RESEARCHPAPER

Effects of “Glucose Oxidase+Catalase+Citric Acid” Combined Additives on Performance, Immune Indexes and Nutrient Apparent Digestibility of Commercial Meat Rabbits

  • ZHAN Haijie ,
  • LI Yanping ,
  • CAO Liang ,
  • DANG Wenqing ,
  • FAN Aifang ,
  • REN Keliang
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  • College of Animal Science, Shanxi Agricultural University, Taiyuan 030032, China

Received date: 2021-11-04

  Online published: 2022-06-14

Abstract

The objective of this study was to investigate the effects of different dosage of "glucose oxidase+catalase+citric acid" combined additives in the diets to replace antibiotic on the growth performance, immune organ indexes, serum immune indexes, diarrhea rate, mortality and nutrient apparent digestibility of commercial meat rabbits. Three hundred 35-day-old weaned Yila commercial meat rabbits (half male and half female) were randomly divided into 5 groups according to body weight and sex. Each group consisted of 60 replicates with 1 rabbit per replicate. The control group included a blank control group (basal diet), an antibiotic control group (basal diet+enramycin 20 g/t+aureomycin 52.5 g/t). And the experimental groups contained experimental group 1[basal diet+low dosage combined additives (glucose oxidase 200 g/t+catalase 114 g/t+citric acid 1.0%)], experimental group 2[basal diet+medium dosage combined additives (glucose oxidase 400 g/t+catalase 228 g/t+citric acid 1.0%)] and experimental group 3[basal diet+high dosage combined additives (glucose oxidase 800 g/t+ catalase 456 g/t+citric acid 1.0%)]. The experiment lasted for 35 days, of which the pre-test period was 5 days and the trial period was 30 days. The results showed as follows:1) different dosage of combined additives in the diets had no significant differences in the final body weight, average daily gain and feed/gain (P>0.05). Similarly, there was no significant difference in diarrhea rate and mortality among groups (P>0.05), but the diarrhea rate and mortality showed a downward trend with the increase of combined additives dosage. 2) The crude protein apparent digestibility of the experimental group 2 was the lowest, which was significantly different from that of antibiotic control group (P<0.05), but there was no significant difference among other groups (P>0.05). The apparent digestibility of ash, neutral detergent fiber and acid detergent fiber of the antibiotic control group were significantly (P<0.05) or extremely significantly (P<0.01) higher than those of the blank control group and three experimental groups. In addition, there was no significant difference among the blank control groups and experimental groups (P>0.05). 3) The sacculus rotundus index of experimental group 3 was the largest, which was significantly different from the antibiotic control group and experimental group 2 (P<0.01). There was significant difference in serum immunoglobulin A (IgA) content between experimental group 3 and blank control group, antibiotic control group (P<0.05). The serum secretory immunoglobulin A (sIgA) content in experimental groups 2 and 3 was extremely significantly higher than that of blank control group (P<0.01). There was significant difference in serum interleukin-10 (IL-10) content between the experimental group 3 and the blank control group (P<0.05). In conclusion, the immunity and mortality of commercial meat rabbits show an upward trend and a downward trend with the increase of "glucose oxidase+catalase+citric acid" combined additives dosage in diets, respectively. Considering the performance, nutrient apparent digestibility and immune indexes, the suitable dosage of combined additives in the diet is glucose oxidase 800 g/t+catalase 456 g/t+citric acid 1.0% under this experimental condition.

Cite this article

ZHAN Haijie , LI Yanping , CAO Liang , DANG Wenqing , FAN Aifang , REN Keliang . Effects of “Glucose Oxidase+Catalase+Citric Acid” Combined Additives on Performance, Immune Indexes and Nutrient Apparent Digestibility of Commercial Meat Rabbits[J]. Chinese Journal of Animal Nutrition, 2022 , 34(6) : 3896 -3905 . DOI: 10.3969/j.issn.1006-267x.2022.06.049

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