Effects of Cadmium, Lead, Mercury, Chromium and Selenium Co-Treatment on Metal Ion Homeostasis in Liver, Kidney, Serum and Tibia of Laying Hens

ZHANG Yuwei; LI Lang; JIANG Yuxuan; SONG Jingping; LIU Guangmang; WANG Jianping; LIN Yan; BAI Shiping; ZHANG Keying; WU Caimei

doi:10.3969/j.issn.1006-267x.2022.06.021

2022 , Vol. 34 >Issue 6: 3593 - 3603

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.06.021

RESEARCHPAPER

Effects of Cadmium, Lead, Mercury, Chromium and Selenium Co-Treatment on Metal Ion Homeostasis in Liver, Kidney, Serum and Tibia of Laying Hens

ZHANG Yuwei ,
LI Lang ,
JIANG Yuxuan ,
SONG Jingping ,
LIU Guangmang ,
WANG Jianping ,
LIN Yan ,
BAI Shiping ,
ZHANG Keying ,
WU Caimei

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Key Laboratory for Animal Disease-Resistance Nutrition and Feedstuffs of China Ministry of Agriculture and Rural Affairs and Sichuan Province, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, China

Received date: 2021-12-05

Online published: 2022-06-14

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Abstract

The objective of this experiment was to explore the dietary co-treatment of heavy metal[cadmium (Cd), lead (Pb), mercury (Hg) and chromium (Cr)] on metal ion homeostasis toxicity in liver, kidney, serum and tibia of laying hens, as well as the ameliorative effect of selenium (Se). A total of 160 healthy 63-week-old Lohmann pink-shell laying hens were randomly divided into 4 groups with 10 replicates per group and 10 hens per replicate. Hens in the control group (CON group) were fed a corn-soybean meal basal diet, hens in the adding selenium group (Se group) were fed the basal diet supplemented with 0.4 mg/kg Se (selenium yeast), hens in the heavy metal co-treatment group (HEM group) were fed the basal diet supplemented with 5 mg/kg Cd (CdCl₂), 5 mg/kg Cr (CrCl₃), 50 mg/kg Pb[Pb(NO₃)₂] and 3 mg/kg Hg (HgCl₂), and hens in the heavy metal co-treatment adding selenium group (HEM+Se group) were fed the HEM group diet supplemented with 0.4 mg/kg Se (selenium yeast). The pre-experimental period lasted for 1 week, and the experimental period lasted for 12 weeks. At the end of the experiment, two hens were selected from each replicate, the blood was collected from pterygoid vein and then slaughtered, the liver, kidney and tibia samples were collected, and the contents of Cd, Pb, Hg, Cr, Se, copper (Cu), iron (Fe), manganese (Mn), zinc (Zn), sodium (Na), potassium (K), magnesium (Mg) and calcium (Ca) were measured. The results showed as follows:1) compared with the CON group, the contents of Cd, Pb and Hg in liver, kidney and tibia were significantly increased (P<0.05). Compared with the HEM group, the contents of Cd and Pb in liver of HEM+Se group were no significant difference (P>0.05), the contents of Cd, Pb and Hg in kidney were no significant difference (P>0.05), and the contents of Cd and Hg in tibia were no significant difference (P>0.05). 2) Compared with the CON group, the contents of Fe, Zn and K in liver were significantly increased (P<0.05), the contents of Se, Mn, Mg and Ca in serum were significantly decreased (P<0.05), and the contents of Se, Na and K in tibia were significantly increased (P<0.05). Compared with the HEM group, the contents of Fe, Zn and K in liver of HEM+Se group were no significant difference (P>0.05), the contents of Mn, Mg and Ca in serum were no significant difference (P>0.05), and the tibia Mn content was no significant difference (P>0.05). 3) The correlation analysis results showed that there were 19, 12, 7 and 12 significant correlations among the contents of 13 metal ions in liver, kidney, tibia and the serum of laying hens (P<0.05), respectively. In summary, under the condition of this experiment, dietary co-treatment of heavy metal can induce the tissue metal ion disorder, and increase the corresponding heavy metal deposition, and disturb the metal ion homeostasis in liver, kidney, serum and tibia of laying hens. Dietary 0.4 mg/kg Se dose not alleviate the metal ion disorder toxicity cause by heavy metal treatment.

Key words： heavy metal; selenium; metal ion homeostasis; laying hens

Cite this article

ZHANG Yuwei , LI Lang , JIANG Yuxuan , SONG Jingping , LIU Guangmang , WANG Jianping , LIN Yan , BAI Shiping , ZHANG Keying , WU Caimei . Effects of Cadmium, Lead, Mercury, Chromium and Selenium Co-Treatment on Metal Ion Homeostasis in Liver, Kidney, Serum and Tibia of Laying Hens[J]. Chinese Journal of Animal Nutrition, 2022 , 34(6) : 3593 -3603 . DOI: 10.3969/j.issn.1006-267x.2022.06.021

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