RESEARCHPAPER

Isolation, Identification of a Strain of Lactic Acid Bacteria with Gossypol Removing Effect and Its Fermentation and Detoxification of Cottonseed Meal

  • ZHANG Chen ,
  • MA Jiwen ,
  • WANG Yili ,
  • LI Xi ,
  • ZHU Xin ,
  • CHENG Maoji ,
  • CHEN Lijuan
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  • 1. College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China;
    2. Yili Suzhou Dairy Co., Ltd., Suzhou 215028, China;
    3. Fisugar Peptide Biology Engineering Co., Ltd., Liuan 237000, China

Received date: 2021-11-10

  Online published: 2022-06-14

Abstract

Using humus soil in cotton field as material and acetate gossypol as screening criteria, a strain with tolerance to gossypol acetate was obtained. It was identified as Pediococcus pentosaceus by physiological and biochemical and 16S rDNA gene sequence analysis. The growth curve, fermentation pH, heat resistant, antibacterial activity and effects on cottonseed meal fermentation were determined. It was found that this strain could reduce the pH of the fermentation system to about 4 within 6 hours, maintained the final pH at 3.8, and the survival rate of the bacteria was greater than 70% in the range of 37 to 45℃, and it had inhibitory effect on Escherichia coli and Staphylococcus aureus, and could remove 87.15% free gossypol and 22.83% bound gossypol from fermented cottonseed meal. Compared with the standard strain Pediococcus pentosus ATCC 33316, there was no significant difference between them on the fermentation quality in fermented cottonseed meal (P>0.05). The results show that the screened strain has high application value in the biological fermentation and detoxification of cottonseed meal, meanwhile, this study provides excellent strains and scientific basis for the development of gossypol detoxification biological preparation.

Cite this article

ZHANG Chen , MA Jiwen , WANG Yili , LI Xi , ZHU Xin , CHENG Maoji , CHEN Lijuan . Isolation, Identification of a Strain of Lactic Acid Bacteria with Gossypol Removing Effect and Its Fermentation and Detoxification of Cottonseed Meal[J]. Chinese Journal of Animal Nutrition, 2022 , 34(6) : 4050 -4059 . DOI: 10.3969/j.issn.1006-267x.2022.06.064

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