RESEARCHPAPER

Alleviation Effects of Triglyceride on Intestinal Injury of Mice Induced by Enterotoxigenic Escherichia coli K88

  • PANG Lili ,
  • ZHU Rongsheng ,
  • WANG Huaizhong ,
  • WANG Jiancai ,
  • QI Jing ,
  • HUANG Baohua ,
  • HU Hongmei
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  • 1. Institute of Animal Husbandry and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, China;
    2. National Beijing Center for Drug Safety Evaluation and Research, Beijing 100850, China

Received date: 2021-11-11

  Online published: 2022-06-14

Abstract

This experiment was conducted to study the alleviation effect of triglyceride (TB) on intestinal injury of mice induced by enterotoxigenic Escherichia coli K88 (ETEC K88). Experiment 1:fifty BALB/c mice with the body weight of (20±2) g were selected and randomly divided into 5 groups with 10 mice per group. All mice fed the same basal diet, and the mice in control group were intraperitoneal injection of 0.5 mL aseptic phosphate buffer solution (PBS), while those in experimental groups were intraperitoneal injection of 0.5 mL ETEC K88 suspension with the concentrations of 5.5×108, 4.9×107, 4.0×106 and 6.5×105 CFU/mL, respectively. The clinical symptoms were observed and recorded within three days, calculated the diarrhea rate and mortality. Then, necropsied the mice to observe their intestinal pathological changes to determine the best dose of ETEC K88 for modeling. Experiment 2:one hundred and five mice with the body weight of (20±2) g were selected and randomly divided into 7 groups with 3 replicates per group and 5 mice per replicate. The negative control group intraperitoneal injection of the ETEC K88 suspension (best dose determined in experiment 1, the same as below) after fed the basal diet for 14 days, the blank control group intraperitoneal injection of aseptic PBS after fed the basal diet for 14 days, the positive control group intraperitoneal injection of ETEC K88 suspension after fed the basal diet added 0.16% chlortetracycline for 14 days, and the four experimental groups intraperitoneal injection of ETEC K88 suspension after fed the basal diet added 0.05%, 0.10%, 0.20% and 0.30% TB for 14 days. After observation for 3 days, collected blood and necropsied the mice, then detected the changes of diamine oxidase (DAO) activity, endotoxin (ET), C-reactive protein (CRP), and secretory immunoglobulin A (SIgA) contents in serum and made intestinal pathology sections to observe the intestinal histological changes. The results showed that, after intraperitoneal injection of different concentrations of ETEC K88 suspension, the mice developed diarrhea and death of varying degrees. The best dose of ETEC K88 to build mice diarrhea model was determined according to the diarrhea rate and mortality, it was 4.9×107 CFU/mL. The average daily gain of positive control group and experimental groups 1 and 2 had no significant difference compared with the blank control group (P<0.05), but significantly higher than that of negative control group (P>0.05) The diarrhea rate of mice was negative control group (53.33%)>experimental group 1 (33.33%)>positive control group (26.67%)=experimental group 2 (26.67%)>experimental group 3(20.00%)=experimental group 4(20.00%)>blank control group (no diarrhea). Degree of intestinal injury manifested as:negative control group>experimental group 1>positive control group>experimental group 2>blank control group>experimental group 4>experimental group 3. Compared with the negative control group, the serum CRP and ET content and DAO activity of experimental groups 3, 4 and positive control group were reduced in different degrees, and experimental group 3 had the biggest reduction, followed by experimental group 4. the serum CRP and ET content and DAO activity of experimental groups 3, 4 were reduced in different degrees compared with the positive control group, but there were no significant differences (P>0.05). Serum SIgA content of experimental groups 3, 4 were increased by 169.00% (P<0.05) and 114.33% (P<0.05) compared with the negative control group, respectively, and they were increased by 72.81% (P<0.05) and 37.69% (P<0.05) compared with the positive control group, respectively. In conclusion, adding 0.20% or 0.30% TB to the basal diet of mice can combat Escherichia coli infection, alleviate intestinal injury induced by ETEC K88, and the effect can be comparable to 0.16% chlortetracycline.

Cite this article

PANG Lili , ZHU Rongsheng , WANG Huaizhong , WANG Jiancai , QI Jing , HUANG Baohua , HU Hongmei . Alleviation Effects of Triglyceride on Intestinal Injury of Mice Induced by Enterotoxigenic Escherichia coli K88[J]. Chinese Journal of Animal Nutrition, 2022 , 34(6) : 4003 -4012 . DOI: 10.3969/j.issn.1006-267x.2022.06.059

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