RESEARCHPAPER

Effects of Different Phosphorus Sources and Levels on Laying Performance and Bone Metabolism of Laying Hens Aged from 69 to 78 Weeks

  • REN Yuechang ,
  • DENG Ximin ,
  • CHEN Zhengping ,
  • SHAN Zhongfang ,
  • LIU Yaping ,
  • MA Lulu ,
  • ZHANG Pengfei ,
  • WAN Boyang ,
  • YANG Weiren
Expand
  • 1. Shandong Provincial Key Laboratory of Animal Bioengineering and Disease Control, College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271018, China;
    2. Weifang Limin Feed Co., Ltd., Weifang 261000, China;
    3. Agricultural and Rural Center, Shazikou Street, Laoshan District, Qingdao City, Qingdao 266000, China;
    4. Jinan Spafas Poultry Co., Ltd., Ji'nan 250000, China

Received date: 2021-11-10

  Online published: 2022-06-14

Abstract

This experiment was conducted to investigate the effects of different phosphorus sources and levels and phytase supplementation on performance, egg quality and bone metabolism of laying hens aged from 69 to 78 weeks, and to provide theoretical basis for dietary phosphorus source selection and phosphorus supplemental level of laying hens after peak season. A total of 2 475 HyLine brown laying hens of 69-week-old were randomly divided into 11 groups with 5 replicates per group and 45 hens per replicate. Control group was fed a corn-soybean meal basal diet (without any additional phosphorus source), and the experimental groups were treated with calcium hydrogen phosphate (DCP)[non-phytate phosphorus (NPP) supplemental levels were 0.10%, 0.15%, 0.20%, 0.25% and 0.30%, respectively] and mono dicalcium phosphate (MDCP) (NPP supplemental levels were 0.07%, 0.11%, 0.15%, 0.18% and 0.22%, respectively) as exogenous phosphorus sources. All diets were supplemented with 1 470 FTU/kg phytase. The pre-trial period lasted for 1 week, and the trial period lasted for 8 weeks. The results showed as follows:1) different phosphorus sources and levels had no significant effects on average laying rate, average egg weight, feed to egg ratio and abnormal egg rate of laying hens aged from 69 to 78 weeks (P>0.05). However, compared with the control group, MDCP as phosphorus source significantly increased the average daily feed intake (P<0.05). 2) Compared with the control group and DCP group, MDCP as phosphorus source significantly improved yolk color grade (P<0.05), and 0.18% NPP supplemental level group had the highest yolk color grade. Compared with the control group, DCP as phosphorus source had no significant effect on yolk color grade (P>0.05). 3) The contents of parathyroid hormone (PTH), osteoprotegerin (OPG) and Ⅰ type collagen C-terminal peptide (CTX-Ⅰ) as well as tartrate-resistant acid phosphatase (TRACP) activity in serum of laying hens in the control group were the highest, and compared with the control group, the serum PTH content in DCP groups supplemented with 0.15%, 0.25% and 0.30% NPP was significantly decreased (P<0.05), the serum OPG content in DCP group supplemented with 0.15% to 0.30% NPP and MDCP group supplemented with 0.11% to 0.18% NPP was significantly decreased (P<0.05), and the serum TRACP activity in DCP group supplemented with 0.15% to 0.30% NPP and MDCP group supplemented with 0.11% to 0.22% NPP was significantly decreased (P<0.05). Different phosphorus sources and levels had no significant effects on serum contents of calcitonin (CT), 1,25-dihydroxy vitamin D3[1,25-(OH)2-VD3], osteocalcin (BGP) and osteopontin (OPN) as well as bone alkaline phosphatase (BAP) activity of laying hens (P>0.05). In conclusion, under the condition of adding 1 470 FTU/kg phytase, reducing dietary NPP level has no negative effects on laying rate and feed to egg ratio of laying hens aged from 69 to 78 weeks, but MDCP as phosphorus source can improve yolk color grade. Considering performance, inorganic phosphorus consumption and bone metabolic health, it is recommended that MDCP be used as phosphorus source in the diet after peak season, and the appropriate NPP supplemental level is 0.11% (dietary NPP level is 0.23%).

Cite this article

REN Yuechang , DENG Ximin , CHEN Zhengping , SHAN Zhongfang , LIU Yaping , MA Lulu , ZHANG Pengfei , WAN Boyang , YANG Weiren . Effects of Different Phosphorus Sources and Levels on Laying Performance and Bone Metabolism of Laying Hens Aged from 69 to 78 Weeks[J]. Chinese Journal of Animal Nutrition, 2022 , 34(6) : 3604 -3614 . DOI: 10.3969/j.issn.1006-267x.2022.06.022

References

[1] 王秀静.不同无机磷源对断奶仔猪生长性能、肠道形态结构及肠道稳态的影响[D].硕士学位论文.南昌:江西农业大学, 2015. WANG X J.Regulation of different inorganic phosphorus source on growth performance, intestinal morphology structure and intestinal steady state of weaned piglets[D].Master's Thesis.Nanchang:Jiangxi Agricultural University, 2015.(in Chinese)
[2] 杨露晴, 刘国庆, 马秋刚, 等.小麦-DDGS饲粮磷水平对京红代蛋鸡产蛋后期产蛋性能、蛋壳品质的影响[J].饲料研究, 2019, 42(8):29-32. YANG L Q, LIU G Q, MA Q G, et al.Effect of dietary phosphorus level in wheat-DDGS diet on laying performance and shell quality during late period of Jinghong laying hens[J].Feed Research, 2019, 42(8):29-32.(in Chinese)
[3] 谭占坤.磷来源和水平对蛋鸡生产性能、蛋品质和骨骼质量的影响[D].硕士学位论文.雅安:四川农业大学, 2011. TAN Z K.Effect of phosphorus source and level on performance, egg quality, and bone quality of laying hens[D].Master's Thesis.Ya'an:Sichuan Agricultural University, 2011.(in Chinese)
[4] 敖翔, 何健.磷在肉仔鸡上的营养作用及其生物学利用率的研究进展[J].饲料与畜牧, 2018(5):59-64. AO X, HE J.Research progress on nutritional effect and biological utilization of phosphorus in broilers[J].Feed and Husbandry, 2018(5):59-64.(in Chinese)
[5] 王菊, 赵华, 陈小玲, 等.饲粮非植酸磷水平对肉鸭生长性能和消化功能的影响[J].动物营养学报, 2020, 32(1):206-214. WANG J, ZHAO H, CHEN X L, et al.Effects of dietary non-phytate phosphorus level on growth performance and digestive function of meat ducks[J].Chinese Journal of Animal Nutrition, 2020, 32(1):206-214.(in Chinese)
[6] CALVO M S, TUCKER K L.Is phosphorus intake that exceeds dietary requirements a risk factor in bone health?[J].Annals of the New York Academy of Sciences, 2013, 1301(1):29-35.  
[7] SOHAIL S S, ROLAND D A.Influence of dietary phosphorus on performance of Hy-Line W36 hens[J].Poultry Science, 2002, 81(1):75-83.  
[8] 万敏艳, 张保海, 王宏博, 等.磷酸一二钙对肉仔鸡相对生物学利用率的研究[J].动物营养学报, 2018, 30(9):3353-3363. WAN M Y, ZHANG B H, WANG H B, et al.A study of relative bioavailability of mono-dicalcium phosphate on broilers[J].Chinese Journal of Animal Nutrition, 2018, 30(9):3353-3363.(in Chinese)
[9] 万荣, 谢木林.新型磷源Ⅲ型磷酸氢钙在肉鸡上相对生物学利用率的应用研究[J].饲料广角, 2014(16):16-19. WAN R, XIE M L.Application of a new phosphorus source type Ⅲ calcium hydrogen phosphate in broilers[J].Feed China, 2014(16):16-19.(in Chinese)
[10] 刘景, 陈炳钿, 李忠荣, 等.磷酸一二钙不同添加水平对肉鸡后期生产性能、胫骨和血液指标的影响[J].家畜生态学报, 2021, 42(4):40-44. LIU J, CHEN B D, LI Z R, et al.Effects of different levels of monocalcium phosphate on production performance, tibia and blood parameters of broilers at late stage[J].Acta Ecologae Animalis Domastici, 2021, 42(4):40-44.(in Chinese)
[11] 龚家竹.我国饲料磷酸盐技术发展纪事[J].磷肥与复肥, 2018, 33(12):38-43, 69. GONG J Z.Development notes of feed-grade phosphate technology in China[J].Phosphate & Compound Fertilizer, 2018, 33(12):38-43, 69.(in Chinese)
[12] NIE W, YANG Y, YUAN J M, et al.Effect of dietary nonphytate phosphorus on laying performance and small intestinal epithelial phosphate transporter expression in Dwarf pink-shell laying hens[J].Journal of Animal Science and Biotechnology, 2013, 4(1):34.
[13] JING M, ZHAO S, ROGIEWICZ A, et al.Assessment of the minimal available phosphorus needs of laying hens:implications for phosphorus management strategies[J].Poultry Science, 2018, 97(7):2400-2410.  
[14] 耿爱莲, 赵向红, 张尧, 等.饲粮有效磷水平对散养北京油鸡生产性能和鸡蛋品质的影响[J].中国家禽, 2015, 37(18):18-21. GENG A L, ZHAO X H, ZHANG Y, et al.Effects of dietary available phosphorus levels on production performance and egg quality of Beijing You chicken under free range system[J].China Poultry, 2015, 37(18):18-21.(in Chinese)
[15] 程曦.饲粮非植酸磷水平对蛋鸡产蛋性能和磷代谢的影响[D].硕士学位论文.杨凌:西北农林科技大学, 2019. CHENG X.Effects of dietary non-phytate phosphorus levels on laying performance and phosphorus metabolism of laying hens[D].Master's Thesis.Yangling:Northwest A & F University, 2019.(in Chinese)
[16] 李树鹏, 郝艳霜, 赵国先, 等.饲粮钙磷水平对太行鸡产蛋性能和蛋品质的影响[J].北方牧业, 2021(20):19-20, 23. LI S P, HAO Y S, ZHAO G X, et al.Effects of dietary calcium and phosphorus levels on laying performance and egg quality of Taihang chickens[J].Northern Journal of Animal Husbandry, 2021(20):19-20, 23.(in Chinese)
[17] 孙飞.不同磷水平和钙磷比对高产蛋鸡蛋品质的影响[J].湖南饲料, 2016(6):38-41. SUN F.Effects of different phosphorus levels and calcium phosphorus ratio on the quality of high-yield eggs[J].Hunan Feed, 2016(6):38-41.(in Chinese)
[18] 聂伟.日粮磷水平对矮小型蛋鸡产蛋性能、蛋壳品质及钙磷吸收的影响[D].博士学位论文.北京:中国农业大学, 2013. NIE W.Effects of dietary phosphorus levels on laying performance、egg shell quality and Ca and P absorption in laying hens with drawf gene[D].Ph.D.Thesis.Beijing:China Agricultural University, 2013.(in Chinese)
[19] PANDA A K, RAO S V R, RAJU M V L N, et al.Effects of dietary non-phytate phosphorus levels on egg production, shell quality and nutrient retention in white leghorn layers[J].Asian-Australasian Journal of Animal Sciences, 2005, 18(8):1171-1175.  
[20] KESHAVARZ K, NAKAJIMA S.Re-evaluation of calcium and phosphorus requirements of laying hens for optimum performance and eggshell quality[J].Poultry Science, 1993, 72(1):144-153.  
[21] 孙文强.产蛋鸡在不同磷水平饲粮下的磷营养过程分析[D].硕士学位论文.杨凌:西北农林科技大学, 2018. SUN W Q.Analysis of phosphorus nutrition process in laying hens fed diets with different phosphorus levels[D].Master's Thesis.Yangling:Northwest A & F University, 2018.(in Chinese)
[22] 杜光波, 王友刚, 蒋蓓蕾.日粮高水平非植酸磷对肉鹅生长性能、胫骨成分含量及钙磷代谢的影响[J].中国饲料, 2018(16):27-31. DU G B, WANG Y G, JIANG B L.Effects of dietary high non-phytate phosphorus level on growth performance, tibia bone composition and metabolism of calcium and phosphorus of geese[J].China Feed, 2018(16):27-31.(in Chinese)
[23] 章世元, 俞路, 王雅倩, 等.不同磷水平对老龄笼养蛋鸡内分泌及骨密度的影响[J].江苏农业学报, 2009, 25(1):142-146. ZHANG S Y, YU L, WANG Y Q, et al.Effects of phosphorus level on endocrine and bone density of old cage layers[J].Jiangsu Journal of Agricultural Sciences, 2009, 25(1):142-146.(in Chinese)
[24] 张萌萌, 张秀珍, 邓伟民, 等.骨代谢生化指标临床应用专家共识 (2019)[J].中国骨质疏松杂志, 2019, 25(10):1357-1372. ZHANG M M, ZHANG X Z, DENG W M, et al.Expert consensus on clinical application of biochemical indicators of bone metabolism (2019)[J].Chinese Journal of Osteoporosis, 2019, 25(10):1357-1372.(in Chinese)
[25] SAHIN ERSOY G, GIRAY B, SUBAS S, et al.Interpregnancy interval as a risk factor for postmenopausal osteoporosis[J].Maturitas, 2015, 82(2):236-240.  
[26] NIIMI R, KONO T, NISHIHARA A, et al.Determinants associated with bone mineral density increase in response to daily teriparatide treatment in patients with osteoporosis[J].Bone, 2014, 66:26-30.
[27] BOROŃ D, KOTRYCH D, BARTKOWIAK-WIECZOREK J, et al.Polymorphisms of OPG and their relation to the mineral density of bones in pre- and postmenopausal women[J].International Immunopharmacology, 2015, 28(1):477-486.  
[28] FURUYA Y, UCHIDA K, YASUDA H.Stimulation of bone formation in cortical bone of the mice treated with a novel bone anabolic peptide with osteoclastogenesis inhibitory activity[J].Arthritis Research & Therapy, 2012, 14(1):P20.
[29] 郭文文.饲粮不同磷水平和钙磷比添加植酸酶对母猪和仔猪钙磷代谢的影响[D].硕士学位论文.泰安:山东农业大学, 2015. GUO W W.Effects of different dietary phosphorus levels and ratio of calcium to phosphorus with phytase addition on metabolism of sows and piglets[D].Master's Thesis.Tai'an:Shandong Agricultural University, 2015.(in Chinese)
[30] 张萌萌, 张秀珍, 邓伟民, 等.骨代谢生化指标临床应用专家共识 (2020)[J].中国骨质疏松杂志, 2020, 26(6):781-796. ZHANG M M, ZHANG X Z, DENG W M, et al.Expert consensus on clinical application of biochemical indicators of bone metabolism (2020)[J].Chinese Journal of Osteoporosis, 2020, 26(6):781-796.(in Chinese)
[31] 王德海.不同无机磷水平和植酸酶对断奶仔猪骨骼钙磷代谢及肠道相关基因表达的影响[D].硕士学位论文.泰安:山东农业大学, 2015. WANG D H.Effects of different inorganic phosphorus levels and phytase on the bone calcium and phosphorus metabolism and intestinal related gene expression of weaned piglets[D].Master's Thesis.Tai'an:Shandong Agricultural University, 2015.(in Chinese)
[32] 任跃昌.不同无机磷源及水平对产蛋后期蛋鸡生产性能、蛋品质和骨代谢的影响[D].硕士学位论文.泰安:山东农业大学, 2021. REN Y C.Effects of different inorganic phosphorus sources and levels on production performance, egg quality and bone metabolism of layers in late laying period[D].Master's Thesis.Tai'an:Shandong Agricultural University, 2021.(in Chinese)
Outlines

/