Composition Characteristics Analysis of Intestinal Bacterial Flora for Healthy and Diarrhea Yak Calves under Different Rearing Methods

ZHANG Yuying; LIU Yanhong; GUO Wenjie; HAO Wenjun; LIU Shujie; FENG Yuzhe; CUI Zhanhong

doi:10.3969/j.issn.1006-267x.2022.07.041

2022 , Vol. 34 >Issue 7: 4519 - 4529

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.07.041

RESEARCH PAPER

Composition Characteristics Analysis of Intestinal Bacterial Flora for Healthy and Diarrhea Yak Calves under Different Rearing Methods

ZHANG Yuying ,
LIU Yanhong ,
GUO Wenjie ,
HAO Wenjun ,
LIU Shujie ,
FENG Yuzhe ,
CUI Zhanhong

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Qinghai Provincial Key Laboratory of Animal Nutrition and Feed Science for Plateau Grazing Livestock, Qinghai Yak Engineering Research Center, College of Animal Science and Veterinary Sciences, Qinghai University, Xining 810016, China

Received date: 2021-12-12

Online published: 2022-07-14

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Abstract

This experiment was conducted to investigate the intestinal bacterial flora composition characteristics of healthy and diarrhea yak calves under two rearing methods-grazing and stall-feeding. Twelve 3-month-old yak calves (male) were selected under grazing and sheltering conditions, respectively, including six healthy yak calves and six diarrhea calves under grazing and six healthy yak calves and six diarrhea calves under stall-feeding. Fecal samples of all the calves were collected by rectal sampling method, and the high quality of bacterial total DNA was extracted. 16S rRNA sequencing technology was used to analyze the diversity and richness of intestinal bacterial flora of healthy and diarrhea yak calves under two rearing methods. The results showed as follows:there were no significant differences in Shannon, Simpson and Chao1 indexes between healthy yak calves and diarrhea yak calves (P>0.05), except ACE index of diarrhea yak calves was significantly higher than that of healthy yak calves (P<0.05). At the same time, Shannon and Simpson indexes of grazing yak calves were significantly higher than those of stall-feeding yak calves (P<0.05). At phylum level, the relative abundance of Desulfobacterota in intestinal bacterial flora of diarrhea yak calves under grazing condition was significantly higher than that of healthy yak calves (P<0.05). At genus level, the relative abundances of Pasteurella and Mailhella in intestinal bacterial flora of diarrhea yak calves under grazing condition was significantly higher than those of healthy yak calves (P<0.05); the relative abundances of Pasteurella and Satterella in intestinal bacterial flora of diarrhea yak calves were significantly higher than those of healthy yak calves under stall-feeding condition (P<0.05). In conclusion, grazing at pasture with the nursing mother can increase the intestinal bacterial flora diversity of yak calves when compared with stall-feeding with milk replacer; the intestinal bacterial flora composition of health and diarrhea yak calves have a certain differences at genus level under the two rearing methods, and in both grazing and stall-feeding conditions, relative abundances of Pasteurella in intestinal bacterial flora of diarrhea yak calves is significantly higher than that of healthy yak calves, which shows that Pasteurella is the common characteristic of diarrhea in yak calves under the two rearing methods.

Key words： yak calves; rearing method; diarrhea; feces; intestinal bacterial flora

Cite this article

ZHANG Yuying , LIU Yanhong , GUO Wenjie , HAO Wenjun , LIU Shujie , FENG Yuzhe , CUI Zhanhong . Composition Characteristics Analysis of Intestinal Bacterial Flora for Healthy and Diarrhea Yak Calves under Different Rearing Methods[J]. Chinese Journal of Animal Nutrition, 2022 , 34(7) : 4519 -4529 . DOI: 10.3969/j.issn.1006-267x.2022.07.041

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