RESEARCH PAPER

Effects of 2-Hydroxy-4-Methionine Isopropyl on Ruminal Fermentation and Microflora of Peripartum Dairy Cows

  • MA Liqin ,
  • WANG Ling ,
  • WEN Wan ,
  • TIAN Jia ,
  • ZHOU Jiamin ,
  • WANG Fenglian ,
  • MA Xulin ,
  • CHEN Hao
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  • 1. College of Agriculturel, Ningxia University, Yinchuan 750021, China;
    2. Ningxia Hui Autonomous Region Animal Husbandry Workstation, Yinchuan 750002, China

Received date: 2021-12-07

  Online published: 2022-07-14

Abstract

This experiment was conducted to investigate the effects of 2-hydroxy-4-methionine isopropyl (HMBi) on ruminal fermentation and microflora of peripartum dairy cows. Forty Holstein cows (similar in age, body condition, number of lactation, due date and milk yield of last parity) was distinguished into control group and test group, 20 head per group, and kept in the same condition. The control group fed a basal diet, and the test group fed the basal diet with 30 g/d HMBi in the pre-perinatal period. Both control group and test group were fed a common diet in post-perinatal period, and this study lasted 44 days. Six cows were selected from each group, and the rumen fluid was collected from rumen on pre-perinatal 7 d (-7 d) and post perinatal 7 d (+7 d) before morning feeding, to determine the changes of ruminal fermentation and microflora before and after parturition. The results showed as follows:1) compared with control group, adding HMBi significantly reduced the concentration of ammoniacal nitrogen (NH3-N) in the pre-perinatal period (P<0.05), and it had no significant effects on the pH, microbial protein (MCP) content, total volatile fatty acid (TVFA), acetic acid, propionic acid, butyric acid concentrations and acetic acid/propionic acid (P>0.05). 2) Compared with the control group, HMBi was significantly increased the relative abundances of Kiritimatiellaeota, Succinivibrionaceae_UCG-001 and Syntrophococcus in the pre-perinatal period (P<0.05), and significantly improved the relative abundances of Fibrobacteres, Ruminococcaceae_UCG-005, Ruminococcus_1, Anaerovorax in post-perinatal period (P<0.05). The relative abundance of Proteobacteria in rumen of dairy cows in test group was significantly decreased (P<0.01). It is concluded that adding HMBi can increase the utilization rate of NH3-N by rumen microorganisms and promote the synthesis of MCP. HMBi can improve the digestibility of dietary fiber, starch and other substances by rumen bacteria, and enhance the ability of rumen bacteria to adapt to the structure of postpartum high-starch diets, and HMBi had a positive effect on maintain the balance of the rumen flora.

Cite this article

MA Liqin , WANG Ling , WEN Wan , TIAN Jia , ZHOU Jiamin , WANG Fenglian , MA Xulin , CHEN Hao . Effects of 2-Hydroxy-4-Methionine Isopropyl on Ruminal Fermentation and Microflora of Peripartum Dairy Cows[J]. Chinese Journal of Animal Nutrition, 2022 , 34(7) : 4425 -4437 . DOI: 10.3969/j.issn.1006-267x.2022.07.033

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