RESEARCHPAPER

Effects of eutR and eutB Gene Knockout on Growth and Reproduction and Biofilm Formation in Enterotoxic Escherichia coli

  • LU Xi ,
  • WU Dingyan ,
  • REN Ke ,
  • ZHU Zhenbao ,
  • QIAN Weisheng
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  • 1. School of Food and Biological Engineering, Shaanxi University of Science & Technology, Xi'an 710021, China;
    2. Tangdu Hospital, Air Force Medical University, Xi'an 710038, China

Received date: 2021-11-30

  Online published: 2022-06-14

Abstract

Ethanolamine is a potential signaling molecule, which is abundant in intestinal tract and can be utilized by intestinal microorganisms. In order to study the influence of ethanolamine on the pathogenicity of enterotoxic Escherichia coli (ETEC), firstly Red homologous recombination technology was used to knockout the eutR and eutB genes, which were key genes of the ethanolamine identify and utilization in ETEC, respectively. Subsequently, in order to investigate the effects of ETEC on growth and reproduction and biofilm formation, three ETEC strains (wild-type and eutR and eutB gene knockout strains) were treated with ethanolamine under simulated enteral hypoxia conditions. The results showed that Red technique could effectively eliminate eutR and eutB genes in ETEC. In addition, intestinal ethanolamine concentration (4 mmol/L) significantly increased the biofilms production of wild-type ETEC (P<0.05), however, the growth and propagation rate of ETEC in LB medium could not be changed. There was no such enhancement effect for eutR and eutB gene knockout strains (P>0.05). The results suggest that ETEC biofilm formation may be regulated by ethanolamine in the host intestinal ethanolamine environment, and the key genes eutR and eutB may play a key role in this process.

Cite this article

LU Xi , WU Dingyan , REN Ke , ZHU Zhenbao , QIAN Weisheng . Effects of eutR and eutB Gene Knockout on Growth and Reproduction and Biofilm Formation in Enterotoxic Escherichia coli[J]. Chinese Journal of Animal Nutrition, 2022 , 34(6) : 3930 -3939 . DOI: 10.3969/j.issn.1006-267x.2022.06.052

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