Effects of Chronic Cold Exposure on Thermogenesis, Liver Structure and Lipid Metabolism Related Protein Pression in Mice

ZHOU Wanhui; HU Yajie; ZHANG Meng; LI Xiaoshuang; XU Bin; YANG Yuying; LI Peng; LI Shize

doi:10.3969/j.issn.1006-267x.2022.06.060

2022 , Vol. 34 >Issue 6: 4013 - 4019

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.06.060

RESEARCHPAPER

Effects of Chronic Cold Exposure on Thermogenesis, Liver Structure and Lipid Metabolism Related Protein Pression in Mice

ZHOU Wanhui ,
HU Yajie ,
ZHANG Meng ,
LI Xiaoshuang ,
XU Bin ,
YANG Yuying ,
LI Peng ,
LI Shize

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Heilong Jiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, National Experimental Teaching Demonstration Center of Veterinary Medcine, College of Animal Science Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China

Received date: 2021-11-10

Online published: 2022-06-14

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Abstract

In order to explore the effects of chronic cold exposure on thermogenesis, liver structure and lipid metabolism related protein expression in mice, thirty-six 8-week-old C57BL/6 mice with similar body weight were randomly divided into normal temperature group and chronic cold exposure group. The rats in the chronic cold exposure group were subjected to cold stimulation at 4℃ every day for 3 h at random for 21 days. After cold exposure, the contents of glucose and triglyceride in serum were detected by automatic biochemical analyzer. The morphology of liver was detected by HE staining. The content of liver glycogen was detected by PAS staining. The expression levels of lipid synthesis and decomposition related proteins in liver tissue were detected by Western blot. The results showed that compared with the normal temperature group, the serum glucose and triglyceride contents in the chronic cold exposure group decreased significantly (P<0.05), the liver vacuolar degeneration and the liver glycogen content decreased significantly (P<0.05)。The expression levels of lipid synthesis proteins sterol regulatory element binding protein 1 (SREBP1) and fatty acid synthase (FASN) in the liver tissue significantly increased (P<0.05). The expression levels of lipid decomposing protein carnitine palmitoyl transferase 1 (CPT1) and peroxisome proliferator activated receptor alpha (PPARα) increased significantly(P<0.05), and that of carnitine palmitoyl transferase 2 (CPT2) increased significantly (P<0.01). The results show that under chronic cold exposure, the consumption of serum glucose, triglyceride and liver glycogen increase and the heat production accelerate; the liver tissue is injured under stable state stress load and the expression of lipid synthesis and decomposition-related proteins in liver increase.

Key words： chronic cold exposure; liver; lipid metabolism; FASN; CPT1

Cite this article

ZHOU Wanhui , HU Yajie , ZHANG Meng , LI Xiaoshuang , XU Bin , YANG Yuying , LI Peng , LI Shize . Effects of Chronic Cold Exposure on Thermogenesis, Liver Structure and Lipid Metabolism Related Protein Pression in Mice[J]. Chinese Journal of Animal Nutrition, 2022 , 34(6) : 4013 -4019 . DOI: 10.3969/j.issn.1006-267x.2022.06.060

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