Effects of Mulberry Leaf Extract on Growth Performance, Nutrient Apparent Digestibility, Antioxidant Ability and Immune Function of Weaned Piglets

SONG Min; WANG Chaopu; DENG Dun; LIU Zhichang; CHEN Qiuyu; CUI Yiyan; LU Huijie; LI Zhenming; YU Miao; TI Junling; MA Xianyong

doi:10.3969/j.issn.1006-267x.2022.06.016

2022 , Vol. 34 >Issue 6: 3537 - 3546

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.06.016

RESEARCHPAPER

Effects of Mulberry Leaf Extract on Growth Performance, Nutrient Apparent Digestibility, Antioxidant Ability and Immune Function of Weaned Piglets

SONG Min ,
WANG Chaopu ,
DENG Dun ,
LIU Zhichang ,
CHEN Qiuyu ,
CUI Yiyan ,
LU Huijie ,
LI Zhenming ,
YU Miao ,
TI Junling ,
MA Xianyong

Expand

1. Guangdong Engineering Technology Research Center of Animal Meat Quality and Safety Control and Evaluation, Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, State Key Laboratory of Livestock and Poultry Breeding, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
2. College of Life Science and Engineering, Foshan University of Science and Technology, Foshan 528225, China;
3. Guangdong Guanghui Agriculture and Animal Husbandry Co., Ltd., Shaoguan 512040, China;
4. Maoming Branch, Guangdong Laboratory for Lingnan Modern Agricultural Science and Technology, Maoming 525000, China

Received date: 2021-11-04

Online published: 2022-06-14

Fold

Abstract

This experiment was designed to investigate the effects of dietary mulberry leaf extract (MLE) on growth performance, nutrient apparent digestibility, digestive enzyme activity, antioxidant ability and immune function of weaned piglets. Forty-eight Duroc×Landrace×Yorkshire castrated weaned piglets with average body weight of (8.287±0.141) kg were selected. Piglets were randomly allotted into 2 groups as the control group (fed a basal diet) and the MLE group (fed the basal diet+1 000 g/t MLE) according to body weight, each group consisted of 6 replicates and 4 pigs per replicate. The experiment lasted for 28 days. The results showed as follows:1) there were no significant differences in average daily feed intake (ADFI) and feed/gain (F/G) of weaned piglets between the two groups (P>0.05), but compared with the control group, the average daily gain (ADG) in the MLE group had an increasing trend (0.05 ≤ P<0.10), and the diarrhea rate was significantly lower than that in the control group (P<0.05). 2) Compared with the control group, the apparent digestibility of ether extract in the MLE group was significantly increased (P<0.05), and the apparent digestibility of dry matter tended to be increased (0.05 ≤ P<0.10). 3) Compared with the control group, the lipase activity in jejunum and ileum of weaned piglets in the MLE group was significantly increased (P<0.05). 4) Compared with the control group, dietary MLE significantly increased the glutathione peroxidase (GSH-Px) activity and total antioxidant capacity (T-AOC) in plasma as well as the total superoxide dismutase (T-SOD) activity in liver of weaned piglets (P<0.05). 5) Compared with the control group, the plasma tumor necrosis factor-α (TNF-α) content of weaned piglets in the MLE group was significantly reduced (P<0.05), and the contents of immunoglobulin A (IgA) and immunoglobulin M (IgM) in plasma were significantly increased (P<0.05). In conclusion, dietary 1 000 g/t MLE can reduce the diarrhea rate, improve intestinal lipase activity and apparent digestibility of ether extract, and improve antioxidant ability and immune function of weaned piglets.

Key words： mulberry leaf extract; weaned piglets; nutrient apparent digestibility; digestive enzyme activity; antioxidant ability; immune function

Cite this article

SONG Min , WANG Chaopu , DENG Dun , LIU Zhichang , CHEN Qiuyu , CUI Yiyan , LU Huijie , LI Zhenming , YU Miao , TI Junling , MA Xianyong . Effects of Mulberry Leaf Extract on Growth Performance, Nutrient Apparent Digestibility, Antioxidant Ability and Immune Function of Weaned Piglets[J]. Chinese Journal of Animal Nutrition, 2022 , 34(6) : 3537 -3546 . DOI: 10.3969/j.issn.1006-267x.2022.06.016

References

[1] GRESSE R, CHAUCHEYRAS-DURAND F, FLEURY M A, et al.Gut microbiota dysbiosis in postweaning piglets:understanding the keys to health[J].Trends in Microbiology, 2017, 25(10):851-873.

[2] HEO J M, OPAPEJU F O, PLUSKE J R, et al.Gastrointestinal health and function in weaned pigs:a review of feeding strategies to control post-weaning diarrhoea without using in-feed antimicrobial compounds[J].Journal of Animal Physiology and Animal Nutrition, 2013, 97(2):207-237.

[3] 高雨飞, 黎力之, 欧阳克蕙, 等.桑叶在肉牛生产中的开发利用[J].中国牛业科学, 2015, 41(1):68-70, 75. GAO Y F, LI L Z, OUYANG K H, et al.The development and utilization of mulberry leaf in beef cattle production[J].China Cattle Science, 2015, 41(1):68-70, 75.(in Chinese)
[4] ANDALLU B, SURYAKANTHAM V, LAKSHMI SRIKANTHI B, et al.Effect of mulberry (Morus indica L.) therapy on plasma and erythrocyte membrane lipids in patients with type 2 diabetes[J].Clinica Chimica Acta, 2001, 314(1/2):47-53.
[5] HOLLMAN P C, KATAN M B.Dietary flavonoids:intake, health effects and bioavailability[J].Food and Chemical Toxicology, 1999, 37(9/10):937-942.
[6] RAMOS S.Cancer chemoprevention and chemotherapy:dietary polyphenols and signalling pathways[J].Molecular Nutrition & Food Research, 2010, 52(5):507-526.
[7] 朱琳, 赵金鸽, 范作卿, 等.桑叶的主要营养成分及其药理作用的研究进展[J].北方蚕业, 2017, 38(2):9-15, 23. ZHU L, ZHAO J G, FAN Q, et al.Research progress on nutrient components, biological activity and application of mulberry leaves[J].North Sericulture, 2017, 38(2):9-15, 23.(in Chinese)
[8] 黄添就, 闭海龙, 陈芳艳, 等.不同品种桑叶单宁和凝集素含量的检测及评价[J].广东蚕业, 2021, 55(5):7-9. HUANG T J, BI H L, CHEN F Y, et al.The determination and evaluation of tannin and lectin in different varieties mulberry leaves[J].Guangdong Sericulture, 2021, 55(5):7-9.(in Chinese)
[9] 雷春龙, 李娟, 吴永胜, 等.桑枝叶提取物对蛋鸡生产性能、血清免疫指标与抗氧化能力的影响[J].中国畜牧杂志, 2019, 55(8):118-122. LEI C L, LI J, WU Y S, et al.Effects of mulberry branch and leaf extract on growth performance, serum immune indexes and antioxidant capacity of laying hens[J].Chinese Journal of Animal Science, 2019, 55(8):118-122.(in Chinese)
[10] 范京辉, 张永华, 楼立峰, 等.桑叶及其提取物对AA肉鸡生长、养分消化与胴体品质的影响[J].杭州农业与科技, 2012(5):29-32. FAN J H, ZHANG Y H, LOU L F, et al.Effects of mulberry leaf and its extracts on growth, nutrient digestion and carcass quality of AA broilers[J].Hangzhou Agricultural Science and Technology, 2012(5):29-32.(in Chinese)
[11] 赵卫国, 孙梦琦, 侯启瑞, 等.日粮中添加桑叶粉对扬州鹅饲料利用率及生长和屠宰性能的影响[J].蚕业科学, 2019, 45(3):386-392. ZHAO W G, SUN M Q, HOU Q R, et al.Effect of adding mulberry leaf powder to daily diet on feed utilization rate, growth and slaughter performance of Yangzhou goose[J].Acta Sericologica Sinica, 2019, 45(3):386-392.(in Chinese)
[12] SHEIKHLAR A, ALIMON A R, DAUD H, et al.White mulberry (Morus alba) foliage methanolic extract can alleviate Aeromonas hydrophila infection in African catfish (Clarias gariepinus)[J].The Scientific World Journal, 2014, 2014:592709.
[13] 卢桦, 李战福, 黄先智, 等.桑叶提取物和1-脱氧野尻霉素对大鲵生长性能、肝脏功能及免疫能力的影响[J].动物营养学报, 2021, 33(1):584-593. LU H, LI Z F, HUANG X Z, et al.Effects of mulberry leaf extract and 1-deoxynojirimycin on growth performance, liver function and immune ability of giant salamander (Andrias davidianus)[J].Chinese Journal of Animal Nutrition, 2021, 33(1):584-593.(in Chinese)
[14] HAS H, YUNIANTO V D, SUKAMTO B.The effectivity of fermented mulberry leaves with rumen liquor as broiler feed on final body weight, dry matter and crude fiber digestibility, and metabolic energy[J].Animal Production, 2013, 15(3):173-179.
[15] ZHAO X J, LI L, LUO Q L, et al.Effects of mulberry (Morus alba L.) leaf polysaccharides on growth performance, diarrhea, blood parameters, and gut microbiota of early-weanling pigs[J].Livestock Science, 2015, 177:88-94.
[16] 李婉涛, 王义翠, 徐秋良, 等.杜仲和桑叶提取物对育肥猪生长性能及猪肉品质的影响[J].黑龙江畜牧兽医, 2018(10):157-160. LI W T, WANG Y C, XU Q L, et al.Effect of Eucommia ulmoicles Oliv. and mulberry leaf extract on growth performance and pork quality of fattening pigs[J].Heilongjiang Animal Science and Veterinary Medicine, 2018(10):157-160.(in Chinese)
[17] JAYARAMAN B, NYACHOTI C M.Husbandry practices and gut health outcomes in weaned piglets:a review[J].Animal Nutrition, 2017, 3(3):205-211.

[18] WANG B, YANG C T, DIAO Q Y, et al.The influence of mulberry leaf flavonoids and Candida tropicalis on antioxidant function and gastrointestinal development of preweaning calves challenged with Escherichia coli O141:K99[J].Journal of Dairy Science, 2018, 101(7):6098-6108.

[19] CHEN D D, CHEN X L, TU Y, et al.Effects of mulberry leaf flavonoid and resveratrol on methane emission and nutrient digestion in sheep[J].Animal Nutrition, 2015, 1(4):362-367.

[20] LI Z F, CHEN X C, CHEN Y J, et al.Effects of dietary mulberry leaf extract on the growth, gastrointestinal, hepatic functions of Chinese giant salamander (Andrias davidianus)[J].Aquaculture Research, 2020, 51(6):2613-2623.

[21] KIM D S, KANG Y M, JIN W Y, et al.Antioxidant activities and polyphenol content of Morus alba leaf extracts collected from varying regions[J].Biomedical Reports, 2014, 2(5):675-680.

[22] RADOJKOVIĆ M M, ZEKOVIĆ Z P, VIDOVIĆ S S, et al.Free radical scavenging activity, total phenolic and flavonoid contents of mulberry (Morus spp. L., Moraceae) extracts[J].Hemijska Industrija, 2012, 66(4):547-552.

[23] NAOWABOOT J, PANNANGPETCH P, KUKONGVIRIYAPAN V, et al.Antihyperglycemic, antioxidant and antiglycation activities of mulberry leaf extract in streptozotocin-induced chronic diabetic rats[J].Plant Foods for Human Nutrition, 2009, 64(2):116-121.

[24] ANDALLU B, VARADACHARYULU N C.Antioxidant role of mulberry (Morus indica L. cv. Anantha) leaves in streptozotocin-diabetic rats[J].Clinica Chimica Acta, 2003, 338(1/2):3-10.
[25] 王霞, 肖定福.桑叶提取物对动物免疫功能的调节作用及其机理研究进展[J].饲料博览, 2020(12):9-13, 19. WANG X, XIAO D F.Research progress on the effect of mulberry leaf extract on immune function and its mechanism[J].Feed Review, 2020(12):9-13, 19.(in Chinese)
[26] 沈维治, 邹宇晓, 刘凡, 等.桑叶不同极性溶剂提取物的总多酚含量与抑菌活性[J].蚕业科学, 2013, 39(1):135-138. SHEN W Z, ZOU Y X, LIU F, et al.Total polyphenol content and antibacterial activity of mulberry leaf extracts from different polarity solvents[J].Science of Sericulture, 2013, 39(1):135-138.(in Chinese)
[27] HORNG C T, LIU Z H, HUANG Y T, et al.Extract from mulberry (Morus australis) leaf decelerate acetaminophen induced hepatic inflammation involving downregulation of myeloid differentiation factor 88 (MyD88) signals[J].Journal of Food and Drug Analysis, 2017, 25(4):862-871.

[28] XUE M, SUN H Y, CAO Y, et al.Mulberry leaf polysaccharides modulate murine bone-marrow-derived dendritic cell maturation[J].Human Vaccines & Immunotherapeutics, 2015, 11(4):946-950.

[29] 侯瑞宏, 廖森泰, 刘凡, 等.桑叶多糖对小鼠免疫调节作用的影响[J].食品科学, 2011, 32(13):280-283. HOU R H, LIAO S T, LIU F, et al.Immunomodulatory effect of polysaccharides from mulberry leaves (PML) in mice[J].Food Science, 2011, 32(13):280-283.(in Chinese)
[30] BERCIER P, GRENIER D.TNF-α disrupts the integrity of the porcine respiratory epithelial barrier[J].Research in Veterinary Science, 2019, 124:13-17.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References