RESEARCHPAPER

Effects of Dietary Crude Protein Level on Liver Lipid Metabolism and Bile Acid Metabolism of Growing Hens

  • YUAN Yihan ,
  • SHEN Yu ,
  • YANG Ye
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  • College of Animal Science, Yangtze University, Jingzhou 434000, China

Received date: 2021-11-16

  Online published: 2022-06-14

Abstract

This experiment was conducted to investigate the effects of dietary crude protein level on liver lipid metabolism and bile acid (BA) metabolism of growing hens. One hundred and eighty hens at 14 weeks of age were randomly divided into 3 groups, with 6 replicates in each group and 10 hens in each replicate. The three groups of hens were fed 15.5% high protein level diet (CP-H group), 14.5% normal protein level diet (CP-M group) and 13.5% low protein level diet (CP-L group), of which the other nutrients were at the same level. The preliminary trial period lasted for 1 week and formal trial period lasted for 3 weeks. The results showed as follows:1) dietary crude protein level had no significant effects on initial weight (IW), final weight (FW), average daily gain (ADG) and average daily feed intake (ADFI)(P>0.05). 2) Compared with the CP-H group, the fat content in breast muscle and leg muscle, the percentage of abdominal fat, and contents of triglyceride (TG) and total cholesterol (TC) in liver were significantly increased (P<0.05). 3) Compared with CP-H group, the contents of TC, high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) in serum in CP-L group were significantly increased (P<0.05), and the contents of serum very low density lipoprotein (VLDL), uric acid (UA) and total protein (TP) in CP-L group were significantly reduced (P<0.05). 4) Compared with CP-H group, the contents of lithocholic acid (LCA) and ursodesoxycholic acid (UDCA) in CP-L group were significantly decreased (P<0.05), and the deoxycholic acid (DCA) content was significantly increased (P<0.05). 5) The results of gene expression related to lipid metabolism and bile acids metabolism in liver showed that the gene related expression levels of fatty acid translocase (FAT/CD6), fatty acid synthase (FAS), CCAAT/enhancer binding protein β (C/EBPβ) in CP-L group were significantly higher than those in CP-H group (P<0.05), while the gene related expression levels of lipoprotein lipase (LPL), apolipoprotein very low density lipoprotein (apoVLDL), derivatives of farnesol X receptor (FXR) and cholesterol 7α-hydroxylase (CYP7A1) in CP-L group were significantly lower than those in CP-H group (P<0.05). In conclusion, 13.5% low crude protein level diet has no effect on growth performance of growing hens, but it can increase the lipid deposition in liver and effects the health of liver by influencing the lipid metabolism and bile acid metabolism in liver.

Cite this article

YUAN Yihan , SHEN Yu , YANG Ye . Effects of Dietary Crude Protein Level on Liver Lipid Metabolism and Bile Acid Metabolism of Growing Hens[J]. Chinese Journal of Animal Nutrition, 2022 , 34(6) : 3583 -3592 . DOI: 10.3969/j.issn.1006-267x.2022.06.020

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