RESEARCHPAPER

Effects of Propyl-Inulin Nanoparticles as Prebiotics on Antibacterial Properties of Lactobacillus plantarum

  • LIU Xiyu ,
  • DU Lixin ,
  • WAN Ruhui ,
  • ZOU Xue ,
  • ZHANG Jianbin ,
  • PU Lei ,
  • MA Jifei ,
  • HU Debao ,
  • HONG Liang
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  • Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300392, China

Received date: 2021-11-04

  Online published: 2022-06-14

Abstract

In order to improve the antimicrobial ability of Lactobacillus plantarum, the propyl-inulin (PI) was synthesized through esterification reaction, the propyl-inulin nanoparticles (IPrN) were synthesized by self-assembly mechanism of propyl-inulin. The replacement rate of propyl in inulin was detected by 600MHz 1H-nuclear magnetic resonance (NMR) spectroscopy, and the characteristics of nanoparticles were observed by scanning electron microscopy (SEM) and dynamic light scattering (DLS). The Lactobacillus plantarum and IPrN were stained by 4',6-diamidino-2-phenylindole (DAPI) and fluorescin isothiocyanate (FITC), and the internalization result of IPrN by Lactobacillus plantarum was observed under confocal laser scanning microscope (CLSM). The antimicrobial activity of Lactobacillus plantarum internalized by IPrN against Escherichia coli K88 and Salmonella galinarum was determined by the number of viable cells of the pathogen co-culture assay and the size of inhibition zone in agar diffusion test. The growth curve and pH of Lactobacillus plantarum treated with inulin or IPrN were measured. After adding protease K into the culture medium, the agar diffusion test was conducted to test whether Lactobacillus plantarum exerted its antimicrobial ability by secreting bacteriocin. Finally, the expression level of planS of editing bacteriocin was evaluated by quantitative PCR. The results showed that the replacement rate of propyl in inulin was 76.88 mol%, and the IPrN was spherical with a diameter of 366.6 nm. The fluorescence of FITC was strongest in the center of Lactobacillus plantarum by Z-section pattern, indicating that IPrN had been internalized. Internalized stimulation of IPrN improved the antimicrobial ability of Lactobacillus plantarum. According to the growth curve and pH, the internalization of IPrN did not affect the growth and lactic acid secretion of Lactobacillus plantarum. The addition of protease K eliminated the antibacterial activity promoted by IPrN. The expression level of planS proved that the internalization of IPrN increased the expression of bacteriocin in Lactobacillus plantarum. In conclusion, propyl-inulin nanoparticles are synthesized by modifying inulin polysaccharide with propionic acid, the internalization of IPrN can improve the secretion of bacteriocin of Lactobacillus plantarum, and then improve the antibacterial ability of Lactobacillus plantarum against Escherichia coli K88 and Salmonella gallinarum.

Cite this article

LIU Xiyu , DU Lixin , WAN Ruhui , ZOU Xue , ZHANG Jianbin , PU Lei , MA Jifei , HU Debao , HONG Liang . Effects of Propyl-Inulin Nanoparticles as Prebiotics on Antibacterial Properties of Lactobacillus plantarum[J]. Chinese Journal of Animal Nutrition, 2022 , 34(6) : 3953 -3961 . DOI: 10.3969/j.issn.1006-267x.2022.06.054

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