RESEARCHPAPER

Effects of Fish Meal Replacement by Enzymatic Hydrolyzed Soybean Protein Isolate on Growth Performance, Serum Biochemical Indices and Lipid Metabolism of Spotted Seabass (Lateolabrax maculatus)

  • CAO Shi ,
  • YANG Linkun ,
  • LU Kangle ,
  • ZHANG Chunxiao ,
  • WANG Ling ,
  • LI Xueshan ,
  • SONG Kai
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  • Xiamen Key Laboratory of Feed Testing and Safety Evaluation, Fisheries College, Jimei University, Xiamen 361021, China

Received date: 2021-11-27

  Online published: 2022-06-14

Abstract

The experiment was conducted to study the effects of fish meal replacement by enzymatic hydrolyzed soybean protein isolate on growth performance, serum biochemical indices and lipid metabolism of spotted seabass (Lateolabrax maculatus). A total of 225 Lateolabrax maculatus with initial body weight of (9.0±0.5) g were randomly divided into 3 groups with 3 replicates per group and 25 fish per replicate. Fish in three groups were fed with iso-nitrogenous (crude protein level was 44%) and iso-lipidic (crude lipid level was 11%) experimental diets which were prepared by using enzymatic hydrolyzed soybean protein isolate instead of fish meal of 0 (FM group), 25% (ESPI25 group) and 50% (ESPI50 group), respectively. The experimental period was 8 weeks. The results showed as follows:1) the weight gain rate (WGR), specific growth rate (SGR) and food intake (FI) of FM group were significantly higher than those of ESPI50 group (P<0.05). 2) The crude lipid apparent digestibility of FM group was significantly higher than that of ESPI50 group (P<0.05). 3) The contents of total cholesterol (TC), low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C) in serum of ESPI25 group and ESPI50 group were significantly lower than those of FM group (P<0.05). 4) The contents of triglyceride and total cholesterol in liver of ESPI50 group were significantly lower than those of FM group (P<0.05). 5) The mRNA expression levels of acetyl CoA carboxylase 1 (ACC1), fatty acid synthase (FAS), sterol regulatory element binding protein-1c (SREBP-1c), peroxisome proliferator activated receptor γ (PPARγ), 3-hydroxy-3-methylglutaryl CoA reductase (HMGCR) and carbohydrate response element binding protein (ChREBP) in liver of ESPI25 group and ESPI50 group were significantly lower than those of FM group (P<0.05). The mRNA expression levels of adenylate activated protein kinase (AMPK) and peroxisome proliferator activated receptor α (PPARα) in liver of ESPI25 group and ESPI50 group were significantly lower than those of FM group (P<0.05). In summary, the fish meal replacement by high proportion of enzymatically hydrolyzed soybean protein isolate lead to the feed intake decrease of Lateolabrax maculatus, thereby inhibiting its growth performance. However, the replacement of fish meal with enzymatically hydrolyzed soybean protein isolate in diet can inhibit the expression of fat synthesis genes by reducing contents of triglyceride and total cholesterol in serum and liver, thereby reducing the body fat deposition of Lateolabrax maculatus.

Cite this article

CAO Shi , YANG Linkun , LU Kangle , ZHANG Chunxiao , WANG Ling , LI Xueshan , SONG Kai . Effects of Fish Meal Replacement by Enzymatic Hydrolyzed Soybean Protein Isolate on Growth Performance, Serum Biochemical Indices and Lipid Metabolism of Spotted Seabass (Lateolabrax maculatus)[J]. Chinese Journal of Animal Nutrition, 2022 , 34(6) : 3884 -3895 . DOI: 10.3969/j.issn.1006-267x.2022.06.048

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