biological functions of ferulic acid and its application in livestock production[J].Chinese Journal of Animal Nutrition, 2020, 32(12):5524-5531.(in Chinese)
[10] 李艳娇, 李黎云, 李蛟龙, 等.日粮添加阿魏酸和维生素E对育肥猪肉品质及抗氧化性能的影响[C]//第七届中国饲料营养学术研讨会论文集, 郑州:中国农业大学出版社, 2014:320. LI Y J, LI L Y, LI J L, et al.Effects of dietary ferulic acid and vitamin E on quality and antioxidant performance of fattening pork[C]//Proceedings of the 7th China Feed Nutrition Symposium.Zhengzhou:China Agricultural University Press, 2014:320.(in Chinese)
[11] VALADEZ-GARCÍA K M, AVENDAÑO-REYES L, DÍAZ-MOLINA R, et al.Free ferulic acid supplementation of heat-stressed hair ewe lambs:oxidative status, feedlot performance, carcass traits and meat quality[J].Meat Science, 2021, 173:108395.
[12] LIN C M, CHIU J H, WU I H, et al.Ferulic acid augments angiogenesis via VEGF, PDGF and HIF-1α[J].The Journal of Nutritional Biochemistry, 2010, 21(7):627-633.  
[13] CALABRESE E J, AGATHOKLEOUS E, CALABRESE V.Ferulic acid and hormesis:biomedical and environmental implications[J].Mechanisms of Ageing and Development, 2021, 198:111544.
[14] 孙晓伟.阿魏酸抑制角膜新生血管的作用及机制研究[J].中国实用医药, 2018, 13(30):193-195. SUN X W.Research of inhibitory effect and mechanism of ferulic acid on corneal neovascularization[J].China Practical Medical, 2018, 13(30):193-195.(in Chinese)
[15] MACÍAS-CRUZ U, PERARD S, VICENTE R, et al.Effects of free ferulic acid on productive performance, blood metabolites, and carcass characteristics of feedlot finishing ewe lambs[J].Journal of Animal Science, 2014, 92(12):5762-5768.  
[16] 运晨霞, 吴光, 王坤.Maf家族蛋白的组成及功能[J].医学分子生物学杂志, 2008, 5(1):82-86. YUN C X, WU G, WANG K.Members and fuctions of the Maf family proteins[J].Journal of Medical Molecular Biology, 2008, 5(1):82-86.(in Chinese)
[17] ZHANG X J, CUI Z H, ZHAO Y X, et al.Ferulic acid ameliorates isoproterenol-induced heart failure by decreasing oxidative stress and inhibiting cardiocyte apoptosis via activating Nrf2 signaling pathway in rats[J].Biological and Pharmaceutical Bulletin, 2021, 44(3):396-403.  
[18] 胡流芳, 王迎, 任汝静, 等.Keap1-Nrf2/ARE信号通路的抗氧化应激作用及其调控机制[J].国际药学研究杂志, 2016, 43(1):146-152, 166. HU L F, WANG Y, REN R J, et al.Anti-oxidative stress actions and regulation mechanisms of Keap1-Nrf2/ARE signal pathway[J].Journal of International Pharmaceutical Research, 2016, 43(1):146-152, 166.(in Chinese)
[19] TAKAGI Y, KOBAYASHI M, LI L, et al.MafT, a new member of the small Maf protein family in zebrafish[J].Biochemical and Biophysical Research Communications, 2004, 320(1):62-69.  
Outlines

/