RESEARCHPAPER

Effects of Dietary Supplementation of Saccharomyces boulardii on Growth Performance, Serum Immune Indexes and Fecal Microflora of Angus Calves

  • XUE Shuaishuai ,
  • LI Yafei ,
  • LI Keyao ,
  • LIU Haoyu ,
  • HUANG Caiyun ,
  • GUO Pingting ,
  • FANG Shaoming ,
  • LIANG Xuewu ,
  • CHI Chunmei ,
  • GAN Qianfu
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  • 1. College of Animal Science (College of Bee Science), Fujian Agricultural and Forestry University, Fuzhou 350002, China;
    2. Ningde Institute of Agricultural Science, Ningde 355017, China

Received date: 2021-11-18

  Online published: 2022-06-14

Abstract

The aim of this experiment was to study the effects of dietary supplementation of Saccharomyces boulardii on growth performance, serum immune indexes and fecal microflora of Angus calves. Twenty-four Angus calves aged about (152±10) days of age with similar body weight were selected and randomly divided into 3 groups with 8 calves in each group. Calves in the control group (CG group) were fed a basal diet, and the others in experimental groups were fed the basal diet supplemented with 100 (LG group) and 200 mg/kg Saccharomyces boulardii (HG group), respectively. The pre-feeding period was 14 days and the trial period was 28 days. The results showed as follows:1) compared with the CG group, the final body weight and average daily gain of the LG and HG groups were not significantly different (P>0.05). 2) The body height of calves in the LG group was significantly higher than that in the CG group (P<0.05). 3) The serum interleukin-6 (IL-6) content of calves in LG and HG groups was extremely significant lower than that in the CG group (P<0.01), and the HG group was significantly higher than the LG group (P<0.05). The serum tumor necrosis factor-α (TNF-α) content of calves in the LG group was extremely significant lower than that in the CG group (P<0.01), and the HG group was highly significant higher than the LG group (P<0.01). 4) The Shannon index in the LG group was significantly higher than that in the CG group (P<0.05). 5) At the phylum level, the dominant phyla of three groups were Firmicutes and Bacteroidetes. At the family level, the dominant family of three groups were Ruminococcaceae, Rikenellaceae, Lachnospiraceae and Prevotellaceae etc. The relative abundance of Clostridiales_vadinBB60_group in the LG group was significantly higher than that in the CG group (P<0.05), while the relative abundance of Erysipelotrichaceae was significantly lower than that in the CG group (P<0.05). The relative abundance of Erysipelotrichaceae in the HG group was significantly higher than that in the CG group (P<0.05), while the relative abundance of Clostridiales_vadinBB60_group was significantly lower than that in the CG group (P<0.05). At the genus level, the dominant genus of three groups were Ruminococcaceae_UCG-005, Rikenellaceae_RC9_gut_group, Ruminococcaceae_UCG-010, Bacteroides and Akkermansia. The relative abundance of dgA-11_gut_group in LG and HG groups was significantly lower than that in the CG group (P<0.05). The results of LEfSe analysis showed that the relative abundance of Bacillaceae and Bacillus in LG and HG groups increased. It is concluded that dietary supplementation of Saccharomyces boulardii has no significant effect on growth performance of Angus calves, increases the body height of calves, reduces the serum proinflammatory cytokine IL-6 and TNF-α contents, and enhances the body's immunity function, raises the relative abundance of beneficial bacteria such as Bacillus, improves the fecal microflora, and promotes the health of calves. In this experiment, dietary supplementation of Saccharomyces boulardii of 100 mg/kg has the best effect.

Cite this article

XUE Shuaishuai , LI Yafei , LI Keyao , LIU Haoyu , HUANG Caiyun , GUO Pingting , FANG Shaoming , LIANG Xuewu , CHI Chunmei , GAN Qianfu . Effects of Dietary Supplementation of Saccharomyces boulardii on Growth Performance, Serum Immune Indexes and Fecal Microflora of Angus Calves[J]. Chinese Journal of Animal Nutrition, 2022 , 34(6) : 3743 -3757 . DOI: 10.3969/j.issn.1006-267x.2022.06.035

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