RESEARCHPAPER

Effects of Mixed Silage of Pennisetum sinese Roxb and Moutai Distiller's Grains on Its Nutrient Contents, Fermentation Quality and Microbial Diversity

  • LI Maoya ,
  • CHENG Qiming ,
  • LI Ping ,
  • FAN Xueying ,
  • CHEN Yulian ,
  • LONG Jianhua ,
  • CHEN Chao
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  • College of Animal Science, Guizhou University, Guiyang 550025, China

Received date: 2021-12-04

  Online published: 2022-06-14

Abstract

This experiment was conducted to investigate the effects of different mixing ratios of Pennisetum sinese Roxb and Moutai distiller's grains on nutrient contents, fermentation quality and microbial diversity of their silage. Four mixing ratios were set up based on fresh weight, which were 90% Pennisetum sinese Roxb+10% Moutai distiller's grains (H9J1), 80% Pennisetum sinese Roxb +20% Moutai distiller's grains (H8J2), 70% Pennisetum sinese Roxb+30% Moutai distiller's grains (H7J3) and 60% Pennisetum sinese Roxb+40% Moutai distiller's grains (H6J4), respectively. After ensiling for 45 days, the nutrient contents, fermentation quality and microorganism of the raw materials and different mixing ratio of Pennisetum sinese Roxb and Moutai distiller's grains treatments were determined. The results showed as follows:1) with the increase of the mixing ratio of Moutai distiller's grains, the contents of crude protein, lactic acid and water-soluble carbohydrate in silage were increased gradually (P<0.05), while the pH and contents of ammonia nitrogen, neutral detergent fiber, acid detergent fiber and acetic acid in silage were decreased gradually (P<0.05). Butyric acid was not detected in silage of all treatments. 2) At the phylum level, Firmicutes were the dominant phyla in all raw materials and treatments, followed by Proteobacteria; at the genus level, unclassified and other bacteria were the predominant bacteria in fresh Pennisetum sinese Roxb and Moutai distiller's grains, while Lactobacillus was the predominant bacteria in mixed silage of Pennisetum sinese Roxb and Moutai distiller's grains. With the increase of the mixing ratio of Moutai distillers' grains, the relative abundance of Lactobacillus in silage gradually decreased, and when the mixing ratio of Moutai distiller's grains was 10% to 30%, the relative abundance of Lactobacillus was more than 72.00%. In conclusion, combined with the comprehensive analysis of nutrient contents, fermentation quality and microbial diversity, the mixed silage of Pennisetum sinese Roxb and Moutai distiller's grains can improve the quality of silage. When the mixing ratio is 7:3, the quality of silage is better.

Cite this article

LI Maoya , CHENG Qiming , LI Ping , FAN Xueying , CHEN Yulian , LONG Jianhua , CHEN Chao . Effects of Mixed Silage of Pennisetum sinese Roxb and Moutai Distiller's Grains on Its Nutrient Contents, Fermentation Quality and Microbial Diversity[J]. Chinese Journal of Animal Nutrition, 2022 , 34(6) : 4071 -4080 . DOI: 10.3969/j.issn.1006-267x.2022.06.066

References

[1] 陈玲玲, 玉柱, 毛培胜, 等.中国饲草产业发展概况及饲草料质量安全现状[J].饲料工业, 2015, 36(5):56-60. CHEN L L, YU Z, MAO P S, et al.Development situation of forage grass industry and current status of forage quality safety in China[J].Feed Industry, 2015, 36(5):56-60.(in Chinese)
[2] 翟桂玉.加入WTO后我国牧草种植与草业发展的趋势[J].养殖技术顾问, 2002(6):45-46. ZHAI G Y.After joining WTO, the trend of grass cultivation and grass industry development in China[J].Technical Advisor for Animal Husbandry, 2002(6):45-46.(in Chinese)
[3] 李玉鹏, 李海花, 王倩, 等.非常规饲料资源在养羊业中的开发利用[J].饲料研究, 2019, 42(8):106-109. LI Y P, LI H H, WANG Q, et al.Development and utilization of unconventional feed resources in sheep industry[J].Feed Research, 2019, 42(8):106-109.(in Chinese)
[4] 王松, 翁吉梅, 张双翔, 等.贵州省皇竹草发展现状及建议[J].贵州畜牧兽医, 2020, 44(5):23-26. WANG S, WENG J M, ZHANG S X, et al.Development status and suggestions of emperor bamboo grass development in Guizhou province[J].Guizhou Journal of Animal Husbandry & Veterinary Medicine, 2020, 44(5):23-26.(in Chinese)
[5] 赵晓登, 陈腾达, 李玉帅, 等.皇竹草在畜牧业的应用研究进展[J].饲料研究, 2020, 43(9):157-159. ZHAO X D, CHEN T D, LI Y S, et al.Advances in applied research of Pennisetum sinese Roxb in animal husbandry[J].Feed Research, 2020, 43(9):157-159.(in Chinese)
[6] 李玉萍, 田晶晶, 张凯, 等.皇竹草粉对草鱼幼鱼生长、抗氧化反应和肠道健康的影响[J/OL].渔业科学进展:1-12.(2021-08-17)[2021-10-21].https://kns.cnki.net/kcms/detail/detail.aspx?FileName=HYSC20210811000&DbName=CAPJ2021.DOI:10.19663/j.issn2095-9869.20210326001. LI Y P, TIAN J J, ZHANG K, et al.Effects of Pennisetum sinese Roxb meal on growth, antioxidant response, and intestinal health of juvenile grass carp (Ctenopharyngodon idella)[J/OL].Progress in Fishery Sciences:1-12.(2021-08-17)[2021-10-21].https://kns.cnki.net/kcms/detail/detail.aspx?FileName=HYSC20210811000&DbName=CAPJ2021.DOI:10.19663/j.issn2095-9869.20210326001.(in Chinese)
[7] 丁飞, 王升平.日粮中添加皇竹草对母猪繁殖性能的影响[J].中国猪业, 2019, 14(4):31-35. DING F, WANG S P.The effect of the addition of Pennisetum sinese Roxb on the reproductive performance of sows in the Japanese grain[J].China Swine Industry, 2019, 14(4):31-35.(in Chinese)
[8] 梁宙宁.日粮中添加皇竹草对母猪繁殖性能的影响[D].硕士学位论文.湛江:广东海洋大学, 2016:33. LIANG Z N.The effect of the diet added in Pennisetum sinese Roxb supplementation on reproductive performance of sows[D].Master's Thesis.Zhenjiang:Guangdong Ocean University, 2016:33.(in Chinese)
[9] 吴征敏, 凡超杰, 李龙文, 等.象草与皇竹草不同比例组合对山羊瘤胃体外发酵特性的影响[J].草业科学, 2019, 36(7):1879-1889. WU Z M, FAN C J, LI W L, et al.Effects of Pennisetum purpureum and Pennisetum sinese proportions on in vitro rumen fermentation characteristics of goats[J].Pratacultural Science, 2019, 36(7):1879-1889.(in Chinese)
[10] 陈作栋, 赵二龙, 梁欢, 等.花生秧和皇竹草不同比例混合对青贮品质的影响[J].江西饲料, 2017(4):1-3. CHEN Z D, ZHAO E L, LIANG H, et al.Effect of different proportions of peanuts and Pennisetum sinese Roxb mixing on silage quality[J].Jiangxi Feed, 2017(4):1-3.(in Chinese)
[11] 王福春, 瞿明仁, 欧阳克蕙, 等.油菜秸秆与皇竹草混合微贮料对锦江黄牛体内营养物质消化率的研究[J].饲料研究, 2015(13):45-47. WANG F C, QU M R, OUYANG K H, et al.Study on the digestion rate of nutrients in Jinjiang yellow cattle by mixing rape straw with Pennisetum sinese Roxb[J].Feed Research, 2015(13):45-47.(in Chinese)
[12] 贾春旺, 原现军, 李君风, 等.青稞酒糟对紫花苜蓿和多年生黑麦草混合青贮发酵品质的影响[J].南京农业大学学报, 2016, 39(2):275-280. JIA C W, YUAN X J, LI J F, et al.Effect of adding wet hulless barley distillers' grains on fermentation quality of mixed ensilage of alfalfa and perennial ryegrass[J].Journal of Nanjing Agricultural University, 2016, 39(2):275-280.(in Chinese)
[13] 康坤.不同混合比例和贮藏延迟时间对甘薯蔓、酒糟及稻草混合青贮品质的影响[D].硕士学位论文.雅安:四川农业大学, 2013. KANG K.Effect of different mixed ratio and delayed ensiling on quality of mixed silage of sweet potato vines, distilled grains and rice straw[D].Master's Thesis.Ya'an:Sichuan Agricultural University, 2013.(in Chinese)
[14] 李芳香, 张稳, 郁建平, 等.茅台酱香型酒糟基本成分的测定与分析[J].贵州农业科学, 2016, 44(9):114-116. LI F X, ZHANG W, YU J P, et al.Determination and analysis of basic components of vinasse with Moutai-flavor type[J].Guizhou Agricultural Sciences, 2016, 44(9):114-116.(in Chinese)
[15] 内蒙古自治区质量技术监督局.青贮饲料pH值、有机酸、氨态氮测定方法:DB15/T 1458-2018[S].呼和浩特:内蒙古自治区畜牧业标准化技术委员会, 2018. Bureau of Quality and Technical Supervision of Inner Mongolia Autonomous Region.Determination of pH value, organic acid and ammonium nitrogen in silage:DB15/T 1458-2018[S].Hohhot:Animal husbandry Standardization Technical Committee of Inner Mongolia Autonomous Region, 2018.(in Chinese)
[16] 张丽英.饲料分析及饲料质量检测技术[M].北京:中国农业大学出版社, 2003:46-75. ZHANG L Y.Feed analysis and feed quality detection technology[M].Beijing:China Agricultural Science and Technology Press, 2003:46-75.(in Chinese)
[17] 韩雅珊.食品化学实验指导[M].北京:北京农业大学出版社, 1992:19-22. HAN Y S.Food chemistry experiment guide[M].Beijing:Beijing Agricultural University Press, 1992:19-22.(in Chinese)
[18] PLAYNE M J, MCDONALD P.The buffering constituents of herbage and of silage[J].Journal of the Science of Food and Agriculture, 1966, 17(6):264-268.  
[19] 傅彤.微生物接种剂对玉米青贮饲料发酵进程及其品质的影响[D].硕士学位论文.北京:中国农业科学院, 2005. FU T.The effects of microbial inoculants on the fermentation process and quality of corn silage[D].Master's Thesis.Beijing:Chinese Academy of Agricultural Sciences, 2005.(in Chinese)
[20] BLAXTER M, MANN J, CHAPMAN T, et al.Defining operational taxonomic units using DNA barcode data[J].Philosophical Transactions of the Royal Society B:Biological Sciences, 2005, 360(1462):1935-1943.  
[21] 刘志云, 钟晓霞, 姚焰础, 等.白酒糟生物饲料及其在猪生产上的应用现状[J].动物营养学报, 2020, 32(1):15-20. LIU Z Y, ZHONG X X, YAO Y C, et al.Biological feed of white distiller's grains and its application status in swine production[J].Chinese Journal of Animal Nutrition, 2020, 32(1):15-20.(in Chinese)
[22] 高晓梅, 杨会宁, 林波, 等.不同类型饲料原料混合青贮对象草青贮品质的影响[J].中国饲料, 2021(15):90-93. GAO X M, YANG H N, LIN B, et al.Effect of different types of feed materials mixed silage on silage quality of grass[J].China Feed, 2021(15):90-93.(in Chinese)
[23] 陈冬梅, 韦毅, 陈耀, 等.巨菌草与无糠壳高粱白酒糟混合青贮品质及营养成分变化[J].饲料研究, 2021, 44(2):85-88. CHEN D M, WEI Y, CHEN Y, et al.Quality and nutriention composition change of mixed Pennisetum giganteum and sorhum distiller's grain without chaff silage[J].Feed Research, 2021, 44(2):85-88.(in Chinese)
[24] CHIOU P W S, CHANG S H, YU B.The effects of wet sorghum distiller's grains inclusion on napiergrass sillage quality[J].Journal of the Science of Food and Agriculture, 2000, 80(8):1199-1205.  
[25] 任海伟, 王莉, 朱朝华, 等.白酒糟与菊芋渣混合青贮发酵品质及微生物菌群多样性[J].农业工程学报, 2020, 36(15):235-244. REN H W, WANG L, ZHU C H, et al.Ensiling co-fermentation quality and microbial community diversity of grain stiller and inulin processing residue from Helianthus tuberosus[J].Transactions of the Chinese Society of Agricultural Engineering, 2020, 36(15):235-244.(in Chinese)
[26] 阴法庭, 张凤华.饲料油菜与玉米秸秆混合青贮营养品质[J].草业科学, 2018, 35(7):1790-1796. YIN F T, ZHANG F H.Nutritional quality of silage made with different ratios of forage rape and corn stalks[J].Pratacultural Science, 2018, 35(7):1790-1796.(in Chinese)
[27] 张永辉, 张万祥, 曹蕾, 等.两种微生物添加剂对玉米青贮发酵品质及微生物数量的影响[J].甘肃畜牧兽医, 2019, 49(1):55-58, 60. ZHANG Y H, ZHANG W X, CAO L, et al.Effects of two microbial additives on the fermentation quality and number of microorganisms in corn silage[J].Gansu Animal and Veterinary Sciences, 2019, 49(1):55-58, 60.(in Chinese)
[28] 刘秦华, 张建国, 卢小良.乳酸菌添加剂对王草青贮发酵品质及有氧稳定性的影响[J].草业学报, 2009, 18(4):131-137. LIU Q H, ZHANG J G, LU X L.The effects of lactic acid bacteria inoculation on the fermentation quality and aerobic stability of king grass silage[J].Acta Prataculturae Sinica, 2009, 18(4):131-137.(in Chinese)
[29] 白元生.饲料原料学[M].北京:中国农业出版社, 1999:25-32. BAI Y S.Feed raw materials science[M].Beijing:China Agriculture Press, 1999:25-32.(in Chinese)
[30] 孙安琪.白酒糟与菊芋渣混合青贮发酵品质及微生物多样性研究[D].硕士学位论文.兰州:兰州理工大学, 2019. SUN A Q.Study on fermentation quality and microbial diversity of Jerusalem artichoke residue and grain stillage mixed silage[D].Master's Thesis.Lanzhou:Lanzhou University of Technology, 2019.(in Chinese)
[31] 孙肖慧, 原现军, 郭刚, 等.添加乙醇和糖蜜对西藏地区燕麦和紫花苜蓿混合青贮发酵品质的影响[J].畜牧兽医学报, 2014, 45(3):417-425. SUN X H, YUAN X J, GUO G, et al.Effect of adding ethanol and molasses on fermentation quality of mixed silage of oat and alfalfa in Tibet[J].Acta Veterinaria Et Zootechnica Sinica, 2014, 45(3):417-425.(in Chinese)
[32] SHAO T, OBA N, SHIMOJO M, et al.Changes in mono-and disaccharides compositions of guineagrass (Panicum maximum Jacq.) silage during early stages of ensiling[J].Journal of the Faculty of Agriculture, Kyushu University, 2003, 47(2):333-339.  
[33] 覃方锉, 赵桂琴, 焦婷, 等.不同添加剂对青贮燕麦品质的影响[J].草原与草坪, 2014, 34(1):38-43. QIN F C, ZHAO G Q, JIAO T, et al.Study on impact of different silage additives on oat silage[J].Grassland and Turf, 2014, 34(1):38-43.(in Chinese)
[34] 原现军, 余成群, 夏坤, 等.添加青稞酒糟对西藏箭筈豌豆与苇状羊茅混合青贮发酵品质的影响[J].畜牧兽医学报, 2012, 43(9):1408-1414. YUAN X J, YU C Q, XIA K, et al.Effect of adding wet hullessbarley distillers' grains on fermentation quality of mixed silage of common vetch (Vicia sativa) and tall fescue (Festuca arundinacea) in Tibet[J].Acta Veterinaria Et Zootechnica Sinica, 2012, 43(9):1408-1414.(in Chinese)
[35] 张娟, 史文娇, 韩雪林, 等.苹果酸和嗜酸乳杆菌对圆叶决明青贮品质及微生物多样性的影响[J].动物营养学报, 2021, 33(12):6941-6952. ZHANG J, SHI W J, HAN X L, et al.Effects of malic acid and Lactobacillus acidophilus on quality and microbial diversity of Chamaecrista rotundifolia silage[J].Chinese Journal of Animal Nutrition, 2021, 33(12):6941-6952.(in Chinese)
[36] 陶莲, 刁其玉.青贮发酵对玉米秸秆品质及菌群构成的影响[J].动物营养学报, 2016, 28(1):198-207. TAO L, DIAO Q Y.Effects of ensiling on quality and bacteria composition of corn stalk[J].Chinese Journal of Animal Nutrition, 2016, 28(1):198-207.(in Chinese)
[37] 杨文琦, 龙宣杞, 崔卫东.玉米青贮中细菌多样性分析[J].新疆农业科学, 2013, 50(8):1424-1433. YANG W Q, LONG X Q, CUI W D.Bacteria diversity analysis in corn silage[J].Xinjiang Agricultural Sciences, 2013, 50(8):1424-1433.(in Chinese)
[38] TOHNO M, KITAHARA M, IRISAWA T, et al.Lactobacillus silagei sp. nov., isolated from orchardgrass silage[J].International Journal of Systematic and Evolutionary Microbiology, 2013, 63(Pt.12):4613-4618.
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