RESEARCHPAPER

Effects and Mechanism of Duck Oil Diglyceride on Weight Loss and Lipid Reduction of Obese Rats

  • SUN Yu ,
  • WANG Baowei ,
  • ZHANG Ming'ai ,
  • YANG Xun ,
  • YIN Peipei ,
  • FAN Wenlei ,
  • KONG Min
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  • 1. College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China;
    2. Nutrition and Feed Function Laboratory of National Waterfowl Industrial Technical System, Qingdao 266109, China;
    3. Shandong Food Quality and Safety Control Engineering Technology Research Center, Qingdao 266109, China;
    4. Qingdao Food Quality Safety Risk Assessment Engineering Research Center, Qingdao 266109, China

Received date: 2021-11-12

  Online published: 2022-06-14

Abstract

This experiment was conducted to investigate the effects of duck oil and duck oil diglyceride on body weight, organ indexes and serum biochemical indexes of obese rats, and the potential mechanism of anti-obesity and cholesterol-lowering effects of duck oil diglyceride. The experiment was divided into two stages. Modeling stage:eighty rats were randomly divided into 2 groups, there were 4 replicates per group and 5 rats per replicate in the control group, and there were 12 replicates in each group and 5 replicates in each replicate in the modeling group. Rats in the control group were freely fed a basal diet, and rats in the modeling group were freely fed a high fat diet containing 60% fat, continuous feeding for 28 days. Repair intervention stage:the obese rats in the modeling group were randomly divided into model control group, duck oil group and duck oil diglyceride group, with 4 replicates in each group and 5 rats in each replicate. Rats in the model control group were gavaged with distilled water and fed a high fat diet for 30 days, and rats in other groups were gavaged with duck oil and duck oil diglyceride, respectively, and fed basal diets for 30 days. The results showed as follows:1) compared with the control group, the body weight of the modeling group was significantly increased (P<0.05), indicating that the modeling was successful. 2) Compared with the model control group, the final body weight, Lee's index, liver body ratio and contents of triglyceride, total cholesterol and low density lipoprotein cholesterol in serum of duck oil diglyceride group were significantly decreased (P<0.05); the serum high density lipoprotein cholesterol was increased, but no significant difference (P>0.05); the heart body ratio, spleen body ratio, kidney body ratio and testis body ratio were no significant difference (P>0.05). 3) Compared with the model control group, the contents of triglyceride, phosphatidylcholine (PC), cholesterol ester (CE), diglyceride, acylcarnitine (CAR), lysophosphatidylcholine (LPC) and free fatty acid (FFA) in liver of duck oil diglyceride group were significantly decreased (P<0.05). The duck oil diglyceride could affect the adipocyte metabolism, thermogenesis, adipocalysis regulation, phospholipase D signaling pathway, cholesterol metabolism and other related metabolic pathways. In conclusion, duck oil diglyceride has the effect of weight loss and lipid reduction, and regulating lipid homeostasis may be the key mechanism of weight loss and lipid reduction.

Cite this article

SUN Yu , WANG Baowei , ZHANG Ming'ai , YANG Xun , YIN Peipei , FAN Wenlei , KONG Min . Effects and Mechanism of Duck Oil Diglyceride on Weight Loss and Lipid Reduction of Obese Rats[J]. Chinese Journal of Animal Nutrition, 2022 , 34(6) : 4020 -4031 . DOI: 10.3969/j.issn.1006-267x.2022.06.061

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