RESEARCHPAPER

Mechanism of Quercetin on Mice Mastitis Model Based on Tumor Necrosis Factor Signaling Pathway

  • WANG Tao ,
  • CAO Lu ,
  • MI Xiaoyu ,
  • LYU Chen ,
  • ZHAO Xingxu ,
  • ZHANG Yong
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  • 1. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China;
    2. Gansu Provincial Key Laboratory of Animal Reproductive Physiology and Reproductive Regulation, Lanzhou 730070, China

Received date: 2021-11-07

  Online published: 2022-06-14

Abstract

The aim of this study was to investigate the mechanism of tumor necrosis factor (TNF) signaling pathway in mice mastitis induced by Staphylococcus aureus. Ninety female mice were dissected on the 3rd, 5th and 7th day and divided into 3 groups with 30 female mice in each group. Then, female mice in each group were divided into 5 groups:blank control group (injected with normal saline), model group (injected with bacterial solution) and quercetin high (200 mg/kg), medium (100 mg/kg) and low (50 mg/kg) dose groups. Blood samples of 6 female mice in each group were collected on days 3, 5 and 7 of treatment, respectively, to detect blood physiological indicators. Then cervical vertebra was dislocated and sacrificed. The mammary tissues were taken and pathological changes were observed by hematoxylin-eosin (HE) staining. The mRNA relative expression levels of nuclear factor inhibitory protein α (IKBα), IκB kinase β (IKKβ) and nuclear transcription factor-κB (NF-κB) were detected by real-time fluorescence quantitative PCR (qRT-PCR). The protein relative expression levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), IKKβ and NF-κB were detected by Western Blot. The results showed as follows:1) compared with blank control group, the number of white blood cells in model group was extremely significantly increased (P<0.01), the lesions of mammary tissue were serious, and the mRNA expression levels of IKBα, IKKβ and NF-κB in mammary tissue were significantly increased (P<0.05). 2) Compared with the blank control group, the mRNA relative expression levels of NF-κB and IKBα in the high dose group were not significantly different (P>0.05), and the mRNA relative expression level of IKKβ in the high dose group was not significantly different on days 3 and 7 of treatment (P>0.05). Western Blot results showed that there was no significant difference in the protein relative expression level of TNF-α between the blank control group and the high-dose group (P>0.05). The protein relative expression levels of IL-6, IKKβ and NF-κB were not significantly different on day 3 of treatment (P>0.05), but significantly different on days 5 and 7 of treatment (P<0.05). In conclusion, quercetin can inhibit the mRNA relative expression levels of IKBα, IKKβ, NF-κB and the protein relative expression levels of TNF-α, IL-6, IKKβ and NF-κB in TNF signaling pathway, and the therapeutic effect becomes better with the increase of quercetin dosage, presenting a significant dose-dependent effect. In the whole process of the occurrence and development of the disease, it conforms to the law of development of the disease. On day 7 of treatment, the period of conversion basically enter, and all indicators basically return to normal.

Cite this article

WANG Tao , CAO Lu , MI Xiaoyu , LYU Chen , ZHAO Xingxu , ZHANG Yong . Mechanism of Quercetin on Mice Mastitis Model Based on Tumor Necrosis Factor Signaling Pathway[J]. Chinese Journal of Animal Nutrition, 2022 , 34(6) : 3972 -3982 . DOI: 10.3969/j.issn.1006-267x.2022.06.056

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