Yeast Culture Promotes Stem Cell Proliferation and Differentiation and Improves Intestinal Epithelial Integrity of Mice

ZHANG Dexiang; ZHU Chao; ZHOU Jiayi; WANG Xiuqi

doi:10.3969/j.issn.1006-267x.2022.06.055

Chinese Journal of Animal Nutrition >

2022 , Vol. 34 >Issue 6: 3962 - 3971

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.06.055

RESEARCHPAPER

Yeast Culture Promotes Stem Cell Proliferation and Differentiation and Improves Intestinal Epithelial Integrity of Mice

ZHANG Dexiang ,
ZHU Chao ,
ZHOU Jiayi ,
WANG Xiuqi

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National Engineering Research Center for Breeding Swine Industry, Guangdong Provincial Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University, Guangzhou 510642, China

Received date: 2021-12-06

Online published: 2022-06-14

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Abstract

The aim of this study was to investigate the effects of yeast culture (YC) on crypt villus axis renewal driven by intestinal stem cells of mice. Thirty-six healthy 6-week-old male C57BL/6 mice with similar body weight[(19.85±1.45) g] were randomly divided into 3 groups with 12 replicates per group and 1 mouse per replicate. Mice in the control group were fed a basal diet, and others in 0.5% and 1.0% YC groups were fed the basal diet supplemented with 0.5% and 1.0% YC, respectively. The experiment lasted for 10 days. The results showed as follows:1) compared with the control group, the average daily feed intake (ADFI) of mice of 0.5% YC group was significantly increased (P<0.01), the average daily gain (ADG) of mice of 1.0% YC group was significantly increased (P<0.01), and the ratio of feed to gain (F/G) of mice of 1.0% YC group was significantly decreased (P<0.05). 2) Compared with the control group, the duodenum and jejunum weight per unit length of mice of 0.5% and 1.0% YC groups was significantly increased (P<0.05). 3) Compared with the control group, the activities of total superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-Px) in jejunum of mice of 0.5% and 1.0% YC groups were significantly increased (P<0.05 or P<0.01), and the jejunum malondialdehyde (MDA) content of mice of 1.0% YC group was significantly decreased (P<0.05). 4) Compared with the control group, the villus height (VH) and villus height/crypt depth (VH/CD) in jejunum of mice of 0.5% and 1.0% YC groups were significantly increased (P<0.05). 5) Compared with the control group, the fluorescence signal intensities of zonula occludens protein-1 (ZO-1) and Occludin in jejunum of mice of 0.5% and 1.0% YC groups were significantly increased (P<0.01), and the relative expression levels of ZO-1 and Occludin in jejunum of mice of 0.5% and 1.0% YC groups were significantly up-regulated (P<0.01). 6) Compared with the control group, the fluorescence signal intensity of proliferating nuclear antigen (PCNA) in jejunum of mice of 0.5% and 1.0% YC groups were significantly increased (P<0.01), while the fluorescence signal intensity of cleaved caspase-3 in jejunum of mice were significantly decreased (P<0.01). 7) Compared with the control group, the fluorescence signal intensities of keratin 20 (KRT20), Villin, chromogranin A (CGA) and positive cell number of lysozyme (LYZ) in jejunum of mice of 0.5% and 1.0% YC groups were significantly increased (P<0.05 or P<0.01). In conclusion, dietary YC can increase the proliferation and differentiation of intestinal stem cells, inhibite the cell apoptosis, improve the intestinal structure and barrier function, enhance the intestinal antioxidant capacity, and improve the growth performance of mice.

Key words： yeast culture; intestinal stem cells; intestinal epithelium; proliferation and differentiation

Cite this article

ZHANG Dexiang , ZHU Chao , ZHOU Jiayi , WANG Xiuqi . Yeast Culture Promotes Stem Cell Proliferation and Differentiation and Improves Intestinal Epithelial Integrity of Mice[J]. Chinese Journal of Animal Nutrition, 2022 , 34(6) : 3962 -3971 . DOI: 10.3969/j.issn.1006-267x.2022.06.055

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