RESEARCHPAPER

Effects of Walnut Green Husk Extract on Ruminal Fermentation of Sheep with Subacute Ruminal Acidosis

  • CHEN Wei ,
  • CHEN Binglong ,
  • YANG Donglin ,
  • ZHANG Xiao ,
  • CHEN Yong
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  • Xinjiang Herbivore Nutrition Laboratory for Meat and Milk, College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China

Received date: 2021-11-18

  Online published: 2022-06-14

Abstract

This experiment was conducted to investigate the effects of walnut green husk extract on ruminal fermentation and lactic acid metabolism related enzyme activities of sheep under the condition of subacute ruminal acidosis (SARA) induced by high-concentrate diet. Four heathy small-tailed Han rams with average body weight of (43.3±1.5) kg and installed with permanent rumen fistula were selected in a 4×4 Latin square design. Before the feeding experiment, the SARA model was induced by the basal diet with the ratio of concentrate to roughage of 65:35. Sheep were fed the basal diet supplemented with 0 (control), 0.125%, 0.250% and 0.500% walnut green husk extract, respectively. Each phase included a 9-day preliminary trial period and an 8-day sampling period. The results showed as follows:compared with the control group, dietary supplemented with 0.125%, 0.250% or 0.500% walnut green husk extract had no significant effects on the apparent digestibility of nutrients (P>0.05), but significantly increased ruminal fluid pH (P<0.05), and significantly decreased the concentrations of total volatile fatty acids, acetate, butyrate and lactic acid and the activities of hexokinase and lactate dehydrogenase in ruminal fluid (P<0.05); dietary supplemented with 0.125% or 0.250% walnut green husk extract significantly decreased the ammonia nitrogen concentration in ruminal fluid (P<0.05); dietary supplemented with 0.250% or 0.500% walnut green husk extract significantly decreased the protozoan number and isobutyrate concentration in ruminal fluid (P<0.05); dietary supplemented with 0.125% or 0.500% walnut green husk extract significantly decreased the propionate concentration in ruminal fluid (P<0.05); dietary supplemented with 0.250% walnut green husk extract significantly decreased the propionate concentration in ruminal fluid (P<0.05); dietary supplemented with 0.125% walnut green husk extract significantly decreased the pyruvate kinase activity in ruminal fluid (P<0.05). In conclusion, walnut green husk extract can reduce the production of lactic acid, volatile fatty acid and ammonia nitrogen by reducing the activities of key enzymes in lactic acid synthesis and inhibiting the proliferation of protozoan, thus increasing the ruminal fluid pH, and improve the rumen fermentation function, alleviate the SARA in sheep induced by high-concentrate diet. The supplemental level of 0.125% walnut green husk extract is helpful to alleviate SARA in sheep.

Cite this article

CHEN Wei , CHEN Binglong , YANG Donglin , ZHANG Xiao , CHEN Yong . Effects of Walnut Green Husk Extract on Ruminal Fermentation of Sheep with Subacute Ruminal Acidosis[J]. Chinese Journal of Animal Nutrition, 2022 , 34(6) : 3824 -3834 . DOI: 10.3969/j.issn.1006-267x.2022.06.042

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